2-(3,5-Diphenyltetrazol-2-ium-2-yl)-1,3-benzothiazole

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 唑类
• 英文名称: 2-(3,5-Diphenyltetrazol-2-ium-2-yl)-1,3-benzothiazole
• 化学式: C20H14N5S+
• 分子量: 356.4
• InChiKey: DWDVLDVLHQHOPL-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  7.2
• 重原子数：
  26
• 可旋转键数：
  3
• 环数：
  5.0
• sp3杂化的碳原子比例：
  0.0
• 拓扑面积：
  75.7
• 氢给体数：
  0
• 氢受体数：
  4

文献信息

• Diagnostics based on tetrazolium compounds
  申请人：LIFESCAN, INC.

  公开号：EP0990705A1

  公开（公告）日：2000-04-05

  A reagent is suitable for measuring the concentration of an analyte in a hemoglobin-containing biological fluid, such as whole blood. The reagent comprises dehydrogenase enzyme that has specificity for the analyte, NAD or an NAD derivative, a tetrazolium dye precursor, a diaphorase enzyme or an analog thereof, and a nitrite salt. The reagent causes dye formation that is a measure of the analyte concentration. The nitrite salt suppresses interfering dye formation caused non-enzymatically by the hemoglobin. Preferably, the reagent is used in a dry strip for measuring ketone bodies, such as beta-hydroxybutyrate.

  一种试剂适用于测量含血红蛋白生物液体（如全血）中分析物的浓度。试剂包括对分析物具有特异性的脱氢酶、NAD 或 NAD 衍生物、四唑染料前体、二磷酸盐酶或其类似物以及亚硝酸盐。试剂会导致染料形成，从而测量被分析物的浓度。亚硝酸盐可抑制血红蛋白非酶促地形成干扰染料。该试剂最好用于测量酮体（如β-羟基丁酸）的干条中。
• Stabilized tetrazolium-phenazine reagent compositions and methods for using the same
  申请人：Lifescan, Inc.

  公开号：EP1312921A1

  公开（公告）日：2003-05-21

  Stabilized tetrazolium dye-phenazine reagent compositions and methods for their use in the measurement of an analyte in a sample are provided. The subject reagent compositions include: (1) a tetrazolium dye component, e.g., a water soluble tetrazolium salt; (2) a phenazine component; and (3) an effective amount of one or more tetrazolium dye-phenazine stabilizing reagents, e.g., an inorganic Group IIIA compound and/or a flavin. In many embodiments, the subject reagent compositions include additional members of an analyte oxidizing signal producing system, such as: an analyte oxidizing enzyme, e.g., an analyte dehydrogenase or an analyte oxidase; and an enzyme cofactor. Also provided are test strips that include the subject reagent compositions, as well as systems and kits incorporating the subject test strips. The subject reagent compositions, test strips, systems and kits find use in the detection of a wide variety of analytes in a sample, such as a physiological sample, e.g., blood or a fraction thereof, or ISF (interstitial fluid).

  本发明提供了稳定四氮唑染料-吩嗪试剂组合物及其用于测量样品中分析物的方法。试剂组合物包括(1)四唑鎓染料成分，例如水溶性四唑鎓盐；(2)酚嗪成分；和(3)有效量的一种或多种四唑鎓染料-酚嗪稳定试剂，例如无机 IIIA 族化合物和/或黄素。在许多实施方案中，所述试剂组合物包括分析物氧化信号产生系统的其他成员，例如：分析物氧化酶，如分析物脱氢酶或分析物氧化酶；以及酶辅助因子。此外，还提供了包括主题试剂组合物的试纸，以及包含主题试纸的系统和试剂盒。这些试剂组合物、试纸条、系统和试剂盒可用于检测样品中的各种分析物，如生理样品，如血液或其一部分，或间质液（ISF）。
• Stabilized tetrazolium phenazine reagent compositions and methods for using the same
  申请人：——

  公开号：US20030096389A1

  公开（公告）日：2003-05-22

  Stabilized tetrazolium dye-phenazine reagent compositions and methods for their use in the measurement of an analyte in a sample are provided. The subject reagent compositions include: (1) a tetrazolium dye component, e.g., a water soluble tetrazolium salt; (2) a phenazine component; and (3) an effective amount of one or more tetrazolium dye-phenazine stabilizing reagents, e.g., an inorganic Group IIIA compound and/or a flavin. In many embodiments, the subject reagent compositions include additional members of an analyte oxidizing signal producing system, such as: an analyte oxidizing enzyme, e.g., an analyte dehydrogenase or an analyte oxidase; and an enzyme cofactor. Also provided are test strips that include the subject reagent compositions, as well as systems and kits incorporating the subject test strips. The subject reagent compositions, test strips, systems and kits find use in the detection of a wide variety of analytes in a sample, such as a physiological sample, e.g., blood or a fraction thereof, or ISF (interstitial fluid).

