6-amino-1-(2-deoxy-β-D-erythro-pentofuranosyl)-1,5-dihydro-3-iodo-4H-pyrazolo[3,4-d]pyrimidin-4-one | 183274-53-5

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 吡唑并嘧啶类
• 英文名称: 6-amino-1-(2-deoxy-β-D-erythro-pentofuranosyl)-1,5-dihydro-3-iodo-4H-pyrazolo[3,4-d]pyrimidin-4-one
• 英文别名: 6-amino-1-(2-deoxy-β-D-erythro-pentofuranosyl)-1,5-dihydro-3-iodo-4H-pyrazolo<3,4-d>pyrimidin-4-one；7-iodo-8-aza-7-deaza-deoxyguanosine；7-iodo-7-deaza-8-aza-2'-deoxyguanosine；7-iodo-8-aza-7-deaza-2'-deoxyguanosine；6-amino-1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-3-iodo-5H-pyrazolo[3,4-d]pyrimidin-4-one
• CAS: 183274-53-5
• 化学式: C₁₀H₁₂IN₅O₄
• 分子量: 393.141
• InChiKey: LJLWCUMCUZGHMU-VPENINKCSA-N

物化性质

• 密度：
  2.69±0.1 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  -1.2
• 重原子数：
  20
• 可旋转键数：
  2
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.5
• 拓扑面积：
  135
• 氢给体数：
  4
• 氢受体数：
  6

反应信息

• 作为反应物：
  描述：

  6-amino-1-(2-deoxy-β-D-erythro-pentofuranosyl)-1,5-dihydro-3-iodo-4H-pyrazolo[3,4-d]pyrimidin-4-one 在 吡啶 、 六甲基二硅氮烷 作用下， 以 四氢呋喃 、 N,N-二甲基甲酰胺 为溶剂， 反应 17.5h， 生成 1-[2-deoxy-5-O-(4,4'-dimethoxytrityl)-β-D-erythro-pentofuranosyl]-1,5-dihydro-3-iodo-6-[(2-methylpropanoyl)amino]-4H-pyrazolo[3,4-d]pyrimidin-4-one 3'-(2-cyanoethyl diisopropylphosphoramidite)
  参考文献：
  名称：

  Stabilisation of duplex DNA by 7-halogenated 8-aza-7-deazaguanines

  摘要：

  含有 7 卤化 8-aza-7-deaza-2′-deoxyguanosine (c7z8Gd) 衍生物的寡核苷酸，如 d(Br7c7z8 G-C)4 8（Tm = 88 ℃）和 d(I7c7z8 G-C)4 9（Tm = 84 ℃），比 d(G-C)4 5（Tm = 59 ℃）稳定得多。

  DOI：

  10.1039/a804414g
• 作为产物：
  描述：

  (2R,3S,5R)-5-(6-amino-3-iodo-4-methoxypyrazolo[3,4-d]pyrimidin-1-yl)-2-(hydroxymethyl)oxolan-3-ol 在 sodium hydroxide 作用下， 以 1,4-二氧六环 为溶剂， 反应 5.0h， 以60.6%的产率得到6-amino-1-(2-deoxy-β-D-erythro-pentofuranosyl)-1,5-dihydro-3-iodo-4H-pyrazolo[3,4-d]pyrimidin-4-one
  参考文献：
  名称：

  氮杂化鸟嘌呤核苷及其合成方法和在DNA测序 中的用途

  摘要：

  本发明公开了一种氮杂化鸟嘌呤核苷及其合成方法和在DNA测序中的用途；所述合成方法包括如下步骤：式(Ⅲ)化合物在碱性条件下去保护基得式(Ⅱ)化合物；进一步去甲基得式(Ⅰ)化合物，即7‑去氮‑7‑卤素‑8‑氮‑鸟嘌呤核苷；其中，R1为H或OH，R2为I、Br或Cl，R3为H或本发明的氮杂化鸟嘌呤核苷是用于DNA测序的新型试剂，与8位未取代氮的鸟嘌呤核苷相比，具有更好的碱基识别效果、更稳定的DNA链结构。同时，现有技术中8位氮取代鸟嘌呤核苷的合成方法复杂，产率低，尚未商业化生产，而本发明的合成方法所需原料简单易得，采用常规化学合成反应，且收率较高，适用于广泛推广使用。

  公开号：

  CN103804447B

文献信息

• Synthesis of 7-Halogenated 8-Aza-7-deaza-2′-deoxyguanosines and Related Pyrazolo[3,4-d]pyrimidine 2′-Deoxyribonucleosides
  作者：Frank Seela、Georg Becher

