1-((2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-(1-phenyl-1H-pyrazol-4-yl)pyrimidine-2,4(1H,3H)-dione

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘧啶核苷
• 英文名称: 1-((2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-(1-phenyl-1H-pyrazol-4-yl)pyrimidine-2,4(1H,3H)-dione
• 英文别名: 1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-(1-phenylpyrazol-4-yl)pyrimidine-2,4-dione
• 化学式: C₁₈H₁₈N₄O₅
• 分子量: 370.365
• InChiKey: CXVGZEXMLZYHKQ-ARFHVFGLSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  0.1
• 重原子数：
  27
• 可旋转键数：
  4
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.28
• 拓扑面积：
  117
• 氢给体数：
  3
• 氢受体数：
  6

反应信息

• 作为产物：
  描述：

  (2-氧代-1-苯肼)乙酸 在 copper(ll) sulfate pentahydrate 、 三乙醇胺 、 ascorbic acid sodium salt 作用下， 以 水 、 乙酸酐 、 叔丁醇 为溶剂， 反应 19.0h， 生成 1-((2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-(1-phenyl-1H-pyrazol-4-yl)pyrimidine-2,4(1H,3H)-dione
  参考文献：
  名称：

  使用高通量免疫分析筛选发现化学选择性和生物相容性反应

  摘要：

  一种基于免疫分析的方法被用来筛选偶极子和亲偶极子的多种组合，使其具有化学选择性和生物相容性[3 + 2]环加成反应的能力。该方法满足了点击概念的大多数要求，并导致了铜催化反应的发现，该反应由亚砜和炔烃试剂生成吡唑。

  DOI：

  10.1002/anie.201305645

文献信息

• METHOD FOR PRODUCING PYRAZOLES, NOVEL PYRAZOLES AND APPLICATIONS THEREOF
  申请人：COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES

  公开号：US20150368295A1

  公开（公告）日：2015-12-24

  A method for preparing a pyrazole of formula (I) in which R,R′ and R″ have different meanings, characterised in that it involves reacting a sydnone of formula (II) in which R and R′ have the meanings already indicated, with an alkyne of formula (III) in which R″ has the meaning already indicated, in the presence of copper, to obtain a pyrazole compound of formula (I) that is then isolated and salified if desired.

  一种制备式（I）的吡唑的方法，其中R、R′和R″具有不同的含义，其特征在于它涉及将式（II）的噻唑啉（其中R和R′已经指示了含义）与式（III）的炔烃（其中R″已经指示了含义）在铜的存在下反应，以获得式（I）的吡唑化合物，然后如有需要进行分离和盐化。
• Increasing the Stability of DNA:RNA Duplexes by Introducing Stacking Phenyl-Substituted Pyrazole, Furan, and Triazole Moieties in the Major Groove
  作者：Mick Hornum、Pawan Kumar、Patricia Podsiadly、Poul Nielsen

  DOI：10.1021/acs.joc.5b01577

  日期：2015.10.2

  Consecutive incorporations of our previously published thymidine analogue, 5-(1-pheny1-1H-1,2,3-triazol-4-yl)-2'-deoxyuridine monomer W in oligonudeotides, has demonstrated significant duplex-stabilizing properties due to its efficient staking properties in the major groove of DNA:RNA duplexes. The corresponding 2'-deoxycytidine analogue is not as well-accommodated in duplexes, however, due to its clear preference for the ring-flipped coplanar conformation. In our present work, we have used oh initio calculations to design two new building blocks, 5-(5-phenylfuran-2-yl)-2'-deoxycytidine monomer Y and 5-(1-phenyl-1H-pyrazol-3-yl)-2'-deoxycytidine monomer Z, that emulate the conformation of W. These monomers were synthesized by Suzuki-Miyaura couplings, and the pyrazole moiety was obtained in a cycloaddition from N-phenylsydnone. We show that the novel analogues Y and Z engage in efficient stacking either with themselves or with W due to a better overlap of the aromatic moieties. Importantly, we demonstrate that this translates into very thermally stable DNA:RNA duplexes, thus making Y and especially Z good candidates for improving the binding affinities of oligonudeotide-based therapeutics. Since we now have both efficiently stacking T and C analogues in hand, any purine rich stretch can be effectively targeted using these simple analogues. Notably, we show that the introduction of the aromatic rings in the major groove does not significantly change the helical geometry.
• US9802980B2
  申请人：——

  公开号：US9802980B2

  公开（公告）日：2017-10-31
• [EN] METHOD FOR PRODUCING PYRAZOLES, NOVEL PYRAZOLES AND APPLICATIONS THEREOF<br/>[FR] PROCÉDÉ DE FABRICATION DE PYRAZOLES, NOUVEAUX PYRAZOLES ET LEURS APPLICATIONS
  申请人：COMMISSARIAT ENERGIE ATOMIQUE

  公开号：WO2014122407A2

  公开（公告）日：2014-08-14

  Procédé de préparation d'un pyrazole de formule (I): dans laquelle R,R' et R'' ont différentes significations, caractérisé en ce que l'on fait réagir une sydnone de formule (II): dans laquelle R et R' ont les significations déjà indiquées, avec un alcyne de formule (III): dans laquelle R'' a la signification déjà indiquée, en présence de cuivre, pour obtenir un composé pyrazole de formule (I) que l'on isole et salifie si désiré.
• Discovery of Chemoselective and Biocompatible Reactions Using a High-Throughput Immunoassay Screening
  作者：Sergii Kolodych、Evelia Rasolofonjatovo、Manon Chaumontet、Marie-Claire Nevers、Christophe Créminon、Frédéric Taran

  DOI：10.1002/anie.201305645

  日期：2013.11.11

  An immunoassay‐based method was used to screen numerous combinations of dipoles and dipolarophiles for their ability to undergo chemoselective and biocompatible [3+2] cycloaddition reactions. The approach fulfills most of the requirements of the click concept and led to the discovery of a copper‐catalyzed reaction that generates pyrazoles from sydnone and alkyne reagents.

  一种基于免疫分析的方法被用来筛选偶极子和亲偶极子的多种组合，使其具有化学选择性和生物相容性[3 + 2]环加成反应的能力。该方法满足了点击概念的大多数要求，并导致了铜催化反应的发现，该反应由亚砜和炔烃试剂生成吡唑。