4-[[(2,3-二氢-2,2-二甲基-7-苯并呋喃氧基)羰基]氨基]丁酸 | 223922-62-1

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 中文名称: 4-[[(2,3-二氢-2,2-二甲基-7-苯并呋喃氧基)羰基]氨基]丁酸
• 英文名称: 4-[[(2,3-dihydro-2,2-dimethyl-7-benzofuranyloxy)carbonyl]amino]butanoic acid
• 英文别名: 4-[[(2,3-Dihydro-2,2-dimethyl-7-benzofuranyloxy)carbonyl]-amino]butanoic acid；4-[(2,2-dimethyl-3H-1-benzofuran-7-yl)oxycarbonylamino]butanoic acid
• CAS: 223922-62-1
• 化学式: C₁₅H₁₉NO₅
• 分子量: 293.32
• InChiKey: KRLRNENWNQPSPG-UHFFFAOYSA-N

物化性质

• 沸点：
  464.3±45.0 °C(Predicted)
• 密度：
  1.229±0.06 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  2.2
• 重原子数：
  21
• 可旋转键数：
  6
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.47
• 拓扑面积：
  84.9
• 氢给体数：
  2
• 氢受体数：
  5

反应信息

• 作为产物：
  描述：

  呋喃酚 在 三乙胺 、 三氟乙酸 作用下， 以 1,4-二氧六环 、 二氯甲烷 、 水 为溶剂， 反应 5.17h， 生成 4-[[(2,3-二氢-2,2-二甲基-7-苯并呋喃氧基)羰基]氨基]丁酸
  参考文献：
  名称：

  一种克百威羧基化半抗原的合成方法

  摘要：

  本发明“一种克百威羧基化半抗原的合成方法”，涉及生物化工技术。本发明是新型的克百威羧基化半抗原合成方法，合成步骤简洁有效，得率高，避免了传统三光气合成方法中大量使用光气和甲苯的危害性。以呋喃酚为起始原料，通过酯化和胺解反应，引入一条含4个碳和羧基端的手臂链，形成羧基化半抗原。采用活性酯法制备克百威人工抗原，紫外光谱扫描和免疫动物实验表明本发明制备的抗原良好的免疫原性。本发明的半抗原及人工抗原可完全用于克百威免疫分析中，满足国内对克百威残留的检测需要，应用前景广阔。

  公开号：

  CN105399711B

文献信息

• Development of an Enzyme-linked Immuno-Sorbent Assay (ELISA) Method for Carbofuran Residues
  作者：Jinyi Yang、Hong Wang、Yueming Jiang、Yuanming Sun、Ke Pan、Hongtao Lei、Qing Wu、Yudong Shen、Zhili Xiao、Zhenlin Xu

  DOI：10.3390/molecules13040871

  日期：——

  The haptens 4-[[(2,3-dihydro-2,2-dimethyl-7-benzofuranyloxy)carbonyl]-amino]butanoic acid (BFNB) and 6-[((2,3-dihydro-2,2-dimethyl-7-benzofuranyloxy)-carbonylamino]hexanoic acid (BFNH) were synthesized and then used to develop a rapid,specific and sensitive ELISA method to determine residues of the pesticide carbofuran in avariety of matrices. A hybridoma cell line (5D3) producing anti-carbofuran monoclonalantibodies (MAbs) was also established. Based on the MAbs in combination with theheterologous hapten BFNH coupled to either horseradish peroxidase (HRP) or ovalbumin(OVA), four ELISAs (formats I-IV) for the quantification of carbofuran were developedand compared. Among them, the optimized format II (the conjugate-coated directcompetitive ELISA) showed the best characteristics, with an IC50 value of 18.49 ng/mL, alimit of detection of 0.11 ng/mL and the shortest assay time (1 h). This ELISA method wasthen applied to the determinations of carbofuran in environmental water, soil and foodsamples. The relative standard deviations (R.S.D.s) ranged from 1.8% to 21.3% and themean recoveries were 104.6%, 108.3%, 106.3% and 100.1% for water, soil, lettuce andcabbage, respectively. Thus, the ELISA method of format II exhibited the potential todevelop commercial ELISA kits for a rapid detection of carbofuran for human health andenvironmental safety.

