N-(3-Aminopropyl)-4-azido-2,3,5,6-tetrafluorobenzamide | 201464-12-2

• 分子结构分类: 有机化合物 - 苯类化合物 - 苯和取代衍生物
• 英文名称: N-(3-Aminopropyl)-4-azido-2,3,5,6-tetrafluorobenzamide
• 英文别名: N-(3-aminopropyl)-4-azido-2,3,5,6-tetrafluorobenzamide
• CAS: 201464-12-2
• 化学式: C₁₀H₉F₄N₅O
• 分子量: 291.208
• InChiKey: IMNHGKRUBPBBBN-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  2
• 重原子数：
  20
• 可旋转键数：
  5
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.3
• 拓扑面积：
  69.5
• 氢给体数：
  2
• 氢受体数：
  8

反应信息

• 作为反应物：
  描述：

  guanosine 5'-triphosphate sodium salt 、 N-(3-Aminopropyl)-4-azido-2,3,5,6-tetrafluorobenzamide 在 三苯基膦 作用下， 以 水 、 二甲基亚砜 为溶剂， 反应 1.42h， 以90%的产率得到
  参考文献：
  名称：

  基于 NTP γ-氨基磷酸酯的芳基叠氮化物衍生物的 RNA 聚合酶 II 起始底物的光活化类似物：官能团的合成和化学和光化学反应

  摘要：

  光活化衍生物 Ar-NH-(CH2)n-NHpppB（其中 Ar = 对叠氮基苯基 (A1)、5-叠氮基-2-硝基苯甲酰基 (A2) 或 4-叠氮基-2,3,5,6-四氟苯甲酰基 (A3) ) 组；B = Ado 或 Guo；n = 2、3 或 4) 被合成。发现氨基磷酸酯键的稳定性取决于杂环和可光活化基团的结构。A3、Ado 和 n = 3 的衍生物被水解，再生芳基叠氮化物和 ATP，而其他衍生物在水溶液中稳定。发现在特定转录起始和腺病毒晚期启动子控制的条件下，具有 A1 和 A2、B = Ado 和 n = 2 或 4 的光类似物表现为对来自酿酒酵母的 RNA 聚合酶 II 的起始底物。N-(4-叠氮基苯基)-1,4-二氨基丁烷和N-(5-叠氮基-2-硝基苯甲酰基)-1的光解，3-二氨基丙烷是光亲和试剂的两个功能片段，在水溶液中分别形成对苯醌二亚胺和对硝基-N-芳基羟胺衍生物。芳基羟胺

  DOI：

  10.1007/s11171-005-0046-0

文献信息

• CHEMICALLY SUBSTITUTED THERMOSENSITIVE PROBES AND COFACTORS FOR HOT START LIGATION
  申请人：TRILINK BIOTECHNOLOGIES

  公开号：US20140038181A1

  公开（公告）日：2014-02-06

  Provided herein are methods for ligase mediated nucleic acid replication and amplification of oligo- and probes containing substituted ligase components, particularly substituted ligase cofactors, substituted oligo- and probe acceptors, substituted oligo- and probe donors, substituted adenylated oligo- and polynucleotide donor intermediates carrying thermolabile group or groups. The substituted ligase components are not active until Hot Start activation step converts them into unsubstituted or natural ligase components, which fully support ligase reaction. The described methods are readily applied to ligation-based assays, especially utilizing Ligase Chain Reaction (LCR), for detection of a nucleic acid sequence where the use of the substituted ligase components improves an overall efficiency of LCR, increase discrimination between matched and mismatched templates and reduces or eliminates appearance of false positive signal. Furthermore, the use of the substituted ligase components reduces or eliminates the false positive signal originated from the template independent and blunt-ended ligation.

