3-carboxy-3-hydroxy-5-methylhexanoic acid | 60924-65-4

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羟基酸及其衍生物
• 英文名称: 3-carboxy-3-hydroxy-5-methylhexanoic acid
• 英文别名: 2-Hydroxy-2-(2-methylpropyl)-bernsteinsaeure；3-Hydroxy-3-carboxy-5-methyl-hexansaeure；Isobutylmalic acid；2-hydroxy-2-(2-methylpropyl)butanedioic acid
• CAS: 60924-65-4
• 化学式: C₈H₁₄O₅
• 分子量: 190.196
• InChiKey: AOJRRQNPQBDATA-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  0.2
• 重原子数：
  13
• 可旋转键数：
  5
• 环数：
  0.0
• sp3杂化的碳原子比例：
  0.75
• 拓扑面积：
  94.8
• 氢给体数：
  3
• 氢受体数：
  5

反应信息

• 作为反应物：
  描述：

  3-carboxy-3-hydroxy-5-methylhexanoic acid 在 lithium aluminium tetrahydride 、 二苯并-18-冠醚-6 、 potassium carbonate 作用下， 以 四氢呋喃 、 乙腈 为溶剂， 反应 20.0h， 生成 3-hydroxy-3-hydroxymethyl-5-methyl-1-hexanol
  参考文献：
  名称：

  Biosynthesis of the Cephalotaxus alkaloids. Investigations of the biosynthesis of deoxyharringtonine, isoharringtonine, and harringtonine

  摘要：

  DOI：

  10.1021/ja00526a051

文献信息

• Probe compound for detecting and isolating enzymes and means and methods using the same
  申请人：Helmholtz-Zentrum für Infektionsforschung GmbH

  公开号：EP2230312A1

  公开（公告）日：2010-09-22

  The present invention relates to a probe compound that can comprise any substrate or metabolite of an enzymatic reaction in addition to an indicator component, such as, for example, a fluorescence dye, or the like. Moreover, the present invention relates to means for detecting enzymes in form of an array, which comprises any number of probe compounds of the invention which each comprise a different metabolite of interconnected metabolites representing the central pathways in all forms of life. Moreover, the present invention relates to a method for detecting enzymes involving the application of cell extracts or the like to the array of the invention which leads to reproducible enzymatic reactions with the substrates. These specific enzymatic reactions trigger the indicator (e.g. a fluorescence signal) and bind the enzymes to the respective cognate substrates. Moreover, the invention relates to means for isolating enzymes in form of nanoparticles coated with the probe compound of the invention. The immobilisation of the cognate substrates or metabolites on the surface of nanoparticles by means of the probe compounds allows capturing and isolating the respective enzyme, e.g. for subsequent sequencing.

  本发明涉及一种探针化合物，它可以包括酶反应的任何底物或代谢物，此外还包括指示成分，例如荧光染料或类似物。此外，本发明还涉及以阵列形式检测酶的方法，该阵列由任意数量的本发明探针化合物组成，每种探针化合物由代表所有生命形式中中心途径的相互关联的代谢物中的不同代谢物组成。此外，本发明还涉及一种检测酶的方法，该方法涉及将细胞提取物或类似物应用于本发明的阵列，从而导致与底物发生可重复的酶反应。这些特定的酶反应会触发指示剂（如荧光信号），并将酶与各自的同源底物结合。此外，本发明还涉及以涂覆有本发明探针化合物的纳米颗粒形式分离酶的方法。通过探针化合物将同源底物或代谢物固定在纳米颗粒表面，可以捕获和分离相应的酶，例如用于后续测序。
• Isopropylmalate synthase from Nicotiana tabacum and methods and uses thereof
  申请人：Philip Morris Products S.A.

  公开号：EP2565265A1

  公开（公告）日：2013-03-06

  The present invention relates to a mutant, non-naturally occurring or transgenic plant cell comprising: (i) at least one polynucleotide comprising, consisting or consisting essentially of a sequence encoding an isopropylmalate synthase and having at least 60% sequence identity to SEQ ID NO:1 or SEQ ID NO:10 or SEQ ID NO: 12 or SEQ ID NO:14; or (ii) a polypeptide encoded by said polynucleotide(s); or (iii) a polypeptide having at least 60% sequence identity to SEQ ID NO:2 or SEQ ID NO:11 or SEQ ID NO:13 or SEQ ID NO:15; or (iv) a construct, vector or expression vector comprising said polynucleotide sequence(s), optionally wherein said construct, vector or expression vector additionally comprises a promoter comprising, consisting or consisting essentially of the sequence set forth in SEQ ID NO:8 or a variant thereof with at least about 60% identity thereto or a trichome promoter.

  本发明涉及一种突变体、非天然产生的或转基因植物细胞，该细胞包含：(i) 至少一种多核苷酸，该多核苷酸由编码异丙基丙二酸合成酶的序列组成，或主要由编码异丙基丙二酸合成酶的序列组成，并且与SEQ ID NO：1 或 SEQ ID NO:10 或 SEQ ID NO:12 或 SEQ ID NO:14；或 (ii) 由所述多核苷酸编码的多肽；或 (iii) 与 SEQ ID NO：2 或 SEQ ID NO:11 或 SEQ ID NO:13 或 SEQ ID NO:15；或(iv)包含所述多核苷酸序列的构建体、载体或表达载体，可选地，其中所述构建体、载体或表达载体另外包含一个启动子，该启动子由 SEQ ID NO:8 所述序列或其至少约 60% 相同的变体或毛状体启动子组成或基本由其组成。
• THE USE OF SUCCINATE DERIVATIVE ESTERS FOR THE TREATMENT OF DEMENTIA
  申请人：THE INSTITUTE OF MATERIA MEDICA OF CHINESE ACADEMY OF MEDICAL SCIENCES

  公开号：EP1582209B1

  公开（公告）日：2012-09-19
• ISOPROPYLMALATE SYNTHASE FROM NICOTIANA TABACUM AND METHODS AND USES THEREOF
  申请人：Bakaher Nicholas

  公开号：US20140352706A1

  公开（公告）日：2014-12-04

  The present invention relates to a mutant, non-naturally occurring or transgenic plant cell comprising: (i) at least one polynucleotide comprising, consisting or consisting essentially of a sequence encoding an isopropylmalate synthase and having at least 60% sequence identity to SEQ ID NO:1 or SEQ ID NO:10 or SEQ ID NO: 12 or SEQ ID NO:14; or (ii) a polypeptide encoded by said polynucleotide(s); or (iii) a polypeptide having at least 60% sequence identity to SEQ ID NO:2 or SEQ ID NO:11 or SEQ ID NO:13 or SEQ ID NO:15; or (iv) a construct, vector or expression vector comprising said polynucleotide sequence(s), optionally wherein said construct, vector or expression vector additionally comprises a promoter comprising, consisting or consisting essentially of the sequence set forth in SEQ ID NO:8 or a variant thereof with at least about 60% identity thereto or a trichome promoter.
• US7169774B2
  申请人：——

  公开号：US7169774B2

  公开（公告）日：2007-01-30