Val-Leu | 3929-62-2

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 英文名称: Val-Leu
• 英文别名: N-valyl-leucine；Valyl=>leucin；N-Valyl-leucin；inakt.Valylleucin；valylleucine；2-[(2-azaniumyl-3-methylbutanoyl)amino]-4-methylpentanoate
• CAS: 3929-62-2
• 化学式: C₁₁H₂₂N₂O₃
• 分子量: 230.307
• InChiKey: XCTHZFGSVQBHBW-UHFFFAOYSA-N

物化性质

• 熔点：
  247-248 °C
• 沸点：
  421.9±30.0 °C(Predicted)
• 密度：
  1.066±0.06 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  -2.1
• 重原子数：
  16
• 可旋转键数：
  6
• 环数：
  0.0
• sp3杂化的碳原子比例：
  0.82
• 拓扑面积：
  92.4
• 氢给体数：
  3
• 氢受体数：
  4

反应信息

• 作为反应物：
  描述：

  Val-Leu 、 水 生成 DL-亮氨酸
  参考文献：
  名称：

  Abderhalden; Komm, Hoppe-Seyler's Zeitschrift fur Physiologische Chemie, 1924, vol. 139, p. 156

  摘要：

  DOI：
• 作为产物：
  描述：

  N-苄氧羰基-DL-缬氨酸 在 1,4-二氧六环 、 甲醇 、 三正丁胺 、 氯甲酸乙酯 、 钯 作用下， 生成 Val-Leu
  参考文献：
  名称：

  Davis, Annales de Chimie (Cachan, France), 1954, vol. <12> 9, p. 399,426

  摘要：

  DOI：

文献信息

• SUBSTRATE FOR ASSAYING BETA-GLUCAN AND/OR ENDOTOXIN AND ASSAY METHOD
  申请人：Kitagawa Takeshi

  公开号：US20100330700A1

  公开（公告）日：2010-12-30

  An object of the present invention is to provide a peptide derivative for determining β-glucan or endotoxin which allows high sensitivity measurement, and a method for determining β-glucan and/or endotoxin using the same. The present invention relates to (1) a peptide derivative represented by the following general formula [1]: X-A 1 -Gly-Arg-A 2 -E-D  [1], (2) a reagent for determining β-glucan and/or endotoxin comprising the above-described peptide derivative, (3) a method for determining β-glucan and/or endotoxin, characterized in that a sample containing β-glucan and/or endotoxin, an amebocyte lysate of a horseshoe crab and the above-described peptide derivative are reacted each other, then the resulting released compound represented by the following general formula [2]: H-A 2 -E-D  [2] is separated from unreacted substance and quantified, and the determination is made based on this value, and (4) a reagent kit for determining β-glucan and/or endotoxin, comprising an amebocyte lysate of a horseshoe crab and the above-described peptide derivative as constituents thereof.

  本发明的目的是提供一种用于测定β-葡聚糖或内毒素的肽衍生物，该肽衍生物允许高灵敏度测量，并提供使用该肽衍生物测定β-葡聚糖和/或内毒素的方法。本发明涉及(1)由以下通式[1]表示的肽衍生物：X-A1-Gly-Arg-A2-E-D[1]，(2)用上述肽衍生物的试剂用于测定β-葡聚糖和/或内毒素，(3)一种用于测定β-葡聚糖和/或内毒素的方法，其特征在于将含有β-葡聚糖和/或内毒素的样品、一种马蹄蟹的阿米巴溶解液和上述肽衍生物反应，然后分离出代表以下通式[2]的所释放的化合物：H-A2-E-D[2]，并对此值进行定量测定，然后进行测定，以及(4)用于测定β-葡聚糖和/或内毒素的试剂盒，其包括一种马蹄蟹的阿米巴溶解液和上述肽衍生物作为其组分。
• REAGENT CONTAINING PROTEASE REACTION PROMOTER AND/OR COLORANT STABILIZER
  申请人：Asahi Kasei Pharma Corporation

  公开号：EP1788081A1

  公开（公告）日：2007-05-23

  [PROBLEMS] To provide a protease reaction promoter being superior in usefulness to the known protease reaction promoters, a stabilizer for a colorant to be used in detecting an enzymatic reaction, a method therefor and a reagent and a method for measuring a glycosylated protein and the ratio of a glycosylated protein with the use of the same. [MEANS FOR SOLVING PROBLEMS] A protease reaction promoter which contains at least one member selected from among an acetate group-containing compound or its salt, N-acyltaurine or its salt and a polyoxyethylene alkyl ether sulfate or its salt; a method of promoting a protease reaction by using this protease reaction promoter, a stabilizer reagent containing cyclodextrin for stabilizing a colorant to be used in detecting an enzymatic reaction; a method of stabilization via the coexistence of cyclodextrin with the above colorant; and a reagent and a method for measuring a glycosylated protein and the ratio of a glycosylated protein with the use of the protease reaction promoter and/or the reagent containing cyclodextrin as described above.

