[[5-(2,4-二氧代嘧啶-1-基)-3-羟基-四氢呋喃-2-基]甲氧基-羟基-磷酰]氧基膦酸 | 4208-67-7

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘧啶核苷酸
• 中文名称: [[5-(2,4-二氧代嘧啶-1-基)-3-羟基-四氢呋喃-2-基]甲氧基-羟基-磷酰]氧基膦酸
• 中文别名: 2-(8-十七碳烯基)-N-[2-[2-(8-十七碳烯基)-4,5-二氢-1H-咪唑-1-基]乙基]-4,5-二氢-1H-咪唑-1-乙基胺
• 英文名称: dUDP
• 英文别名: 2'-Deoxyuridine-5'-diphosphate；Deoxyuridine-5'-diphosphate；[(2R,3S,5R)-5-(2,4-dioxopyrimidin-1-yl)-3-hydroxyoxolan-2-yl]methyl phosphono hydrogen phosphate
• CAS: 4208-67-7
• 化学式: C₉H₁₄N₂O₁₁P₂
• 分子量: 388.164
• InChiKey: QHWZTVCCBMIIKE-SHYZEUOFSA-N

物化性质

• 密度：
  1.894±0.06 g/cm3(Predicted)
• 物理描述：
  Solid

计算性质

• 辛醇/水分配系数(LogP)：
  -4.4
• 重原子数：
  24
• 可旋转键数：
  6
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.56
• 拓扑面积：
  192
• 氢给体数：
  5
• 氢受体数：
  11

反应信息

• 作为产物：
  描述：

  2'-脱氧胸苷-5'-三磷酸三钠二水 在 Mycobacterium tuberculosis 、 5’-三磷酸腺苷 作用下， 生成 [[5-(2,4-二氧代嘧啶-1-基)-3-羟基-四氢呋喃-2-基]甲氧基-羟基-磷酰]氧基膦酸
  参考文献：
  名称：

  胸苷和胸苷5'-O-单磷酸酯类似物可作为结核分枝杆菌胸苷酸激酶的抑制剂。

  摘要：

  评估了一系列2'，3'-和5-修饰的胸苷类似物对结核分枝杆菌胸苷单磷酸激酶（TMPKmt）的亲和力。几种非磷酸化类似物的亲和力与其磷酸化同类物的亲和力处于相同的数量级。考虑到与磷酸化化合物有关的药物传递问题，这些“游离”核苷似乎在寻找TMPKmt抑制剂作为新型抗结核药方面更具前景。

  DOI：

  10.1016/s0960-894x(03)00643-7

文献信息

• Sheet or tubular structure consisting of elastic biocompatible material and its use
  申请人：Charité - Universitätsmedizin Berlin

  公开号：EP2067494A1

  公开（公告）日：2009-06-10

  The present invention relates to a sheet or tubular structure consisting of biocompatible material, which is elastic and comprises at least one biologically active substance in at least one region and to a sheet or tubular structure, which comprises at least one biologically active substance release-modifying agent in at least one region as well as an implant covered at least partially by the sheet or tubular structure of the invention. The present invention further provides a method and an apparatus for producing, and preferably customizing and/or optimizing, the sheet or tubular structure of the invention. The sheet or tubular structure of the invention can be customized/optimized before implantation in the operating room.

  本发明涉及一种由生物相容性材料构成的片状或管状结构，该结构具有弹性，并在至少一个区域中包含至少一种生物活性物质；本发明还涉及一种片状或管状结构，该结构在至少一个区域中包含至少一种生物活性物质释放调节剂，以及至少部分由本发明的片状或管状结构覆盖的植入物。本发明进一步提供了一种生产本发明片状或管状结构的方法和设备，最好是定制和/或优化本发明片状或管状结构的方法和设备。本发明的片状或管状结构可在手术室植入前进行定制/优化。
• Methods using pores
  申请人：ISIS INNOVATION LIMITED

  公开号：EP2272981A1

  公开（公告）日：2011-01-12

  The invention relates to a method of identifying an individual nucleotide, comprising (a) contacting the nucleotide with a transmembrane protein pore so that the nucleotide interacts with the pore and (b) measuring the current passing through the pore during the interaction and thereby determining the identity of the nucleotide. The invention also relates to a method of sequencing nucleic acid sequences and kits related thereto.

  本发明涉及一种识别单个核苷酸的方法，包括：(a)将核苷酸与跨膜蛋白孔接触，使核苷酸与孔相互作用；(b)测量相互作用期间通过孔的电流，从而确定核苷酸的身份。本发明还涉及一种核酸序列测序方法及相关试剂盒。
• Advanced drug development and manufacturing
  申请人：Los Alamos National Security, LLC

  公开号：EP2511844A2

  公开（公告）日：2012-10-17

  There is described an apparatus for measuring protein characteristics comprising an X-ray fluorescence (XRF) spectrometer comprising a source of polychromatic X-rays, an X-ray detector, a protein, a molecule that has been exposed to and at least weakly binds to the protein, a plurality of X-ray fluorescence signal data obtained by irradiating chemical elements in the protein and molecule with the polychromatic X-rays and a security system for maintaining records for the data from the plurality of X-ray fluorescence signal measurements. There is also described an x-ray microscope for measuring a sample.

