The present invention relates to the detection of B. gingivalis by the specific ability of B. gingivalis to hydrolyze N-carbobenzoxy-glycyl-glycyl-L-arginine-B-napthylamide derivatives. The present invention also relates to the use of assay systems which inhibit serum amino peptidase and enhance the detection of the B. gingivalis N-CBz-Gly-Gly-Arg peptidase. The assay system inhibits the serum enzyme by the use of a serum aminopeptidase inhibitor which inhibits the activity of serum aminopeptidase to a greater extent than it inhibits the activity of B. gingivalis N-CBz-Gly-Gly-Arg peptidase. The assay system also increases the reliability of detection of the B. gingivalis enzyme by the use of enhancing materials which enhance the enzyme activity of B. gingivalis N-CBz-Gly-Gly-Arg peptidase to a greater extent than such materials enhance the enzyme activity of serum aminopeptidase.
本发明涉及通过牙龈杆菌
水解 N-羧基
苯氧基-甘
氨酰-甘
氨酰-L-精
氨酰-B-
萘酰胺衍
生物的特异性能力来检测牙龈杆菌。本发明还涉及抑制血清
氨基肽酶并提高牙龈杆菌 N-CBz-Gly-Gly-Arg 肽酶检测能力的检测系统的使用。该检测系统通过使用血清
氨基肽酶抑制剂来抑制血清酶,该
抑制剂对血清
氨基肽酶活性的抑制程度大于对牙龈杆菌 N-CBz-Gly-Gly-Arg 肽酶活性的抑制程度。该检测系统还通过使用增强材料提高牙龈杆菌 N-CBz-Gly-Gly-Arg 肽酶的酶活性,其增强程度大于此类材料对血清
氨肽酶酶活性的增强程度,从而提高检测牙龈杆菌酶的可靠性。