Boc-Leu-Gly-Arg-pNA

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 英文名称: Boc-Leu-Gly-Arg-pNA
• 英文别名: Boc-leucyl-L-glycyl-L-arginine-p-nitroaniline；Endotoxin substrate；tert-butyl N-[(2S)-1-[[2-[[(2S)-5-(diaminomethylideneamino)-1-(4-nitroanilino)-1-oxopentan-2-yl]amino]-2-oxoethyl]amino]-4-methyl-1-oxopentan-2-yl]carbamate
• 化学式: C₂₅H₄₀N₈O₇
• 分子量: 564.642
• InChiKey: XYZOMPCEZFRTOR-OALUTQOASA-N

计算性质

• 辛醇/水分配系数(LogP)：
  1.3
• 重原子数：
  40
• 可旋转键数：
  15
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.56
• 拓扑面积：
  236
• 氢给体数：
  6
• 氢受体数：
  8

反应信息

• 作为产物：
  描述：

  N-Boc-L-精氨酸 在 盐酸 、 N-羟基丁二酰亚胺 、 六氯环三磷腈 、 盐酸-N-乙基-Nˊ-(3-二甲氨基丙基)碳二亚胺 、 N,N-二异丙基乙胺 作用下， 以 乙醇 、 二氯甲烷 、 水 为溶剂， 反应 40.58h， 生成 Boc-Leu-Gly-Arg-pNA
  参考文献：
  名称：

  CN115974752

  摘要：

  公开号：

文献信息

• SUBSTRATE FOR ASSAYING BETA-GLUCAN AND/OR ENDOTOXIN AND ASSAY METHOD
  申请人：Kitagawa Takeshi

  公开号：US20100330700A1

  公开（公告）日：2010-12-30

  An object of the present invention is to provide a peptide derivative for determining β-glucan or endotoxin which allows high sensitivity measurement, and a method for determining β-glucan and/or endotoxin using the same. The present invention relates to (1) a peptide derivative represented by the following general formula [1]: X-A 1 -Gly-Arg-A 2 -E-D  [1], (2) a reagent for determining β-glucan and/or endotoxin comprising the above-described peptide derivative, (3) a method for determining β-glucan and/or endotoxin, characterized in that a sample containing β-glucan and/or endotoxin, an amebocyte lysate of a horseshoe crab and the above-described peptide derivative are reacted each other, then the resulting released compound represented by the following general formula [2]: H-A 2 -E-D  [2] is separated from unreacted substance and quantified, and the determination is made based on this value, and (4) a reagent kit for determining β-glucan and/or endotoxin, comprising an amebocyte lysate of a horseshoe crab and the above-described peptide derivative as constituents thereof.

  本发明的目的是提供一种用于测定β-葡聚糖或内毒素的肽衍生物，该肽衍生物允许高灵敏度测量，并提供使用该肽衍生物测定β-葡聚糖和/或内毒素的方法。本发明涉及(1)由以下通式[1]表示的肽衍生物：X-A1-Gly-Arg-A2-E-D[1]，(2)用上述肽衍生物的试剂用于测定β-葡聚糖和/或内毒素，(3)一种用于测定β-葡聚糖和/或内毒素的方法，其特征在于将含有β-葡聚糖和/或内毒素的样品、一种马蹄蟹的阿米巴溶解液和上述肽衍生物反应，然后分离出代表以下通式[2]的所释放的化合物：H-A2-E-D[2]，并对此值进行定量测定，然后进行测定，以及(4)用于测定β-葡聚糖和/或内毒素的试剂盒，其包括一种马蹄蟹的阿米巴溶解液和上述肽衍生物作为其组分。
• LIMULUS AMOEBOCYTE LYSATE G-FACTOR ACTIVATION INHIBITOR
  申请人：SEIKAGAKU KOGYO CO., LTD.

  公开号：EP0397880A1

  公开（公告）日：1990-11-22

  This invention relates to a limulus amoebocyte lysate G-factor activation inhibitor which contains as an active ingredient a polyglycoside having at least one poly-(1 3)-B-D-glucoside structure comprising 2 to 370 consecutive (1 3)-B-D-glucoside units represented by formula (I). This inhibitor is effective in inhibiting the activation of a G-factor which may be present in limulus amoebocyte lysate to be used in the Limulus test.

