5-[2-[(t-butoxylcarbonyl)amino]-1-thioethyl]-pentyl 5-O-(2,3,5-tri-O-benzoyl-α-D-arabinofuranosyl)-2,3-di-O-benzoyl-α-D-arabinofuranoside | 1255529-75-9

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 英文名称: 5-[2-[(t-butoxylcarbonyl)amino]-1-thioethyl]-pentyl 5-O-(2,3,5-tri-O-benzoyl-α-D-arabinofuranosyl)-2,3-di-O-benzoyl-α-D-arabinofuranoside
• CAS: 1255529-75-9
• 化学式: C₅₇H₆₁NO₁₆S
• 分子量: 1048.17
• InChiKey: DNBIMGXEDJDSFL-FRFFDNHOSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  8.66
• 重原子数：
  75.0
• 可旋转键数：
  24.0
• 环数：
  7.0
• sp3杂化的碳原子比例：
  0.37
• 拓扑面积：
  206.75
• 氢给体数：
  1.0
• 氢受体数：
  17.0

反应信息

• 作为反应物：
  描述：

  5-[2-[(t-butoxylcarbonyl)amino]-1-thioethyl]-pentyl 5-O-(2,3,5-tri-O-benzoyl-α-D-arabinofuranosyl)-2,3-di-O-benzoyl-α-D-arabinofuranoside 在 甲醇 、 sodium methylate 作用下， 以 二氯甲烷 为溶剂， 反应 2.0h， 以81.5%的产率得到5-[2-[(t-butoxylcarbonyl)amino]-1-thioethyl]-pentyl 5-O-(α-D-arabinofuranosyl)-α-D-arabinofuranoside
  参考文献：
  名称：

  Development of a plate-based scintillation proximity assay for the mycobacterial AftB enzyme involved in cell wall arabinan biosynthesis

  摘要：

  A number of mycobacterial arabinosyltransferases, such as the Emb proteins, AftA, AftB, AftC, and AftD have been characterized and implicated to be involved in the cell wall arabinan assembly. These arabinosyltransferases are essential for the viability of the organism and are logically valid targets for developing new anti-tuberculosis agents. For instance, Ethambutol, a first line anti-tuberculosis drug, targets the Emb proteins involved in the formation of the arabinan of cell wall arabinogalactan. Among these arabinosyltransferases, the terminal beta-(1 -> 2) arabinosyltransferase activity has been associated with AftB. The predicted topology of AftB in Mycobacterium tuberculosis has 10 N terminal transmembrane domains and a C terminal hydrophilic domain similar to the Emb proteins. It has a conserved GT-C motif and is difficult to express. In a cell free assay, synthetic disaccharide, alpha-D-Araf-(1 -> 5)-alpha-D- Araf-octyl, has been used as a substrate to explore the function of AftB. In our work, the disaccharide was synthesized in its pentenylated and biotinylated form, and the enzymatic product formed was identified as the beta-(1 -> 2) arabinofuranose adduct. When synthetic tri- and tetra-saccharides were used as substrates, a mixture of products containing both beta-(1 -> 2) and alpha-(1 -> 5) linkages were formed. Therefore, the biotinylated disaccharide was selected to develop a scintillation proximity assay. (C) 2010 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.bmc.2010.07.040
• 作为产物：
  描述：

  5-[2-[(t-butoxylcarbonyl)amino]-1-thioethyl]-pentyl 2,3-di-O-benzoyl-α-D-arabinofuranoside 、 2,3,5-tri-O-benzoyl-α-D-arabinofuranosyl trichloroacetimidate 在 三氟甲磺酸三甲基硅酯 作用下， 以 二氯甲烷 为溶剂， 反应 1.0h， 以86.5%的产率得到5-[2-[(t-butoxylcarbonyl)amino]-1-thioethyl]-pentyl 5-O-(2,3,5-tri-O-benzoyl-α-D-arabinofuranosyl)-2,3-di-O-benzoyl-α-D-arabinofuranoside
  参考文献：
  名称：

  Development of a plate-based scintillation proximity assay for the mycobacterial AftB enzyme involved in cell wall arabinan biosynthesis

  摘要：

  A number of mycobacterial arabinosyltransferases, such as the Emb proteins, AftA, AftB, AftC, and AftD have been characterized and implicated to be involved in the cell wall arabinan assembly. These arabinosyltransferases are essential for the viability of the organism and are logically valid targets for developing new anti-tuberculosis agents. For instance, Ethambutol, a first line anti-tuberculosis drug, targets the Emb proteins involved in the formation of the arabinan of cell wall arabinogalactan. Among these arabinosyltransferases, the terminal beta-(1 -> 2) arabinosyltransferase activity has been associated with AftB. The predicted topology of AftB in Mycobacterium tuberculosis has 10 N terminal transmembrane domains and a C terminal hydrophilic domain similar to the Emb proteins. It has a conserved GT-C motif and is difficult to express. In a cell free assay, synthetic disaccharide, alpha-D-Araf-(1 -> 5)-alpha-D- Araf-octyl, has been used as a substrate to explore the function of AftB. In our work, the disaccharide was synthesized in its pentenylated and biotinylated form, and the enzymatic product formed was identified as the beta-(1 -> 2) arabinofuranose adduct. When synthetic tri- and tetra-saccharides were used as substrates, a mixture of products containing both beta-(1 -> 2) and alpha-(1 -> 5) linkages were formed. Therefore, the biotinylated disaccharide was selected to develop a scintillation proximity assay. (C) 2010 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.bmc.2010.07.040