  半抗原 4-[[(2,3-二氢-2,2-二甲基-7-苯并呋喃氧基)羰基]-氨基]丁酸 (BFNB) 和 6-[((2,3-二氢-2,2-二甲基)合成了-7-苯并呋喃基氧基)-羰基氨基]己酸(BFNH)，然后用于开发快速、特异且灵敏的ELISA方法来测定各种产生抗-7-苯并呋喃基氧基)-羰基氨基]己酸的杂交瘤细胞系(5D3)中农药呋喃丹的残留量。还建立了呋喃丹单克隆抗体 (MAb)，基于该 MAb 与偶联辣根过氧化物酶 (HRP) 或卵清蛋白 (OVA) 的异源半抗原 BFNH，开发并比较了四种用于定量呋喃丹的 ELISA（格式 I-IV）。其中，优化的形式II（缀合物包被的直接竞争ELISA）表现出最好的特性，其IC50值为18.49 ng/mL，检测限为0.11 ng/mL，并且该ELISA的检测时间最短（1 h）。然后将该方法应用于环境水、土壤和食品样品中克百威的测定。水、土壤、生菜和卷心菜的相对标准偏差 (R.S.D.s) 范围为 1.8% 至 21.3%，平均回收率分别为 104.6%、108.3%、106.3% 和 100.1%。因此，格式II的ELISA方法展现了开发商业ELISA试剂盒的潜力，用于快速检测呋喃丹，以保障人类健康和环境安全。
• [EN] NANOBODY AGAINST CARBOFURAN PESTICIDE, PREPARATION METHOD THEREFOR AND APPLICATION THEREOF<br/>[FR] NANOCORPS DIRIGÉ CONTRE UN PESTICIDE DE CARBOFURAN, PROCÉDÉ DE PRÉPARATION CORRESPONDANT ET UTILISATION ASSOCIÉE<br/>[ZH] 一种克百威农药的纳米抗体及其制备方法和应用
  申请人：UNIV SOUTH CHINA AGRICULT

  公开号：WO2020215727A1

  公开（公告）日：2020-10-29

  一种克百威农药的纳米抗体及其制备方法和应用，该纳米抗体，其氨基酸序列如SEQ ID NO.1所示；编码其的基因，其核苷酸序列如SEQ ID NO.2所示。一种针对克百威农药的纳米抗体，该纳米抗体能够检测克百威农药，且检测结果准确、效果好、稳定性好，在高温和有机溶剂的条件下还有很好的检测效果。该抗体不仅可以广泛的应用于农产品中克百威农药残留的检测；还可以作为前体，通过随机或定点突变技术进行改造，能够获得性质(亲和性、特异性、稳定性等)更好的突变体，用来发展进一步用于食品、医药、农业等领域，有很大的应用推广价值。
• PHOSPHOLIPID ETHER (PLE) CAR T CELL TUMOR TARGETING (CTCT) AGENTS
  申请人：Seattle Children's Hospital (dba Seattle Children's Research Institute)

  公开号：US20200087399A1

  公开（公告）日：2020-03-19

  Aspects of the invention described herein relate to synthetic compounds that are useful for targeting and labeling tumor cells so as to facilitate recognition by binding agents including Chimeric Antigen Receptor T cells (CAR T cells), which are administered to a subject by intravenous or locoregional administration. Several compositions and methods of making and using these compositions to treat or inhibit a disease in a subject are contemplated.
• METHODS AND COMPOSITIONS FOR STIMULATION OF CHIMERIC ANTIGEN RECEPTOR T CELLS WITH HAPTEN LABELLED CELLS
  申请人：Seattle Children's Hospital (dba Seattle Children's Research Institute)

  公开号：US20210317407A1

  公开（公告）日：2021-10-14

  Some embodiments of the methods and compositions provided herein relate to the use of hapten labeled cells to stimulate chimeric antigen receptor (CAR) T cells. In some embodiments, CAR T cells can include a CAR that specifically binds to a hapten. Some embodiments relate to the in vivo or in vitro stimulation CAR T cells by hapten labeled cells.
• [EN] PHOSPHOLIPID ETHER (PLE) CAR T CELL TUMOR TARGETING (CTCT) AGENTS<br/>[FR] AGENTS DE CIBLAGE DE TUMEUR À LYMPHOCYTES T CAR (CTCT) D'ÉTHER DE PHOSPHOLIPIDE (PLE)
  申请人：SEATTLE CHILDRENS HOSPITAL DBA SEATTLE CHILDRENS RES INST

  公开号：WO2018148224A1

  公开（公告）日：2018-08-16

  Aspects of the invention described herein relate to synthetic compounds that are useful for targeting and labeling tumor cells so as to facilitate recognition by binding agents including Chimeric Antigen Receptor T cells (CAR T cells), which are administered to a subject by intravenous or locoregional administration. Several compositions and methods of making and using these compositions to treat or inhibit a disease in a subject are contemplated.