  [问题] 提供一种在实用性上优于已知蛋白酶反应促进剂的蛋白酶反应促进剂、一种用于检测酶反应的着色剂的稳定剂、一种用于此的方法以及一种使用该促进剂和稳定剂测量糖基化蛋白质和糖基化蛋白质比率的试剂和方法。[解决问题的方法]一种蛋白酶反应促进剂，它含有至少一种选自含醋酸基的化合物或其盐、N-酰基牛磺酸或其盐和聚氧乙烯烷基醚硫酸盐或其盐中的成员；一种通过使用这种蛋白酶反应促进剂促进蛋白酶反应的方法，一种含有环糊精的稳定剂试剂，用于稳定检测酶反应中使用的着色剂；通过环糊精与上述着色剂共存进行稳定的方法；以及使用上述蛋白酶反应促进剂和/或含有环糊精的试剂测量糖基化蛋白质和糖基化蛋白质比率的试剂和方法。
• NOVEL DIPEPTIDE-DIGESTING ENZYME, METHOD OF PRODUCING THE SAME, METHOD OF ASSAYING GLYCATED PROTEIN BY USING THE DIPEPTIDE-DIGESTING ENZYME AND REAGENT COMPOSITION TO BE USED THEREIN
  申请人：Amano Enzyme Inc.

  公开号：EP2093288A1

  公开（公告）日：2009-08-26

  Provided are a dipeptide-degrading enzyme which acts on a dipeptide to produce amino acids; a novel measurement method for quickly, simply, and accurately measuring a glycosylated protein, a glycosylated peptide, or a glycosylated dipeptide, including allowing the above enzyme to act on a glycosylated peptide to produce a glycosylated amino acid and an amino acid, and quantifying the amino acids; a dipeptide-degrading enzyme which acts on a dipeptide to produce amino acids, the dipeptide being a glycosylated dipeptide, and the amino acids including a glycosylated amino acid and an amino acid; a method for producing a glycosylated amino acid and an amino acid, including allowing a protease and a dipeptide-degrading enzyme to act on a specimen containing a glycosylated protein or a glycosylated peptide; a method for measuring a glycosylated protein, a glycosylated peptide, or a glycosylated dipeptide, including allowing the amino acid produced by the above method to be degraded by a substance which degrades the amino acid, and quantifying the amino acid.

  本发明提供了一种二肽降解酶，该酶作用于二肽以产生氨基酸；一种新颖的测量方法，用于快速、简单、准确地测量糖基化蛋白质、糖基化肽或糖基化二肽，包括让上述酶作用于糖基化肽以产生糖基化氨基酸和氨基酸，并对氨基酸进行定量；一种二肽降解酶，其作用于二肽以产生氨基酸，该二肽为糖基化二肽，氨基酸包括糖基化氨基酸和氨基酸；一种生产糖基化氨基酸和一种氨基酸的方法，包括让蛋白酶和二肽降解酶作用于含有糖基化蛋白质或糖基化肽的试样；一种测定糖基化蛋白质、糖基化肽或糖基化二肽的方法，包括让上述方法生产的氨基酸被降解氨基酸的物质降解，并对氨基酸进行定量。
• NOVEL FRUCTOSYL PEPTIDE OXIDASE
  申请人：Kyowa Medex CO., LTD.

  公开号：EP2357228A1

  公开（公告）日：2011-08-17

  A protein described in any one of [1] to [4] below is provided according to the present invention: [1] a protein comprising the amino acid sequence represented by SEQ ID NO: 1; [2] a protein comprising an amino acid sequence with one or more amino acid deletions, substitutions, or additions in the amino acid sequence represented by SEQ ID NO: 1, and having fructosyl peptide oxidase activity; [3] a protein comprising an amino acid sequence having 80% or higher homology to the amino acid sequence represented by SEQ ID NO: 1, and having fructosyl peptide oxidase activity; and [4] a protein having fructosyl peptide oxidase activity, which is produced by an expression plasmid harbored by the Escherichia coli XL1-Blue MRF' strain deposited under Accession No. FERM BP-11026.

  根据本发明提供了以下[1]至[4]中任一项所述的蛋白质： [1]包含由 SEQ ID NO: 1 所代表的氨基酸序列的蛋白质； [2]一种蛋白质，其氨基酸序列在 SEQ ID NO: 1 所代表的氨基酸序列中具有一个或多个氨基酸缺失、取代或添加，并且具有果糖基肽氧化酶活性； [3] 一种蛋白质，其氨基酸序列与 SEQ ID NO: 1 所代表的氨基酸序列具有 80% 或更高的同源性，且具有果糖基肽氧化酶活性；以及 [4] 具有果糖基肽氧化酶活性的蛋白质，该蛋白质由大肠杆菌 XL1-Blue MRF' 菌株所携带的表达质粒产生，该菌株的登录号为 FERM BP-11026。
• Methods and systems for determining autism spectrum disorder risk
  申请人：Laboratory Corporation of America Holdings

  公开号：US10041932B2

  公开（公告）日：2018-08-07

  In certain embodiments, the invention stems from the discovery that analysis of population distribution curves of metabolite levels in blood can be used to facilitate predicting risk of autism spectrum disorder (ASD) and/or to differentiate between ASD and non-ASD developmental delay (DD) in a subject. In certain aspects, information from assessment of the presence, absence, and/or direction (upper or lower) of a tail effect in a metabolite distribution curve is utilized to predict risk of ASD and/or to differentiate between ASD and DD.

  在某些实施方案中，本发明源于发现血液中代谢物水平的群体分布曲线分析可用于促进预测自闭症谱系障碍（ASD）的风险和/或区分受试者的自闭症谱系障碍和非自闭症谱系障碍发育迟缓（DD）。在某些方面，评估代谢物分布曲线中尾部效应的存在、不存在和/或方向（上部或下部）的信息可用于预测 ASD 的风险和/或区分 ASD 和 DD。