精氨酸beta-萘甲酰胺 | 7182-70-9

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 中文名称: 精氨酸beta-萘甲酰胺
• 英文名称: L-arginine β-naphthylamide
• 英文别名: L-arginine-β-naphthylamide；Arg-βNA；Arg β-naphthylamide；L-Arg-β-NA；L-Arginin-；L-Arginin-β-naphthylamin；Arginine beta-naphthylamide；(2S)-2-amino-5-(diaminomethylideneamino)-N-naphthalen-2-ylpentanamide
• CAS: 7182-70-9
• 化学式: C₁₆H₂₁N₅O
• 分子量: 299.376
• InChiKey: DICSXQHGGOAUNU-AWEZNQCLSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  0.6
• 重原子数：
  22
• 可旋转键数：
  6
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.25
• 拓扑面积：
  120
• 氢给体数：
  4
• 氢受体数：
  3

反应信息

• 作为反应物：
  描述：

  精氨酸beta-萘甲酰胺 在 phosphate buffer 、 bovine leukocyte aminopeptidase (BL-APase) 作用下， 生成 L-精氨酸
  参考文献：
  名称：

  .BETA.-Naphthylamides of guanidinophenyl amino acids as substrates of aminopeptidases.

  摘要：

  合成了对-胍基-L-苯丙氨酸（GPA）和对-胍基-DL-苯甘氨酸（GPG）的β-萘甲酰胺，并将其作为牛白细胞氨肽酶（BL-APase）和猪肝氨肽酶 B（PL-APaseB）的底物与 L-精氨酸β-萘甲酰胺（Arg-βNA）进行了比较试验。BL-APase 催化 GPA-βNA 的水解速度与 Arg-βNA 一样快，而 GPG-βNA 的水解速度要慢得多。BL-AP 酶水解 GPA-βNA 的特异性常数（Vmax/Km）略大于水解 Arg-βNA。根据两种 β-萘甲酰胺底物 Km 值的比较，GPA-βNA 侧链中的苯环被认为有助于该底物与该酶特异性侧的结合。在底物浓度高于约 0.1 mM 的范围内，观察到 BL-APase 在水解 GPA-βNA 时存在底物抑制作用。PL-APaseB 不水解 GPA-βNA 也不水解 GPG-βNA，而且它们抑制该酶水解 Arg-βNA。预计 GPA-βNA 将成为研究氨基肽酶结合和催化特异性的有用底物。

  DOI：

  10.1248/cpb.36.1205
• 作为产物：
  描述：

  N^α-Benzyloxycarbonyl-L-arginin- 在 palladium on activated charcoal 盐酸 、 氢气 作用下， 以 甲醇 为溶剂， 生成 精氨酸beta-萘甲酰胺
  参考文献：
  名称：

  Goldstein,T.P. et al., Journal of medicinal and pharmaceutical chemistry, 1962, vol. 5, p. 852 - 857

  摘要：

  DOI：

文献信息

• Purification of an Aminopeptidase Preferentially Releasing N-terminal Alanine from Cucumber Leaves and Its Identification as a Plant Aminopeptidase N
  作者：Yasuo YAMAUCHI、Yukinori EJIRI、Kiyoshi TANAKA

  DOI：10.1271/bbb.65.2802

  日期：2001.1

  In this study, a highly active foliar aminopeptidase preferentially releasing N-terminal alanine from artificial substrates was purified and characterized from cucumber (Cucumis sativus L. suyo). The enzyme had a molecular mass of 200 kDa consisting of two subunits of 95 kDa. It was a metalloprotease the pH optimum of which was 8 to 9. It cleaved Ala-, Gly-, Met-, Ser-, Leu-, Lys-, and Arg artificial substrates. An internal amino acid sequence was similar to those of aminopeptidase N (clan MA, family M1) of microorganisms, and was very similar to that of a putative aminopeptidase N of Arabidopsis thaliana. From these results, the highly active aminopeptidase in cucumber leaves was identified to be a plant aminopepitdase N.

  在本研究中，从黄瓜（Cucumis sativus L. suyo）中纯化和鉴定了一种高活性的叶面氨基肽酶，该酶优先从人工底物中释放N端丙氨酸。该酶的分子质量为200 kDa，由两个95 kDa的亚基组成。它是一种金属蛋白酶，其最适pH为8至9。它能切割Ala-、Gly-、Met-、Ser-、Leu-、Lys-和Arg的人工底物。其内部氨基酸序列与微生物的氨基肽酶N（MA家族，M1家族）相似，并且与拟南芥中假定的氨基肽酶N非常相似。根据这些结果，黄瓜叶中高活性的氨基肽酶被鉴定为植物氨基肽酶N。
• DEVICE AND METHOD FOR SOLUBILIZING, SEPARATING, REMOVING AND REACTING CARBOXYLIC ACIDS IN OILS, FATS, AQUEOUS OR ORGANIC SOLUTIONS BY MEANS OF MICRO-OR NANOEMULSIFICATION
  申请人：Dietz Ulrich