  DOI：10.1055/s-1998-4483

  日期：1998.2

  The synthesis of 7-bromo and 7-iodo derivatives of 8-aza-7-deaza-2′-deoxyguanosine (2, 3) as well as the halogenated 4-alkoxy derivatives 4 a - c and 5 a - c is described. Glycosylation of the halogenated pyrazolo[3,4-d]pyrimidine anions of 7 a -c or 8 a - c with 2-deoxy-3,5-di-O-(p-toluoyl)-α-d-erythro-pentofuranosyl chloride (9) yields regioisomeric glycosylation products, the N(1)-isomers 10 a - c and 11 a - c as well as the N(2)-compounds 12 a - c. The latter isomers lose their halogen during the glycosylation in the presence of non-anhydrous KOH. Anhydrous conditions (NaH) furnished 10 c, 11 c together with the halogenated N(2)-isomers 13 a,b. Compounds 10 a - c, and 11 a - c were deprotected and converted to the 4-alkoxy nucleosides 4 a - c and 5 a - c. The N(1)-nucleosides 4 c and 5 c were hydrolyzed to give the 7-bromo or 7-iodo derivatives of 8-aza-7-deaza-2′-deoxyguanosines 2 and 3. Different from regular 2′-deoxyribonucleosides the sugar moiety of pyrazolo[3,4-d]pyrimidine 2′-deoxyribonucleosides shows a preferred N-type pucker (3T2) in solution, a conformation which is also detected in the solid state.

  描述了8-氮杂-7-脱氮-2′-去氧鸟苷（2, 3）的7-溴和7-碘衍生物的合成，以及卤化的4-烷氧基衍生物4a-c和5a-c。将7a-c或8a-c的卤化吡唑[3,4-d]嘧啶阴离子与2-去氧-3,5-二-O-(对甲苯酰基)-α-d-赤糖善氟糖氯化物（9）进行糖苷化反应，得到区域异构糖苷化产物，N(1)-异构体10a-c和11a-c，以及N(2)-化合物12a-c。后者异构体在无水氢氧化钾存在下糖苷化时失去卤素。在无水条件下（氢化钠）生成了10c、11c以及卤化的N(2)-异构体13a,b。化合物10a-c和11a-c被去保护并转化为4-烷氧基核糖苷4a-c和5a-c。N(1)-核糖苷4c和5c被水解，得到8-氮杂-7-脱氮-2′-去氧鸟苷的7-溴或7-碘衍生物2和3。与常规的2′-去氧核糖核苷不同，吡唑[3,4-d]嘧啶2′-去氧核糖核苷的糖部分在溶液中显示出优先的N型扭曲（3T2），这种构象在固态中也得到了验证。
• Breaking the conservation of guanine residues in the catalytic loop of 10–23 DNAzyme by position-specific nucleobase modifications for rate enhancement
  作者：Yang Liu、Zhiwen Li、Gaofeng Liu、Qi Wang、Wei Chen、Di Zhang、Maosheng Cheng、Zhibing Zheng、Keliang Liu、Junlin He

  DOI：10.1039/c3cc42067a

  日期：——

  10–23 DNAzyme has been used as a very good model for exploring the potential of functional nucleic acids. Its 15-mer catalytic core has been demonstrated to be the optimized in vitro selection result. However, with the introduction of protein-like functional groups through 2′-deoxyguanosine analogues, its two highly conserved guanine residues (G2 and G14) can be modified for more efficient 10–23 DNAzyme analogues. This result implies that chemical modifications of functional nucleic acids with well-designed nucleoside analogues of each canonical residue could be used as the first step in the efforts toward more powerful functions of nucleic acids.

  10â23 DNA 酶被用作探索功能核酸潜力的一个非常好的模型。它的 15 个聚合物催化核心已被证明是体外选择的最佳结果。然而，通过 2â²-脱氧鸟苷类似物引入类似蛋白质的功能基团，其两个高度保守的鸟嘌呤残基（G2 和 G14）可被修饰为更有效的 10â23 DNA 酶类似物。这一结果表明，用精心设计的核苷类似物对功能核酸进行化学修饰，可作为实现核酸更强大功能的第一步。
• Synthesis and ‘double click’ density functionalization of 8-aza-7-deazaguanine DNA bearing branched side chains with terminal triple bonds
  作者：Frank Seela、Hai Xiong、Simone Budow

