硫酸壮观霉素 | 23312-56-3

• 物质功能分类: 分析化学 - 标准品 - 药典标准品和杂质标准品
• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 有机硫酸及其衍生物
• 中文名称: 硫酸壮观霉素
• 英文名称: spectinomycin sulfate tetrahydrate
• 英文别名: spectinomycin；spectinomycin sulfate；sulfuric acid;(1R,3S,5R,8R,10R,11S,12S,13R,14S)-8,12,14-trihydroxy-5-methyl-11,13-bis(methylamino)-2,4,9-trioxatricyclo[8.4.0.03,8]tetradecan-7-one
• CAS: 23312-56-3
• 化学式: C₁₄H₂₄N₂O₇*H₂O₄S
• mdl: MFCD00270185
• 分子量: 430.433
• InChiKey: XGBFWQUQYQIFLB-MTTMTQIXSA-N

物化性质

• 熔点：
  ~185° (dec)
• 比旋光度：
  D25 +17.0° (c = 1 in water)
• 溶解度：
  DMSO（少许）、水（少许）

计算性质

• 辛醇/水分配系数(LogP)：
  -2.24
• 重原子数：
  28
• 可旋转键数：
  2
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.928
• 拓扑面积：
  213
• 氢给体数：
  7
• 氢受体数：
  13

制备方法与用途

用途 

壮观霉素系广谱性抗生素,对革兰氏阳性菌及阴性菌均有较强的抑制能力,对金黄色葡萄球菌、溶血性链球菌、肺炎双球菌、普通变形杆菌、肺炎杆菌等以及支原体微浆菌和某些孤菌均有效。

反应信息

• 作为反应物：
  描述：

  硫酸壮观霉素 在 盐酸 、 叔丁基过氧化氢 、 tris(dibenzylideneacetone)dipalladium (0) 、 碳酸氢钠 、 三乙胺 、 三苯基膦 、 copper dichloride 、 lithium hexamethyldisilazane 作用下， 以 二氯甲烷 、 水 、 丙酮 、 乙腈 为溶剂， 反应 11.0h， 生成 [(4aR,5S,6S,7R,8S,8aR,9aR,10aR)-5-(Benzyloxycarbonyl-methyl-amino)-2-but-3-enyl-4a,6,8-trihydroxy-4-oxo-4a,5,6,7,8,8a,9a,10a-octahydro-4H-1,9,10-trioxa-anthracen-7-yl]-methyl-carbamic acid benzyl ester
  参考文献：
  名称：

  Oxidation and alkylation of spectinomycin derivatives: synthesis of trospectomycin from spectinomycin

  摘要：

  Trospectomycin can be prepared in five steps from spectinomycin. The overall yield of the process is 13.3%. The synthesis involves a new oxidation of silyl enol ether 4 to enone 5 by use of an alkyl hydroperoxide. This reaction is mild and very selective, giving excellent yields. The synthesis also involves a gamma-alkylation of enone 5 activated by a beta-oxygen. This alkylation is unusual in that no alkylation is seen at the a (4') carbon.

  DOI：

  10.1021/jo00055a021

文献信息

• Veterinary preparation containing an antibiotic mixture of gentamicin and lincomycin as an additive to drinking water or animal feed and its use in pig breeding
  申请人：LEK, tovarna farmacevtskih in kemicnih izdelkov, d.d., Ljubljana

  公开号：EP0597167A1

  公开（公告）日：1994-05-18

  Described is new veterinary preparation, which is added to drinking water or to animal feed for pigs and contains 1 part by weight of lincomycin and 1 to 10 parts by weight of gentamicin or their pharmaceutically acceptable acid addition salts in a usual carrier for the use in the prophylaxis and therapy of dysentery and colibacilliosis and in the prophylaxis of enzootic pneumonia of pigs and hence for increasing the gain in their body weight. The new veterinary preparation for pig breeding has shown improved characteristics reflecting in a reduction of total losses and deaths with symptoms of diarrhoea and pneumonia, in a decrease of the percentage of poorly developed pigs and in the absence of their deaths, in a decrease of the conversion, and in an improvement of the average daily gain in the body weight.

