3-azidopropanoyl CoA | 1437782-80-3

• 分子结构分类: 有机化合物 - 脂质和类脂质分子 - 脂肪酰基
• 英文名称: 3-azidopropanoyl CoA
• CAS: 1437782-80-3
• 化学式: C₂₄H₃₉N₁₀O₁₇P₃S
• 分子量: 864.618
• InChiKey: CUWFDOSQCQJWKZ-IEXPHMLFSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -0.63
• 重原子数：
  55.0
• 可旋转键数：
  22.0
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.67
• 拓扑面积：
  412.39
• 氢给体数：
  9.0
• 氢受体数：
  20.0

反应信息

• 作为产物：
  描述：

  3-Azidopropanoyl 3-azidopropanoate 、 辅酶 A 在 盐酸 作用下， 以 水 、 乙腈 为溶剂， 生成 3-azidopropanoyl CoA
  参考文献：
  名称：

  Labeling Lysine Acetyltransferase Substrates with Engineered Enzymes and Functionalized Cofactor Surrogates

  摘要：

  Elucidating biological and pathological functions of protein lysine acetyltransferases (KATs) greatly depends on the knowledge of the dynamic and, spatial localization of their enzymatic targets in the cellular proteome. We report the design and application of chemical probes for facile labeling and detection of substrates of the three major human KAT enzymes. In this approach, we create engineered KATs in junction with synthetic Ac-CoA surrogates to effectively label KAT, substrates even in the presence of competitive nascent cofactor acetyl-CoA. The functionalized and transferable acyl moiety of the Ac-CoA analogs further allowed the labeled substrates to be probed with alkynyl or azido-tagged fluorescent reporters by the copper-catalyzed azide-alkyne cycloaddition. The synthetic cofactors, in combination with either native or rationally engineered KAT enzymes, provide a versatile chemical biology strategy to label and profile cellular targets of KATs at the proteomic level.

  DOI：

  10.1021/ja311636b