The reduction of 17-oxosteroids to 17beta-hydroxysteroids is one of the important transformations for the preparation of many steroidal drugs and intermediates. The strain Zygowilliopsis sp. WY7905 was found to catalyze the reduction of C-17 carbonyl group of androst-4-ene-3,17-dione (AD) to give testosterone (TS) as the sole product by the constitutive 17beta-hydroxysteroid dehydrogenase (17beta-HSD)
17-氧甾体向17β-羟基甾体的还原是制备许多甾体药物和中间体的重要转变之一。菌株Zygowilliopsis sp。发现WY7905通过组成型17β-羟基类
固醇脱氢酶(17beta-H
SD)催化
雄酮-4-烯-3,17-二酮(AD)的C-17羰基还原,从而产生
睾丸激素(
TS)作为唯一产物。 。还原的最佳条件是pH 8.0和30摄氏度,补充10g / l
葡萄糖和1%Tween 80(w / v)。在优化的转化条件下,将0.75g / l的AD还原为单一产物
TS,其在24小时内的收率> 90%,非对映异构体过量(de)超过99%。该菌株还还原了其他17种氧类
固醇,例如
雌酮,3beta-hydroxyandrost-5-en-17-one和norandrostenedione,得到相应的17β-羟基类
固醇,而C-3和C-20羰基完好无损。在这种微
生物17β还原中不存在副产物将有助于产物纯化。这样