Ac-Lys-pTyr-Glu-Glu-Ile-Glu-Lys | 1187337-05-8

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 英文名称: Ac-Lys-pTyr-Glu-Glu-Ile-Glu-Lys
• 英文别名: Ac-KpYEEIEK
• CAS: 1187337-05-8
• 化学式: C₄₄H₇₀N₉O₁₉P
• 分子量: 1060.06
• InChiKey: KQMGRVWTFSSAQJ-ZOVYDMCFSA-N

反应信息

• 作为产物：
  描述：

  在 水 作用下， 以 N,N-二甲基甲酰胺 为溶剂， 反应 19.0h， 以38%的产率得到Ac-Lys-pTyr-Glu-Glu-Ile-Glu-Lys
  参考文献：
  名称：

  Synthesis and evaluation of phosphopeptides containing iminodiacetate groups as binding ligands of the Src SH2 domain

  摘要：

  Phosphopeptide pTyr-Glu-Glu-Ile (pYEEI) has been introduced as an optimal Src SH2 domain ligand. Peptides, Ac-K(IDA)pYEEIEK(IDA) (1), Ac-KpYEEIEK (2), Ac-K(IDA) pYEEIEK (3), and Ac-KpYEEIEK(IDA) (4), containing 0-2 iminodiacetate (IDA) groups at the N- and C-terminal lysine residues were synthesized and evaluated as the Src SH2 domain binding ligands. Fluorescence polarization assays showed that peptide 1 had a higher binding affinity (K-d = 0.6 mu M) to the Src SH2 domain when compared with Ac-pYEEI (K-d = 1.7 mu M), an optimal Src SH2 domain ligand, and peptides 2-4 (K-d = 2.9-52.7 mu M). The binding affinity of peptide 1 to the SH2 domain was reduced by more than 2-fold (K-d = 1.6 mu M) upon addition of Ni2+ (300 mu M), possibly due to modest structural effect of Ni2+ on the protein as shown by circular dichroism experimental results. The binding affinity of 1 was restored in the presence of EDTA (300 mu M) (K-d = 0.79 mu M). These studies suggest that peptides containing IDA groups may be used for designing novel SH2 domain binding ligands. (C) 2009 Elsevier Inc. All rights reserved.

  DOI：

  10.1016/j.bioorg.2009.05.003