  描述了一种测量蛋白质特性的仪器，该仪器包括一个 X 射线荧光 (XRF) 光谱仪，其中包括一个多色 X 射线源、一个 X 射线探测器、一个蛋白质、一个已暴露于该蛋白质并至少与该蛋白质弱结合的分子、通过用多色 X 射线照射蛋白质和分子中的化学元素而获得的多个 X 射线荧光信号数据，以及一个用于维护多个 X 射线荧光信号测量数据记录的安全系统。此外，还介绍了一种用于测量样品的 X 射线显微镜。
• Enzyme-pore constructs
  申请人：Oxford Nanopore Technologies Limited

  公开号：EP2682460A1

  公开（公告）日：2014-01-08

  The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.

  本发明涉及由跨膜蛋白孔亚基和核酸处理酶组成的构建体。孔亚基与酶共价连接，使亚基和酶都能保持其活性。这些构建体可用来产生附有核酸处理酶的跨膜蛋白孔。这种孔特别适用于核酸测序。酶处理核酸的方式使孔能通过随机感应检测其核苷酸成分。
• METHOD FOR PRODUCING POLYSACCHARIDE
  申请人：Sanyo Chemical Industries, Ltd.

  公开号：EP2719767A1

  公开（公告）日：2014-04-16

  The present invention aims to provide a method for producing a polysaccharide with high efficiency using a polysaccharide synthase. The present invention provides a method for producing a polysaccharide, including allowing polysaccharide synthase (B) to act on ribonucleoside diphosphate-monosaccharide (A) shown below to produce a polysaccharide, wherein in 10 to 100% of the duration in which (B) acts on (A), the concentration of ribonucleoside diphosphate in a reaction solution is lower than 100 times an inhibitory concentration IC50 described below against polysaccharide synthase (B). Inhibitory concentration IC50: a concentration of ribonucleoside diphosphate at which an enzyme activity of polysaccharide synthase (B) is reduced by half while under a condition where (B) has a concentration at which (B) acts on ribonucleoside diphosphate-monosaccharide (A), wherein ribonucleoside diphosphate-monosaccharide (A) is used as a substrate and ribonucleoside diphosphate is used as an inhibitor. Ribonucleoside diphosphate-monosaccharide (A): a sugar nucleotide in which a proton of at least one hydroxyl group of at least one monosaccharide (a) selected from the group consisting of triose (a-1), tetrose (a-2), pentose (a-3), hexose (a-4), heptose (a-5), and monosaccharide (a-6) described below is substituted with a functional group represented by any one of chemical formulae (1) to (5) below. Monosaccharide (a-6) : a monosaccharide selected from the group consisting of (a-1), (a-2), (a-3), (a-4), and (a-5), in which at least one member selected from the group consisting of a proton, a hydroxyl group, and a hydroxymethyl group of the monosaccharide is substituted with substituent (E) described below. Substituent (E) is at least one substituent selected from the group consisting of carboxyl, amino, N-acetylamino, sulfate, methylester, N-glycolyl, methyl, 1,2,3-trihydroxypropyl, phosphate, and 2-carboxy-2-hydroxyethyl groups.

  本发明旨在提供一种利用多糖合成酶高效生产多糖的方法。本发明提供了一种生产多糖的方法，包括让多糖合成酶（B）作用于下图所示的核糖核苷二磷酸-单糖（A）以生产多糖，其中在（B）作用于（A）的10至100%的持续时间内，反应溶液中核糖核苷二磷酸的浓度低于下述对多糖合成酶（B）的抑制浓度IC50的100倍。 抑制浓度 IC50：在(B)对核糖核苷二磷酸单糖(A)起作用的浓度条件下，多糖合成酶(B)的酶活性降低一半的核糖核苷二磷酸浓度，其中核糖核苷二磷酸单糖(A)用作底物，核糖核苷二磷酸用作抑制剂。 核糖核苷二磷酸-单糖 (A)：一种糖核苷酸，其中至少一种单糖 (a) 的至少一个羟基的质子被下述化学式 (1) 至 (5) 中任一个所代表的官能团取代，该单糖选自三糖 (a-1)、四糖 (a-2)、戊糖 (a-3)、己糖 (a-4)、庚糖 (a-5) 和下述单糖 (a-6)。 单糖(a-6)：选自(a-1)、(a-2)、(a-3)、(a-4)和(a-5)组成的组的单糖，其中至少一个选自质子、羟基和羟甲基组成的组的成员被下述取代基(E)取代。 取代基(E)是选自羧基、氨基、N-乙酰氨基、硫酸基、甲基酯、N-甘氨酰、甲基、1,2,3-三羟基丙基、磷酸基和 2-羧基-2-羟乙基组成的组中的至少一个取代基。