  所述新兽药制剂添加到猪的饮用水或动物饲料中，含有 1 份（按重量计）林可霉素和 1 至 10 份（按重量计）庆大霉素或它们的药学上可接受的酸加成盐，以常用载体为基 础，用于预防和治疗猪的痢疾和大肠杆菌病，以及预防猪的猪肺疫，从而提高猪的增重。 这种用于养猪的新型兽药制剂在以下方面表现出了更好的特性：减少了总损失和因腹泻和肺炎症状而死亡的猪只；降低了发育不良猪只的比例；减少了死亡猪只；降低了转化率；提高了平均日增重。
• Peptide-based method for monitoring gene expression in a host cell
  申请人：Friedrich-Alexander-Universität Erlangen-Nürnberg

  公开号：EP1580273A1

  公开（公告）日：2005-09-28

  The present invention relates a method for monitoring the expression level of a gene in a host cell by modulating the activity of a regulatory biomolecule, comprising the steps of: (a) transforming a cell expressing a regulatory biomolecule with a nucleic acid molecule comprising an open reading frame encoding an interaction partner of said biomolecule in expressible form, wherein (i) said regulatory biomolecule is either a nucleic acid binding molecule that effects its regulatory activity upon binding or an allosterically controlled ribonucleic acid molecule; and (ii) the interaction partner of the biomolecule is encoded by a nucleic acid molecule comprising: (1) a nucleic acid sequence encoding a tagged (poly)peptide, or (2) a nucleic acid sequence encoding a tagged (poly)peptide or a peptide tag, a selectable marker gene and additional nucleotide sequences for site specific, in-frame integration of said nucleic acid molecule into the coding sequence of at least one host (poly)peptide of interest, wherein said tag comprises the interacting residues of the interaction partner and (b) assessing the expression level of the gene. Furthermore, the present invention relates to a method of producing and/or selecting a compound capable of modulating the activity of a nucleic acid binding protein comprising the steps of: (a) conducting a selection of compounds with the nucleic acid binding target protein under conditions allowing an interaction of the compound and the nucleic acid binding protein; (b) removing unspecifically bound compounds; (c) detecting specific binding of compounds to the nucleic acid binding target protein; (d) expressing in a cell, the nucleic acid binding protein and providing in trans the coding sequence of at least one indicator gene, wherein said coding sequence is under control of the target sequence of the nucleic acid binding protein; (e) adding a candidate compound to the cell of step (d); (f) determining the amount or activity of the indicator protein, wherein a reduced or increased amount of indicator protein is indicative of compounds, capable of modulating the activity of the nucleic acid binding protein; (g) selecting compounds capable of modulating the activity of the nucleic acid binding protein. Moreover, the present invention relates to nucleic acid molecules, polypeptides, expression vectors, host cells, ensembles of host cells and a non-human animal comprising said host cells. Finally, the present invention relates to a kit comprising the nucleic acid molecule, the (poly)peptide, the vector, the host cell or the ensemble of host cells of the present invention in one or more containers.

  本发明涉及一种通过调节调控生物分子的活性来监测宿主细胞中基因表达水平的方法，包括以下步骤：(a) 用包含开放阅读框的核酸分子转化表达调控生物大分子的细胞，该开放阅读框以可表达的形式编码所述生物大分子的相互作用伙伴，其中(i) 所述调控生物大分子是一种核酸结合分子，结合后可影响其调控活性，或者是一种异位控制的核糖核酸分子；以及(ii) 生物大分子的相互作用伙伴由核酸分子编码，该核酸分子包含：(1)编码标记(多)肽的核酸序列，或(2)编码标记(多)肽或肽标签的核酸序列、可选择标记基因和附加核苷酸序列，用于将所述核酸分子定点、框内整合到至少一种感兴趣的宿主(多)肽的编码序列中，其中所述标签包括相互作用伙伴的相互作用残基；以及(b)评估基因的表达水平。此外，本发明还涉及一种生产和/或筛选能够调节核酸结合蛋白活性的化合物的方法，包括以下步骤：(a) 在允许化合物与核酸结合蛋白相互作用的条件下，对与核酸结合靶蛋白结合的化合物进行筛选； (b) 去除非特异性结合的化合物； (c) 检测化合物与核酸结合靶蛋白的特异性结合； (d) 在细胞中表达核酸结合蛋白，并反式提供至少一个指示基因的编码序列，其中所述编码序列受核酸结合蛋白靶序列的控制；(e) 向步骤(d)的细胞中加入候选化合物； (f) 确定指示蛋白的量或活性，其中指示蛋白量的减少或增加表明化合物能够调节核酸结合蛋白的活性； (g) 选择能够调节核酸结合蛋白活性的化合物。此外，本发明还涉及核酸分子、多肽、表达载体、宿主细胞、宿主细胞组合以及包含所述宿主细胞的非人类动物。最后，本发明涉及一种试剂盒，该试剂盒包含本发明的核酸分子、（多）肽、载体、宿主细胞或宿主细胞组合，装在一个或多个容器中。
• Transponson-mediated mutagenesis in spermatogonial stem cells
  申请人：Max Delbrück Centrum für Molekulare Medizin (MDC) Berlin-Buch;