  本发明提供了稳定四氮唑染料-吩嗪试剂组合物及其用于测量样品中分析物的方法。试剂组合物包括(1)四唑鎓染料成分，例如水溶性四唑鎓盐；(2)酚嗪成分；和(3)有效量的一种或多种四唑鎓染料-酚嗪稳定试剂，例如无机 IIIA 族化合物和/或黄素。在许多实施方案中，所述试剂组合物包括分析物氧化信号产生系统的其他成员，例如：分析物氧化酶，如分析物脱氢酶或分析物氧化酶；以及酶辅助因子。此外，还提供了包括主题试剂组合物的试纸，以及包含主题试纸的系统和试剂盒。这些试剂组合物、试纸条、系统和试剂盒可用于检测样品中的各种分析物，如生理样品，如血液或其一部分，或间质液（ISF）。
• Stabilized tetrazolium reagent compositions and methods for using the same
  申请人：——

  公开号：US20030096332A1

  公开（公告）日：2003-05-22

  Stabilized tetrazolium dye reagent compositions and methods for their use in the measurement of an analyte in a sample are provided. The subject reagent compositions include a tetrazolium dye component, e.g., a water soluble tetrazolium salt, and an effective amount of a nitrite stabilizing agent, e.g., a nitrite salt. In many embodiments, the subject reagent compositions include additional members of an analyte oxidizing signal producing system, such as: an analyte oxidizing enzyme, e.g., an analyte dehydrogenase or an analyte oxidase; an electron transfer agent; and an enzyme cofactor. Also provided are test strips that include the subject reagent compositions, as well as systems and kits incorporating the subject test strips. The subject reagent compositions, test strips, systems and kits find use in the detection of a wide variety of analytes in a sample, such as a physiological sample, e.g., blood or a fraction thereof, or ISF (interstitial fluid).

  本发明提供了稳定的四唑鎓染料试剂组合物及其用于测量样品中分析物的方法。所述试剂组合物包括四唑染料成分，例如水溶性四唑盐，以及有效量的亚硝酸盐稳定剂，例如亚硝酸盐。在许多实施方案中，所述试剂组合物包括分析物氧化信号产生系统的其他成员，例如：分析物氧化酶，如分析物脱氢酶或分析物氧化酶；电子传递剂；以及酶辅助因子。此外，还提供了包括主题试剂组合物的试纸，以及包含主题试纸的系统和试剂盒。这些试剂组合物、试纸条、系统和试剂盒可用于检测样品中的各种分析物，如生理样品，如血液或其一部分，或间质液（ISF）。
• Methods, compositions and apparatuses for detection of gamma-hydroxybutyric acid (GHB)
  申请人：——

  公开号：US20030175846A1

  公开（公告）日：2003-09-18

  Methods, compositions and articles of manufacture for assaying a sample for a GHB source are provided. A sample suspected of containing a GHB source is contacted with a first oxidoreductase selective for GHB and an oxidized cofactor. In the presence of GHB in the sample, the first oxidoreductase oxidizes GHB to succinic semialdehyde and reduces the cofactor. The reduced cofactor thus produced can be detected directly, or a hydride abstractor can be used that abstracts a hydride from the reduced cofactor and produces a detectable change. The hydride abstractor can be a second oxidoreductase that oxidizes the reduced cofactor and produces a detectable change in a chromogen or dye. Preferably a visual change is produced, allowing performance of the assay outside of a laboratory setting. Fusion proteins comprising the first oxidoreductase, polynucleotides encoding such proteins, host cells expressing such proteins, and vectors comprising such polynucleotides are also provided. Stabilized formulations of the first oxidoreductase are also provided. Test supports, devices, and compositions and kits comprising reagents for performing such methods are also provided. Techniques for performing the assay in the presence of ethanol and in the presence of GHB precursors in the sample are also provided.

  本发明提供了用于化验 GHB 源的样品的方法、组合物和制造品。将怀疑含有伽马--羟丁酸源的样品与对伽马--羟丁酸有选择性的第一氧化还原酶和氧化辅助因子接触。当样品中存在伽马--羟丁酸时，第一氧化还原酶将伽马--羟丁酸氧化成琥珀酸半醛，并还原辅助因子。由此产生的还原型辅因子可以直接检测，也可以使用氢化物萃取剂，从还原型辅因子中萃取氢化物并产生可检测的变化。氢化物萃取剂可以是第二氧化还原酶，它可以氧化还原型辅因子，并在色原或染料中产生可检测的变化。最好能产生视觉变化，以便在实验室外进行检测。此外，还提供了包含第一氧化还原酶的融合蛋白、编码此类蛋白的多核苷酸、表达此类蛋白的宿主细胞以及包含此类多核苷酸的载体。还提供了第一氧化还原酶的稳定制剂。还提供了用于执行此类方法的测试支持物、装置以及包含试剂的组合物和试剂盒。还提供了在乙醇存在和样品中存在 GHB 前体的情况下进行检测的技术。