either glyoxal or methylglyoxal were used to further elucidate the underlying mechanisms of the N6-carboxymethyl lysine and N6-carboxyethyl lysine reaction cascades. After independent synthesis of the authentic reference standards, we were able to detect N6-glyoxylyl lysine and N6-pyruvoyl lysine for the first time by HPLC-MS2 analyses. These two novel amide advanced-glycation endproducts were exclusively
高反应性的α-二羰基化合物
乙二醛和
甲基乙二醛是体内翻译后蛋白质修饰的主要前体。的模型孵育Ñ 2 -吨-Boc赖
氨酸和任一
乙二醛或
甲基乙二醛被用来进一步阐明的基本机制Ñ 6 -carboxymethyl赖
氨酸和Ñ 6羧乙基赖
氨酸反应级联。的可信参考标准独立合成后,我们能够检测Ñ 6 -glyoxylyl赖
氨酸和Ñ 6通过HPLC-MS -pyruvoyl赖
氨酸首次2分析。这两种新的酰胺高级糖化终产物仅在充气条件下形成,表明它们是氧化应激的有效标志物。类似于众所周知的从
氨基酸到Strecker酸的Strecker降解途径,烯胺中间体的氧化被认为是关键的机械步骤。开发了一种用于测定组织中AGEs的高度灵敏的检测方法。为了支持我们的假设,
水平ñ 6 -glyoxylyl赖
氨酸和ñ 6 -pyruvoyl赖
氨酸在大鼠肝脏确实有肝硬化和老化有关。