(2-amino-5-methylphenyl)phosphonic acid | 69675-98-5

• 分子结构分类: 有机化合物 - 苯类化合物 - 苯和取代衍生物
• 英文名称: (2-amino-5-methylphenyl)phosphonic acid
• 英文别名: 5-methyl-2-aminobenzenephosphonic acid；2-Amino-5-methyl-phenylphosphonsaeure
• CAS: 69675-98-5
• 化学式: C₇H₁₀NO₃P
• 分子量: 187.135
• InChiKey: WFEPUKFABWDABK-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  0.2
• 重原子数：
  12
• 可旋转键数：
  1
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.14
• 拓扑面积：
  83.6
• 氢给体数：
  3
• 氢受体数：
  4

反应信息

• 作为产物：
  描述：

  2-amino-5-methyl-phenylphosphonic acid diethylester 在 三甲基溴硅烷 作用下， 以 乙腈 为溶剂， 反应 16.0h， 以55%的产率得到(2-amino-5-methylphenyl)phosphonic acid
  参考文献：
  名称：

  [EN] METHODS AND COMPOSITIONS FOR THE REMOVAL OF ALDEHYDE ADDUCTS AND CROSSLINKS FROM BIOMOLECULES
  [FR] PROCÉDÉS ET COMPOSITIONS POUR L'ÉLIMINATION DE PRODUITS D'ADDITION ET DE RÉTICULATION D'ALDÉHYDE DE MOLÉCULES BIOLOGIQUES

  摘要：

  本发明提供了减少固定生物分子中醛基加合物和/或交联物数量的方法。在某些情况下，所述方法包括将含有醛固定生物分子（例如生物样品，如福尔马林固定石蜡嵌入（FFPE）组织样品）的样品与加合物逆转剂接触，所述逆转剂的用量和时间足以减少样品中醛固定相关的加合物和/或交联物的数量。在某些情况下，所述加合物逆转剂是一种化合物，其包括芳香环和至少一个以下物质之一：胺基和质子供体基团。在某些情况下，所述加合物逆转剂是从表1中选择的化合物之一。还提供了用于实施所述方法的组合物和试剂盒。

  公开号：

  WO2016044313A1
• 作为试剂：
  描述：

  苯甲醛 、 4-肼基-7-硝基-2,1,3-苯并氧杂恶二唑 在 (2-amino-5-methylphenyl)phosphonic acid 作用下， 以 aq. phosphate buffer 、 N,N-二甲基甲酰胺 为溶剂， 以17%的产率得到7-(2-benzylidenehydrazinyl)-4-nitrobenzo[2,1,3-d]oxadiazole
  参考文献：
  名称：

  Importance of ortho Proton Donors in Catalysis of Hydrazone Formation

  摘要：

  Anthranilic acids were recently reported as superior catalysts for hydrazone and oxime formation compared to aniline, the classic catalyst for these reactions. Here, alternative proton donors were examined with varied pK(a) in an effort to enhance activity at biological pH. The experiments show that 2-aminobenzenephosphonic acids are superior to anthranilic acids in catalyzing hydrazone formation with common aldehyde substrates.

  DOI：

  10.1021/ol400427x

文献信息

• COMPOSITIONS AND METHODS FOR USING FIXED BIOLOGICAL SAMPLES IN PARTITION-BASED ASSAYS
  申请人：10X Genomics, Inc.

  公开号：US20210190770A1

  公开（公告）日：2021-06-24

  The present disclosure provides compositions and methods for using fixed biological samples in partition-based assays. In at least one embodiment, the disclosure provides a composition comprising a fixed biological sample and an un-fixing agent contained in a partition, such as a discrete droplet. In some embodiments, the disclosure provides un-fixing agent compounds capable of catalytically cleaving crosslinks in fixed biological samples, particularly crosslinked nucleic acids, such as RNA.

