leucovorin

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 英文名称: leucovorin
• 英文别名: (2S)-2-[[4-[(2-amino-5-formyl-4-oxo-3,4a,6,7-tetrahydropteridin-6-yl)methylamino]benzoyl]amino]pentanedioic acid
• 化学式: C₂₀H₂₃N₇O₇
• 分子量: 473.445
• InChiKey: AFFDJSNPCNCZFO-OWYJLGKBSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -1.7
• 重原子数：
  34
• 可旋转键数：
  9
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.35
• 拓扑面积：
  216
• 氢给体数：
  6
• 氢受体数：
  9

反应信息

• 作为产物：
  描述：

  Folic acid 在 sodium hydroxide 、 锌 作用下， 反应 9.0h， 生成 leucovorin
  参考文献：
  名称：

  Production of Dihydrofolate Reductase by ClonedEscherichia coliand Its Application to Asymmetric Synthesis ofl-Leucovorin

  摘要：

  We have investigated culture conditions for production of dihydrofolate reductase by Escherichia coli harboring a high expression plasmid, pTP64–1. Sorbitol addition and pH control were effective for the production of the enzyme in a jar fermentor. The enzyme was purified from a cell-free extract by column chromatographies on DEAE-Cellulofine and Superose Prep12 and showed a single band on SDS-polyacrylamide gel electrophoresis.The reduction of 200 mM dihydrofolate to 6(S)-tetrahydrofolate, an intermediate for l-leucovorin synthesis, was complete in 2 hr under anaerobic conditions, using 1.5 units/ml of the purified enzyme.

  DOI：

  10.1271/bbb.56.437

文献信息

• Production of Dihydrofolate Reductase by Cloned<i>Escherichia coli</i>and Its Application to Asymmetric Synthesis of<i>l</i>-Leucovorin
  作者：Takashi Ohshiro、Yukihiro Kuge、Akiko Igarashi、Kenichi Mochida、Masanori Iwakura、Takayuki Uwajima

  DOI：10.1271/bbb.56.437

  日期：1992.1

  We have investigated culture conditions for production of dihydrofolate reductase by Escherichia coli harboring a high expression plasmid, pTP64–1. Sorbitol addition and pH control were effective for the production of the enzyme in a jar fermentor. The enzyme was purified from a cell-free extract by column chromatographies on DEAE-Cellulofine and Superose Prep12 and showed a single band on SDS-polyacrylamide gel electrophoresis.The reduction of 200 mM dihydrofolate to 6(S)-tetrahydrofolate, an intermediate for l-leucovorin synthesis, was complete in 2 hr under anaerobic conditions, using 1.5 units/ml of the purified enzyme.