4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid potassium salt | 115144-35-9

• 物质功能分类: 化学试剂 - 有机试剂
• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 英文名称: 4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid potassium salt
• 英文别名: potassium;2-(6-hydroxy-1,3-benzothiazol-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylate
• CAS: 115144-35-9
• 化学式: C11H7KN2O3S2
• 分子量: 318.4
• InChiKey: UMBKGTQQGYPQBE-UHFFFAOYSA-M

物化性质

• 溶解度：
  DMF:16.7(Max Conc. mg/mL);52.45(Max Conc. mM)DMSO:7.5(Max Conc. mg/mL);23.55(Max Conc. mM)Ethanol:0.25(Max Conc. mg/mL);0.79(Max Conc. mM)PBS (pH 7.2):10.0(Max Conc. mg/mL);0.31(Max Conc. mM)Water:32.0(Max Conc. mg/mL);100.5(Max Conc. mM)

计算性质

• 辛醇/水分配系数(LogP)：
  -1.68
• 重原子数：
  19
• 可旋转键数：
  2
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.18
• 拓扑面积：
  139
• 氢给体数：
  1
• 氢受体数：
  7

安全信息

• 海关编码：
  2934999090
• 危险性防范说明：
  P261,P305+P351+P338
• 危险性描述：
  H302,H315,H319,H335

制备方法与用途

简介

D-荧光素钾盐是荧光素酶的水溶性底物，存在于多种发光生物体中。在ATP和荧光素酶的催化作用下，D-荧光素钾被氧化，产生蓝绿色的光(560nm)，当底物过量时，产生的光量子数与荧光素酶的浓度呈正相关。编码荧光素酶的Luc基因是植物、哺乳动物细胞的常用报告基因。由于没有背景干扰，因此可以很容易地检测出低至0.02 pg 水平的荧光素酶。

用途

D-荧光素钾盐是一类在生物体中发现的能引起生物发光的杂环化合物，如萤火虫。在ATP存在下，萤光素酶将其氧化脱羧后会发光。化学研究中可用于荧光素酶的基板。

生物活性

D-Luciferin是萤火虫荧光素酶的底物。

体外研究

D-luciferin is the natural substrate of the enzyme luciferase (Luc), that catalyzes the production of the typical yellowgreen light of fireflies.The present review covers the synthesis of D-luciferin and derivatives or analogues that are substrates or inhibitors of the luciferase from the American firefly Photinus pyralis , the enzyme more frequently used in techniques of in vitro and optical imaging.
D-Luciferin exhibits a decrease in the measured K _m in PC3M-Luc cell lysates with a K _m of 34 μM.

体内研究

Bioluminescence imaging (BLI) using the firefly luciferase (Fluc) as a reporter gene and D-luciferin as a substrate is currently the most widely employed technique. The total signal intensity is plotted against the time after D-luciferin injection to generate a time-intensity curve. In addition to the peak signal, the signals at fixed time points (5, 10, 15, and 20 min) after D-luciferin injection are determined as alternatives to the peak signal. The signal in a given time-intensity curve is normalized for the peak signal in the curve to represent the pattern of temporal changes after D-luciferin injection.
Inject with 10 μL of D-luciferin (intraperitoneally or intravenously) stock solution per gram of body weight: normally ~200 μL for a 20 g mouse for a standard 150 mg/kg injection.
Thaw D-Luciferin (either Potassium or Sodium Salt) at room temperature and dissolve in dPBS (no calcium or magnesium) to a final concentration of 15 mg/mL. Pre-wet a 0.22 μm filter by drawing through 5-10 mL of sterile H ₂ O and discard water. Sterilize the D-Luciferin solution through the prepared 0.22 μm syringe filter.

用途

活细胞、组织或生物体内luc标记基因和荧光素酶-融合基因体内/体外表达的成像分析； 2）广泛用于报告基因分析，免疫分析和ATP荧光卫生监测分析；

文献信息

• Humanized anti-human-PD-1 antibody and its use in cancer treatment
  申请人：OSE IMMUNOTHERAPEUTICS

  公开号：US11352430B2

  公开（公告）日：2022-06-07

  Described herein are humanized anti-PD-1 antibodies, nucleic acids encoding such, and uses thereof in enhancing immune responses by activating T cells and treating diseases such as cancer and an infectious disease.

  本描述中提供了人源化抗PD-1抗体、编码此类的核酸及其在通过激活T细胞增强免疫反应以及治疗癌症和传染病等疾病中的应用。
• HUMANIZED ANTI-HUMAN-PD-1 ANTIBODY
  申请人：OSE Immunotherapeutics

  公开号：EP3883966A1

  公开（公告）日：2021-09-29
• [EN] HUMANIZED ANTI-HUMAN-PD-1 ANTIBODY<br/>[FR] ANTICORPS ANTI-PD-1 ANTI-HUMAIN HUMANISÉ
  申请人：OSE IMMUNOTHERAPEUTICS

  公开号：WO2020127366A1

  公开（公告）日：2020-06-25

  Described herein are humanized anti-PD-1 antibodies, nucleic acids encoding such, and uses thereof in enhancing immune responses by activating T cells and treating diseases such as cancer and an infectious disease.
• [EN] BIFUNCTIONAL ANTI-PD1/IL-7 MOLECULES<br/>[FR] MOLÉCULES BIFONCTIONNELLES ANTI-PD1/IL-7
  申请人：[en]OSE IMMUNOTHERAPEUTICS

  公开号：WO2022129512A1

  公开（公告）日：2022-06-23

  The present invention relates to bifunctional molecules comprising an IL-7 variant and having a particular scaffold and their uses.