  本公开提供了使用固定生物样本进行基于分区的检测的组合物和方法。在至少一个实施例中，本公开提供了一种组合物，包括一个固定的生物样本和一个包含在分区中的非固定剂，例如离散的液滴。在某些实施例中，本公开提供了能够催化断裂固定生物样本中的交联，特别是交联核酸（如RNA）的非固定剂化合物。
• METHODS AND COMPOSITIONS FOR THE REMOVAL OF ALDEHYDE ADDUCTS AND CROSSLINKS FROM BIOMOLECULES
  申请人：The Board of Trustees of the Leland Stanford Junio University

  公开号：US20170283860A1

  公开（公告）日：2017-10-05

  Methods are provided for reducing the number of aldehyde adducts and/or crosslinks from fixed biomolecules. In some cases, subject methods include contacting a sample having aldehyde fixed biomolecules (e.g., a biological sample such as a formalin fixed paraffin embedded (FFPE) tissue sample) with an adduct reversal agent in an amount and for a period of time sufficient to reduce the number of aldehyde fixation related adducts and/or crosslinks in the sample. In some cases, the adduct reversal agent is a compound that includes an aromatic ring and at least one of: an amine group and a proton-donating group. In some cases, the adduct reversal agent is a compound selected from the compounds of Table 1. Compositions and kits for practicing the subject methods are also provided.
• BIOMOLECULE PROCESSING FROM FIXED BIOLOGICAL SAMPLES
  申请人：Cell Data Sciences, Inc.

  公开号：US20190135774A1

  公开（公告）日：2019-05-09

  Molecular characterization of disease has become the dominant trend in modern medicine, and it has recently become increasingly important to obtain qPCR, microarray, and next-generation sequencing (NGS) data for both research and clinical applications. Formalin-fixed, paraffin-embedded (“FFPE”) samples have become the standard way of storing clinical biopsies throughout the world. Unfortunately, the poor quality and quantity of nucleic acids obtained from such specimens has posed severe limitations on the types of studies that can be accomplished. Existing methods for retrieval of the biomolecules from their crosslinked matrices are poorly effective and rely on harsh conditions which further damage the biomolecules being extracted. The invention provides compositions and methods for retrieval, analysis and use of biomolecules, including nucleic acids, from such samples.
• [EN] BIOMOLECULE PROCESSING FROM FIXED BIOLOGICAL SAMPLES<br/>[FR] TRAITEMENT DE BIOMOLÉCULES PROVENANT D'ÉCHANTILLONS BIOLOGIQUES FIXES
  申请人：CELL DATA SCIENCES INC

  公开号：WO2017184984A1

  公开（公告）日：2017-10-26

  Molecular characterization of disease has become the dominant trend in modern medicine, and it has recently become increasingly important to obtain qPCR, microarray, and next-generation sequencing (NGS) data for both research and clinical applications. Formalin-fixed, paraffin-embedded ("FFPE") samples have become the standard way of storing clinical biopsies throughout the world. Unfortunately, the poor quality and quantity of nucleic acids obtained from such specimens has posed severe limitations on the types of studies that can be accomplished. Existing methods for retrieval of the biomolecules from their crosslinked matrices are poorly effective and rely on harsh conditions which further damage the biomolecules being extracted. The invention provides compositions and methods for retrieval, analysis and use of biomolecules, including nucleic acids, from such samples.
• [EN] COMPOSITIONS AND METHODS FOR USING FIXED BIOLOGICAL SAMPLES IN PARTITION-BASED ASSAYS<br/>[FR] COMPOSITIONS ET MÉTHODES D'UTILISATION D'ÉCHANTILLONS BIOLOGIQUES FIXÉS DANS DES DOSAGES BASÉS SUR DES COMPARTIMENTS
  申请人：10X GENOMICS INC

  公开号：WO2021133842A1

  公开（公告）日：2021-07-01

  The present disclosure provides compositions and methods for using fixed biological samples in partition-based assays. In at least one embodiment, the disclosure provides a composition comprising a fixed biological sample and an un-fixing agent contained in a partition, such as a discrete droplet. In some embodiments, the disclosure provides un-fixing agent compounds capable of catalytically cleaving crosslinks in fixed biological samples, particularly crosslinked nucleic acids, such as RNA.