  DOI：10.1016/j.tet.2010.03.086

  日期：2010.5

  2′-deoxyguanosine surrogates. Tm measurements demonstrate that compound 1 has a positive effect on duplex stability, which is comparable to the stabilizing effect of the octa-1,7-diynylated non-branched nucleoside 2. Nucleoside 1 and corresponding oligonucleotides were functionalized by the Cu(I)-mediated 1,3-dipolar cycloaddition ‘double click’ reaction with diverse ligands (AZT 3, benzyl azide 4

  由7-碘-8-氮杂-7-脱氮基合成8-氮杂-7-脱氮杂-2'-脱氧鸟苷（7）的7- [二（丙-2-炔基）氨基]丙-1-炔基衍生物（1）。Sonogashira将-2'-deoxyguanosine（7）交叉偶联并转化为亚磷酰胺结构单元10。通过固相合成制备带有带有末端三键的分支侧链的寡核苷酸，该固相合成包含单个或多个残基为1'的2'-脱氧鸟苷替代物。T m测量表明，化合物1对双链体稳定性有积极影响，这与octa-1,7-二酰化非支链核苷2的稳定作用相当。核苷1和相应的寡核苷酸，通过将Cu（I）介导的官能1,3-偶极环加成与不同的配体（AZT“双击”反应3，苄基叠氮4，11-azidoundecanol 5和M-dPEG™ 4 -叠氮化物6）。共轭反应在溶液和固体载体上进行。核苷1允许两个报告基团对单个残基进行“双重”官能化。即使有两个核苷酸1残基，“双击”反应仍可顺利进行被安排在近端位置。
• 8-Aza-7-deazaguanine nucleosides and oligonucleotides with octadiynyl side chains: synthesis, functionalization by the azide-alkyne ‘click’ reaction and nucleobase specific fluorescence quenching of coumarin dye conjugates
  作者：Frank Seela、Hai Xiong、Peter Leonard、Simone Budow

  DOI：10.1039/b822041g

  日期：——

  chains have steric freedom in duplex DNA. Nucleoside 2d as well as 2d-containing oligonucleotides were conjugated to the non-fluorescent 3-azido-7-hydroxycoumarin 15 by the Huisgen-Meldal-Sharpless ‘click’ reaction. Pyrazolo[3,4-d]pyrimidine nucleoside conjugate 16 shows a much higher fluorescence intensity than that of the corresponding pyrrolo[2,3-d]pyrimidine derivative 17. The quenching in the dye conjugate

  通过固相合成制备了 掺入8-氮杂-7-脱氮杂-2'-脱氧鸟苷（2d）的7-（八-1,7-二炔基）衍生物的寡核苷酸。通过Sonogashira交叉偶联反应引入2d的侧链，并合成亚磷酰胺（3a，3b）。与包含未官能化的8-氮杂7-脱氮基2'-脱氧鸟苷（2a）的双链体相比，含2d的双链体更加稳定，这表明这些侧链在双链体DNA中具有空间自由度。将核苷2d和含2d的寡核苷酸偶联到非荧光的3-azido-7-羟基香豆素上15，由Huisgen-Meldal-Sharpless做出的“点击”反应。吡唑并[3，4- d ]嘧啶核苷共轭物16显示出比相应的吡咯并[2，3- d ]嘧啶衍生物17更高的荧光强度。发现在染料缀合物17中的猝灭在单体缀合物阶段比在单链或双链DNA中更强。提出了核碱基-染料接触复合物，当核碱基是堆叠的一部分时，其在单体状态下比在DNA链中更有利。具有笨重的染料缀合物的侧链很好地容纳在
• TheHigh-Anti Conformation of 7-Halogenated 8-Aza-7-deaza-2′-deoxy-guanosines: A Study of the Influence of Modified Bases on the Sugar Structure of Nucleosides
  作者：Frank Seela、Georg Becher、Helmut Rosemeyer、Hans Reuter、Guido Kastner、Igor A. Mikhailopulo

  DOI：10.1002/(sici)1522-2675(19990113)82:1<105::aid-hlca105>3.0.co;2-3

  日期：1999.1.13

  The conformation of the 7-bromo- and 7-iodo-substituted 8-aza-7-deazapurine nucleosides 1 and 2 in the solid state and in aqueous solution was studied by single-crystal X-ray analyses and by H-1-NMR spectroscopy. In the solid state, both compounds display a high-anti conformation around the glycosylic bond, and their 2'-deoxy-beta-D-ribofuranose moieties adopt an N-type sugar puckering. The orientation of the exocyclic C(4')-C(5') bond was found to be crp in both cases. In D2O solution, both compounds display i) an 8-10% higher N-conformer population than 2'-deoxyguanosine and ii) a preference of the -sc conformation about the C(4')-C(5') bond. A comparative study on the influence of modified bases on the sugar structure of nucleosides is made.