  本文提供了一种利用连接酶介导的核酸复制和扩增方法，其中包含了含有取代连接酶组分（特别是取代连接酶辅因子、取代寡核苷酸接受体、取代寡核苷酸供体和携带热敏基团的取代腺苷化寡核苷酸供体中间体）的寡核苷酸和探针的扩增。这些取代连接酶组分在热启动激活步骤之前是不活跃的，直到转化为非取代或自然连接酶组分，才能完全支持连接反应。所述方法可轻松应用于基于连接的检测，特别是利用连接链反应（LCR）检测核酸序列，其中使用取代连接酶组分可提高LCR的整体效率，增加匹配和不匹配模板之间的区别，并减少或消除假阳性信号的出现。此外，使用取代连接酶组分可减少或消除由模板非依赖性和钝端连接引起的假阳性信号。
• [EN] CHEMICALLY SUBSTITUTED THERMOSENSITIVE PROBES AND COFACTORS FOR HOT START LIGATION<br/>[FR] SONDES THERMOSENSIBLES CHIMIQUEMENT SUBSTITUÉES ET COFACTEURS POUR LIGATURE AVEC DÉPART À CHAUD
  申请人：TRILINK BIOTECHNOLOGIES

  公开号：WO2012094343A1

  公开（公告）日：2012-07-12

  Provided herein are methods for ligase mediated nucleic acid replication and amplification of oligo- and probes containing substituted ligase components, particularly substituted ligase cofactors, substituted oligo- and probe acceptors, substituted oligo- and probe donors, substituted adenylated oligo- and polynucleotide donor intermediates carrying thermolabile group or groups. The substituted ligase components are not active until Hot Start activation step converts them into unsubstituted or natural ligase components, which fully support ligase reaction. The described methods are readily applied to ligation-based assays, especially utilizing Ligase Chain Reaction (LCR), for detection of a nucleic acid sequence where the use of the substituted ligase components improves an overall efficiency of LCR, increase discrimination between matched and mismatched templates and reduces or eliminates appearance of false positive signal. Furthermore, the use of the substituted ligase components reduces or eliminates the false positive signal originated from the template independent and blunt-ended ligation.
• Photoactivatable Analogues of the Initiating Substrates of RNA Polymerase II Based on Aryl Azide Derivatives of NTP γ-Amidophosphate: Synthesis and Chemical and Photochemical Reactions of Functional Groups
  作者：D. G. Knorre、N. V. Kudryashova、T. V. Popova、M. M. Shakirov、V. S. Mal’shakova、O. E. Shpenev、L. K. Savinkova、M. V. Serebryakova、T. S. Godovikova

  DOI：10.1007/s11171-005-0046-0

  日期：2005.7

  reagents, in aqueous solutions was established to result in the formation of p-benzoquinone diimine and p-nitro-N-arylhydroxylamine derivatives, respectively. The arylhydroxylamine derivatives undergo a number of transformations in aqueous solution leading to nitroso derivatives. We concluded that it is this nitroso derivative (products of nitrene transformation, rather than the nitrenes themselves) that

  光活化衍生物 Ar-NH-(CH2)n-NHpppB（其中 Ar = 对叠氮基苯基 (A1)、5-叠氮基-2-硝基苯甲酰基 (A2) 或 4-叠氮基-2,3,5,6-四氟苯甲酰基 (A3) ) 组；B = Ado 或 Guo；n = 2、3 或 4) 被合成。发现氨基磷酸酯键的稳定性取决于杂环和可光活化基团的结构。A3、Ado 和 n = 3 的衍生物被水解，再生芳基叠氮化物和 ATP，而其他衍生物在水溶液中稳定。发现在特定转录起始和腺病毒晚期启动子控制的条件下，具有 A1 和 A2、B = Ado 和 n = 2 或 4 的光类似物表现为对来自酿酒酵母的 RNA 聚合酶 II 的起始底物。N-(4-叠氮基苯基)-1,4-二氨基丁烷和N-(5-叠氮基-2-硝基苯甲酰基)-1的光解，3-二氨基丙烷是光亲和试剂的两个功能片段，在水溶液中分别形成对苯醌二亚胺和对硝基-N-芳基羟胺衍生物。芳基羟胺