  公开号：EP2067402A1

  公开（公告）日：2009-06-10

  The present invention relates to a method for generating mutations in spermatogonial stem cells comprising introducing at least one transposon into the spermatogonial stem cells. The invention further relates to cells obtainable by the method of the invention, a method for linking physiological data with genetic information as well as a transposon. Further, the invention relates to a method of generating non-human animals comprising the mutated spermatogonial stem cells as well as to a anon-human animals comprising the mutated spermatogonial stem cell of the invention. Furthermore, the invention relates to a method of saturating a genetic locus with mutations in a spermatogonial stem cell and finally, the invention relates to a spermatogonial stem cell library comprising mutated spermatogonial stem cells of the invention.

  本发明涉及一种在精原干细胞中产生突变的方法，包括将至少一个转座子导入精原干细胞。本发明还涉及可通过本发明方法获得的细胞、将生理数据与遗传信息以及转座子联系起来的方法。此外，本发明还涉及一种产生包含突变精原干细胞的非人类动物以及包含本发明突变精原干细胞的非人类动物的方法。此外，本发明还涉及一种使精原干细胞中的基因位点饱和突变的方法，最后，本发明还涉及一种包含本发明突变精原干细胞的精原干细胞库。
• Recombinant strain producing O-aminobenzoate and fermentative production of aniline from renewable resources via 2-aminobenzoic acid
  申请人：Covestro Deutschland AG

  公开号：US10731187B2

  公开（公告）日：2020-08-04

  The invention provides a recombinant microbial host cell capable of converting a raw material comprising a fermentable carbon substrate to o-aminobenzoate biologically. The invention further provides a method for producing aniline, comprising the steps of: a) producing o-aminobenzoate by fermentation of a raw material comprising at least one fermentable carbon substrate using the recombinant microbial host cell of the capable of converting said raw material comprising at least one fermentable carbon substrate to o-aminobenzoate biologically, wherein said o-aminobenzoate comprises anthranilate anion, b) converting said o-aminobenzoate from said anthranilate anion to anthranilic acid by acid protonation, c) recovering said anthranilic acid by precipitation or by dissolving in an organic solvent, and d) converting said anthranilic acid to aniline by thermal decarboxylation in an organic solvent.

  本发明提供了一种重组微生物宿主细胞，该细胞能够将包含可发酵碳底物的原料生物转化为邻氨基苯甲酸酯。本发明进一步提供了一种生产苯胺的方法，包括以下步骤a) 利用重组微生物宿主细胞将包含至少一种可发酵碳底物的原料发酵生产邻氨基苯甲酸盐，其中所述邻氨基苯甲酸盐包含蒽酸阴离子、b) 通过酸质子化将所述邻氨基苯甲酸从所述蚁酸阴离子转化为蚁酸， c) 通过沉淀或溶解在有机溶剂中回收所述蚁酸，以及 d) 通过在有机溶剂中热脱羧将所述蚁酸转化为苯胺。
• Stable VAMP reporter assay
  申请人：THE UNIVERSITY OF SHEFFIELD

  公开号：US11345945B2

  公开（公告）日：2022-05-31

  The present invention provides a polypeptide comprising an N-terminal polypeptide domain having luciferase activity and a C-terminal polypeptide domain having VAMP1, VAMP2 or VAMP3 activity where VAMP stands for vesicle-associated membrane protein. Corresponding nucleic acid molecules, expression vectors and genetically modified cells are also provided. The invention also provides methods and uses of the same.

  本发明提供了一种多肽，它包含具有荧光素酶活性的 N 端多肽结构域和具有 VAMP1、VAMP2 或 VAMP3 活性的 C 端多肽结构域，其中 VAMP 代表囊泡相关膜蛋白。本发明还提供了相应的核酸分子、表达载体和转基因细胞。本发明还提供了相应的方法和用途。