In one aspect, the present invention provides a method for preparing a sugar composition. The method includes: forming a mixture including polysaccharide biomass and an ionic liquid solution, wherein the ionic liquid solution contains water and an ionic liquid, and wherein the ionic liquid contains a dicarboxylic acid anion and a cation. The pH of the mixture is greater than or equal to about 10, and the molar ratio of the dicarboxylic acid anion to the cation is at least about 1:2. The method further includes: maintaining the mixture under conditions sufficient to dissolve at least a portion of the polysaccharide present in the polysaccharide biomass; reducing the pH of the mixture containing the dissolved polysaccharide to at least about 7; adding at least one glycoside hydrolase to the mixture having the reduced pH.
一方面,本发明提供了一种制备糖组合物的方法。该方法包括:形成包括
多糖生物质和
离子液体溶液的混合物,其中
离子液体溶液包含
水和
离子液体,
离子液体包含二
羧酸阴离子和阳离子。混合物的 pH 值大于或等于约 10,二
羧酸阴离子与阳离子的摩尔比至少约为 1:2。该方法进一步包括:将混合物保持在足以溶解
多糖生物质中存在的至少一部分
多糖的条件下;将含有溶解
多糖的混合物的pH值降低到至少约7;向pH值降低的混合物中加入至少一种糖苷
水解酶。