  本发明涉及一种嗜林变形虫细胞裂解液 G 因子活化抑制剂，它含有一种多糖苷作为有效成 分，该多糖苷至少具有一个由 2 至 370 个连续的 (1 3)-B-D-Glucoside 单元组成的多 (1 3)-B-D-Glucoside 结构，由式 (I) 表示。这种抑制剂能有效地抑制 G 因子的活化，这种 G 因子可能存在于 Limulus 变形细胞裂解液中，用于 Limulus 试验。
• ASSAYING AGENT FOR ENDOTOXIN
  申请人：SEIKAGAKU KOGYO KABUSHIKI KAISHA (SEIKAGAKU CORPORATION)

  公开号：EP0500947A1

  公开（公告）日：1992-09-02

  An assaying agent for endotoxin, which comprises either a limulus amoebocyte lysate and antibody against a (1 → 3)-β-D-glucan-sensitive factor or a limulus amoebocyte lysate which is substantially free from said factor. As this agent makes it possible to assay with high sensitivity and endotoxin originating in Gram-negative bacteria present in a biological specimen such as blood, urine or cerebrospinal fluid without being affected by (1 → 3)-β-D-glucan, it serves to diagnose gram-negative bacterial sepsis or other diseases which have been difficult to diagnose rapidly with good reproducibility, thus greatly contributing to clinical examination in particular.

  一种检测内毒素的试剂，它包括嗜膜变形虫细胞裂解液和针对（1→3）-β-D-葡聚糖敏感因子的抗体，或基本上不含上述因子的嗜膜变形虫细胞裂解液。由于这种制剂可以高灵敏度地检测血液、尿液或脑脊液等生物标本中存在的革兰氏阴性细菌产生的内毒素，而不受（1 → 3）-β-D-葡聚糖的影响，因此它可用于诊断革兰氏阴性细菌败血症或其他难以快速诊断的疾病，并具有良好的重现性，从而极大地促进了临床检查。
• PROCESS FOR PREPARING LIMULUS AMOEBOCYTE LYSATE
  申请人：SEIKAGAKU KOGYO KABUSHIKI KAISHA

  公开号：EP0507952A1

  公开（公告）日：1992-10-14

  A process for producing a limulus amoebocyte lysate which specifically reacts with endotoxin, which comprises bringing a limulus amoebocyte lysate into contact with an insoluble, immobilized (1→3)-β-D-glucoside structure to remove a (1→3)-β-D-glucan-sensitive factor (G factor) contained in the lysate. Since the resulting lysate is substantially free from the (1→3)-β-D-glucan-sensitive G factor, it specifically reacts with an endotoxin present in biological specimens such as blood, urine or spinal fluid to thereby allow the endotoxin to be assayed with a high sensitivity, thus contributing particularly to clinical examination.

  一种能与内毒素发生特异性反应的嗜酸性变形虫细胞裂解物的生产工艺，它包括使嗜酸性变形虫细胞裂解物与一种不溶解的固定化(1→3)-β-D-葡糖苷结构接触，以除去裂解物中所含的(1→3)-β-D-葡聚糖敏感因子(G因子)。由于得到的裂解物基本上不含 (1→3)-β-D 葡聚糖敏感 G 因子，因此它能与血液、尿液或脊髓液等生物标本中的内毒素发生特异性反应，从而能对内毒素进行高灵敏度检测，特别有助于临床检查。
• GEL PARTICLE MEASUREMENT REAGENT AND MEASUREMENT METHOD USING SAME
  申请人：Obata, Toru

  公开号：EP2631655A1

  公开（公告）日：2013-08-28

  Provided is a gel particle measurement reagent effective in quickly measuring a time point of initiation of production of gel particles. A gel particle measurement reagent R is a gel particle measurement reagent to be used to be agitated continuously with a sample S containing a target substance St as a measuring object to turn the target substance St into gel particles, including: a reagent base material 1 that undergoes a gelation reaction with the target substance St; and a biologically inactive particle formation accelerating factor 2 that is added to the reagent base material, has solubility in the sample S and dissolves therein at a concentration of 0.002 to 1%, and accelerates production of gel particles G whose particle sizes are centered in a predetermined range.

  本发明提供了一种凝胶粒子测量试剂，可有效快速测量凝胶粒子开始生成的时间点。凝胶粒子测量试剂 R 是一种凝胶粒子测量试剂，用于与含有目标物质 St 的样品 S 作为测量对象连续搅拌，以将目标物质 St 转化为凝胶粒子，包括：与目标物质 St 发生凝胶化反应的试剂基料 1；以及添加到试剂基料中、在样品 S 中具有溶解性并以 0.002至1%的浓度溶解其中，并加速凝胶颗粒G的生成，其颗粒大小以预定范围为中心。