FLAVONE-3-DIPHOSPHATE | 28464-44-0

• 分子结构分类: 有机化合物 - 苯丙烷和聚酮 - 黄酮类化合物
• 英文名称: FLAVONE-3-DIPHOSPHATE
• 英文别名: (4-Oxo-2-phenylchromen-3-yl) phosphono hydrogen phosphate
• CAS: 28464-44-0
• 化学式: C₁₅H₁₂O₉P₂
• 分子量: 398.202
• InChiKey: JWTIYHBWVKHAAG-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  1
• 重原子数：
  26
• 可旋转键数：
  5
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.0
• 拓扑面积：
  140
• 氢给体数：
  3
• 氢受体数：
  9

文献信息

• Complexing of biologically active or functional compounds and methods of preparing and utilizing same
  申请人：ENZO BIOCHEM, INC.

  公开号：EP0123300A2

  公开（公告）日：1984-10-31

  Complexes containing a biologically active or functional compound or compounds are prepared by attaching or fixing a first compound, which may be a biologically active or functional compound, to a solid substrate under conditions such that the active site of said first compound is protected, thereupon derivatizing or combining said first compound with a derivatizing or modifying compound while said first compound is attached to said solid substrate. The resulting complex or complexed first compound is then released from attachment to said solid substrate to yield the complex, said complex comprising said derivatizing compound attached to said first compound, said first compound, upon release from the solid substrate having available its functional or biologically active site. For example, alkaline phosphatase is attached to a solid substrate, such as dextran beads, preferably through a ligand, such as a competitive inhibitor for alkaline phosphatase, e.g. para-aminobenzyl-phosphonic acid (p-ABPA). The dextran beads would be first treated with (p-ABPA) and the alkaline phosphatase brought into contact with the thus-treated dextran beads. The competitive inhibitor p-ABPA serves as a ligand for attachment of the alkaline phosphatase to the dextran beads while at the same time occupying and protecting the active site of the alkaline phosphatase. Thereupon, the thus-attached alkaline phosphatase is derivatized or modified, such as by biotinylation. Upon biotinylation of the alkaline phosphatase, the resulting biotinylated alkaline phosphatase is eluted or removed from attachment to the p-ABPA treated solid substrate or dextran beads to which it was fixed, yielding a biotinylated alkaline phosphatase with the active or functional site of the alkaline phosphatase intact. The complex, while still attached to the dextran beads, could be further treated with a second compound or component which might also, if desired, provide an active or functional site, such as by contact with avidin, which would bind to the modifying or derivatizing moiety, i.e. biotin, of the biotinylated alkaline phosphatase and the thus-produced avidin-biotinylated alkaline phosphatase complex then eluted. This complex, such as a complex comprising avidin-biotinylated alkaline phosphatase, could be employed as a detector in a detection system for the identification and/or location of another compound, such as a biotinylated compound, e.g. a biotinylated nucleotide or biotinylated DNA.

  制备含有一种或多种生物活性或功能性化合物的复合物的方法是：在使所述第一种化合物的活性位点受到保护的条件下，将第一种化合物（可能是一种生物活性或功能性化合物）附着或固定在固体基质上，然后在所述第一种化合物附着在所述固体基质上的同时，将所述第一种化合物与一种衍生或修饰化合物衍生或结合。然后将得到的复合物或复配的第一化合物从附着在所述固体底物上释放出来，得到复合物，所述复合物包括附着在所述第一化合物上的所述衍生化合物，所述第一化合物从固体底物上释放出来后，其功能或生物活性位点可用。例如，碱性磷酸酶最好通过配体（如碱性磷酸酶的竞争性抑制剂，如对氨基苄基膦酸（p-ABPA））附着在固体基质（如葡聚糖珠）上。首先用（p-ABPA）处理葡聚糖珠，然后让碱性磷酸酶与这样处理过的葡聚糖珠接触。竞争性抑制剂 p-ABPA 可作为配体，使碱性磷酸酶附着在葡聚糖珠上，同时占据并保护碱性磷酸酶的活性位点。然后，对这样附着的碱性磷酸酶进行衍生化或修饰，如生物素化。在对碱性磷酸酶进行生物素化后，得到的生物素化碱性磷酸酶会被洗脱或去除附着在经 p-ABPA 处理过的固体底物或葡聚糖珠上的固定物，从而得到生物素化的碱性磷酸酶，而碱性磷酸酶的活性或功能位点完好无损。当该复合物仍附着在葡聚糖珠上时，可以用第二种化合物或成分进一步处理，如果需要，这种化合物或成分还可以提供一个活性或功能位点，例如通过与阿维丁接触，与生物素化碱性磷酸酶的修饰或衍生分子（即生物素）结合，然后洗脱由此产生的阿维丁-生物素化碱性磷酸酶复合物。这种复合物，如由阿维菌素-生物素化碱性磷酸酶组成的复合物，可用作检测系统中的检测器，用于识别和/或定位另一种化合物，如生物素化化合物，如生物素化核苷酸或生物素化 DNA。
• A method for detecting drug-induced thrombocytopenia and an immunoassay kit for use in the same
  申请人：AKZO N.V.