  公开号：US20130090488A1

  公开（公告）日：2013-04-11

  The present invention is directed to solubilizing compounds, a device and a method for solubilizing and removing carboxylic acids and especially fatty acids from oils, fats, aqueous emulsion, aqueous media and organic solutions. Devices utilizing the inventive method shall be used for separating carboxylic acids from oils, fats, aqueous emulsion, lipophilic media or organic solutions, respectively by preparing an aqueous micro- or nanoemulsion of the carboxylic acids especially the fatty acids and the solubilizing compound which contains at least one amidino and/or gianidino group. Solubilization effects of solubilizing compounds combined with the inventive use of separation methods for carboxylic acids can be used to treat persons in need of removal of fatty acids or analyze carboxylic acids from blood or process other solutions in food, pharmacy, chemistry, bio fuel industry or other industrial processings.

  本发明涉及溶解化合物、用于从油、脂肪、水乳液、水介质和有机溶液中溶解和去除羧酸，特别是脂肪酸的装置和方法。采用本发明方法的装置将用于通过制备羧酸特别是脂肪酸和含有至少一种氨基甲酰基和/或肼基团的溶解化合物的水微观或纳米乳液，分离油、脂肪、水乳液、亲脂性介质或有机溶液中的羧酸。溶解化合物的溶解效果与本发明所述的羧酸分离方法相结合，可用于治疗需要去除脂肪酸或从血液中分析羧酸或处理食品、药品、化学、生物燃料工业或其他工业加工过程中的其他溶液的人员。
• A process for the identification of bacteria and a kit of reagents for use in this process
  申请人：NATIONAL RESEARCH DEVELOPMENT CORPORATION

  公开号：EP0018825A1

  公开（公告）日：1980-11-12

  A procedure for identification of bacteria comprises subjecting the bacteria to a combination of tests for determination of 26 bacterial enzymes, permitting rapid identification by use of tests generally adapted for determination of constitutive enzymes. The procedure generally permits more rapid identification than previous bacterial identification procedures and is universally applicable to a very wide range of bacteria including most bacteria which are commonly encountered clinically. In a particular embodiment the invention includes a procedure for rapid differentation of the bacterial groups Escherichia, Klebsiella spp., Proteus and Pseudomonas spp., comprising subjecting the bacteria to a combination of tests for determination of bacterial acid phosphatase. beta-galactosidase, glutamate decarboxylase, phenylalanine deaminase, cytochrome oxidase, diacetyl producing enzymes and urease. Additionally the invention includes kits for carrying out the procedures of the invention. typically comprising separate specific substrates for each of the enzymes which it is desired to determine, the kit being preferably in the form of a test card or other suitable apparatus comprising a plurality of wells or compartments which separately contain the enzyme substrates.

  细菌鉴定程序包括对细菌进行测定 26 种细菌酶的组合试验，通过使用一般适用于测定组成酶的试验，可快速鉴定细菌。与以前的细菌鉴定程序相比，该程序一般能更快地鉴定细菌，而且普遍适用于各种细菌，包括临床上常见的大多数细菌。在一个特定的实施方案中，本发明包括一种用于快速鉴别埃希氏菌、克雷伯氏菌属、变形杆菌和假单胞菌属细菌的程序，包括对细菌进行测定细菌酸性磷酸酶、β-半乳糖苷酶、谷氨酸脱羧酶、苯丙氨酸脱氨酶、细胞色素氧化酶、双乙酰产酶和脲酶的组合试验。此外，本发明还包括用于执行本发明程序的试剂盒。试剂盒通常包括用于需要测定的每种酶的单独特异性底物，试剂盒最好采用测试卡或其他适当装置的形式，该装置包括多个分别含有酶底物的孔或隔室。
• Biologically active composition and immunoassay using the same
  申请人：FUJIREBIO KABUSHIKI KAISHA also trading as FUJIREBIO INC.

  公开号：EP0094777A1

  公开（公告）日：1983-11-23

  A biologically active composition comprises an immobilised antigen or antibody, phase, and an immobilised enzyme enzyme inhibitor or enzyme activator phase. The composition may be used in determination of antigen or antibody in a simple procedure of high sensitivity.

  生物活性组合物由固定化抗原或抗体相和固定化酶抑制剂或酶活化剂相组成。该组合物可用于以高灵敏度的简单程序测定抗原或抗体。
• The novel microorganism strain Chromobacterium violaceum, pharmaceutically active compounds being produced by the strain, a process for preparing the compounds and the use of these compounds as medicaments
  申请人：MICROBIAL CHEMISTRY RESEARCH FOUNDATION

  公开号：EP0096356A2

  公开（公告）日：1983-12-21

  The present invention relates to the novel microorganism strain Chromobacterium violaceum, comDounds of the formula wherein R is hydrogen or the hydroxyl group, being produced by the afore-mentioned strain and their use as medicaments.

  本发明涉及新型微生物菌株 Chromobacterium violaceum、由上述菌株产生的式中 R 为氢或羟基的化合物及其作为药物的用途。 其中 R 为氢或羟基，由上述菌株产生并用作药物。