  公开号：EP0165681A2

  公开（公告）日：1985-12-27

  There is disclosed a method for detecting drug-induced thrombocytopenia, which comprises the steps of: (a) treating blood platelets with serum or plasma derived from a patient suspected of having a drug-induced thrombocytopenia and with a drug suspected of causing the thrombocytopenia; (b) incubating the treated platelets for from 15 to 25 minutes at a temperature of from 21 C to 24 C; (c) washing the incubated platelets with a wash solution containing the drug and then resuspending the platelets; (d) contacting, and incubating together, the resuspended platelets with a solid support having a specific binding protein bound thereto, (e) decanting unbound platelets; (f) measuring the bound platelets by: (i) contacting, and incubating, the solid support having platelets bound thereto and an enzyme conjugate; (ii) removing unbound material; (iii) contacting, and incubating together, the support-platelet-enzyme conjugate complex, and a substrate; and (iv) determining the quantity of substrate acted upon by the enzyme conjugate; and (g) comparing the determined quantity with at least one of a standard value and a value derived by subjecting a drug-free control to steps (a) to (f).

  本发明公开了一种检测药物诱发血小板减少症的方法，该方法包括以下步骤： (a) 用疑似药物诱发血小板减少症患者的血清或血浆以及疑似引起血小板减少症的药物处理血小板； (b) 将处理过的血小板在 21 摄氏度至 24 摄氏度的温度下培养 15 至 25 分钟； (c) 用含有药物的洗涤液洗涤培养的血小板，然后重新悬浮血小板； (d) 将重新悬浮的血小板与结合了特异性结合蛋白的固体支持物接触并一起孵育、 (e) 倾倒未结合的血小板； (f) 通过以下方法测量结合的血小板： (i) 将结合有血小板的固体支持物与酶结合物接触并孵育； (ii) 去除未结合的物质 (iii) 将支撑物-血小板-酶结合物和底物接触并一起孵育；以及 (iv) 确定酶结合物作用于底物的数量；以及 (g) 将确定的数量与标准值和将不含药物的对照物置于步骤(a)至(f)中得出的值中的至少一个进行比较。
• A method of selecting an antibody, a hybridoma, a monoclonal antibody and use thereof
  申请人：Takeda Chemical Industries, Ltd.

  公开号：EP1258729A2

  公开（公告）日：2002-11-20

  The present invention provides a method of selecting an antibody against a target substance to be measured which comprises selecting the antibody against the target substance by antigen-antibody reaction in the presence of a substance interfering with the antigen-antibody reaction. That is, an antigen and a labeled antigen are reacted with the antibody in the presence of an interfering substance such as an environment pollutant, and on the basis of the degree of reaction thereof, the antibody against the target substance highly resistant to the interfering substance is selected. Thereby, even if a test sample is contaminated with a substance interfering with antigen-antibody reaction, the antibody highly resistant to a substance interfering with antigen-antibody reaction is not influenced by the interfering substance and gives a correct value in the quantification.

  本发明提供了一种针对待测目标物质的抗体选择方法，该方法包括在干扰抗原-抗体反应的物质存在下，通过抗原-抗体反应选择针对目标物质的抗体。 也就是说，在干扰物质（如环境污染物）存在的情况下，抗原和标记抗原与抗体反应，根据反应程度选择对干扰物质具有高度抵抗力的目标物质抗体。这样，即使测试样品被干扰抗原-抗体反应的物质污染，对干扰抗原-抗体反应的物质具有高度抗性的抗体也不会受到干扰物质的影响，并能给出正确的定量值。
• oriC specific nucleic acid sequences and use thereof
  申请人：Ludwig-Maximilians-Universität München

  公开号：EP1767651A1

  公开（公告）日：2007-03-28

  The present invention is related to a combination of at least a first nucleic acid molecule and at least a second nucleic acid molecule for the detection of and/or discrimination between bacteria of the group of coliforms and bacteria of the Escherichia coli genogroup, whereby the first nucleic acid and the second nucleic acid differ in their nucleic acid sequence and comprise fragments of the origin of replication (oriC) locus sequence of said bacteria.

  本发明涉及至少一种第一核酸分子和至少一种第二核酸分子的组合，用于检测和/或区分大肠菌群细菌和大肠埃希氏菌基因组细菌，其中第一核酸和第二核酸的核酸序列不同，包含所述细菌的复制源（oriC）基因座序列片段。
• Method of selecting an antibody, a hybridoma, a monoclonal antibody and use thereof
  申请人：——

  公开号：US20030064389A1

  公开（公告）日：2003-04-03

  The present invention provides a method of selecting an antibody against a target substance to be measured which comprises selecting the antibody against the target substance by antigen-antibody reaction in the presence of a substance interfering with the antigen-antibody reaction. That is, an antigen and a labeled antigen are reacted with the antibody in the presence of an interfering substance such as an environment pollutant, and on the basis of the degree of reaction thereof, the antibody against the target substance highly resistant to the interfering substance is selected. Thereby, even if a test sample is contaminated with a substance interfering with antigen-antibody reaction, the antibody highly resistant to a substance interfering with antigen-antibody reaction is not influenced by the interfering substance and gives a correct value in the quantification.

  本发明提供了一种针对待测目标物质的抗体选择方法，该方法包括在干扰抗原-抗体反应的物质存在下，通过抗原-抗体反应选择针对目标物质的抗体。 也就是说，在干扰物质（如环境污染物）存在的情况下，抗原和标记抗原与抗体反应，根据反应程度选择对干扰物质具有高度抵抗力的目标物质抗体。这样，即使测试样品被干扰抗原-抗体反应的物质污染，对干扰抗原-抗体反应的物质具有高度抗性的抗体也不会受到干扰物质的影响，并能给出正确的定量值。