γ-glutamyl-2-aminobutyrate | 1374040-50-2

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 英文名称: γ-glutamyl-2-aminobutyrate
• 英文别名: N-(γ-L-glutamyl)-α-aminobutyric acid；(2S)-2-amino-5-(1-carboxypropylamino)-5-oxopentanoic acid
• CAS: 1374040-50-2
• 化学式: C₉H₁₆N₂O₅
• 分子量: 232.236
• InChiKey: FUZOZPRKGAXGOB-ZBHICJROSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -3.2
• 重原子数：
  16
• 可旋转键数：
  7
• 环数：
  0.0
• sp3杂化的碳原子比例：
  0.67
• 拓扑面积：
  130
• 氢给体数：
  4
• 氢受体数：
  6

反应信息

• 作为产物：
  描述：

  monosodium L-glutamate 、 DL-2-氨基丁酸 在 recombinant (His)6-tagged Escherichia coli K12 W3110 γ-glutamylcysteine synthetase 、 magnesium sulfate 、 5’-三磷酸腺苷 作用下， 以 水 为溶剂， 反应 1.0h， 生成 γ-glutamyl-2-aminobutyrate
  参考文献：
  名称：

  A Novel Catalytic Ability of γ-Glutamylcysteine Synthetase ofEscherichia coliand Its Application in Theanine Production

  摘要：

  γ-谷氨酰半胱氨酸合成酶（γGCS，EC 6.3.2.2）以 ATP 依赖性方式催化 l-谷氨酸（Glu）和 l-半胱氨酸（Cys）形成γ-谷氨酰半胱氨酸。虽然γGCS可以使用多种氨基酸作为底物，但人们对它是否可以使用非氨基酸化合物代替Cys知之甚少。我们确定大肠杆菌中的γGCS能够将Glu和胺结合形成γ-谷氨酰胺。反应速率取决于胺的亚甲基链长度，顺序如下：正丙胺 > 丁胺 > 乙胺 >> 甲胺。与与氨基酸反应相比，该反应的最佳 pH 值更窄、碱性更强。新发现的 γGCS 催化能力被用于生产茶氨酸（γ-谷氨酰乙胺）。表达 γGCS 的大肠杆菌静息细胞通过糖酵解再生 ATP，在 18 小时内从 429 毫米乙胺中合成了 12.1 毫米茶氨酸。

  DOI：

  10.1271/bbb.90538

文献信息

• USE OF PEPTIDES FOR IMPARTING KOKUMI
  申请人：FUTAKI FUMIE

  公开号：US20120034364A1

  公开（公告）日：2012-02-09

  The inventors of this invention have conducted the search for a variety of compounds which may possess a desired CaSR agonist activity to thus find out a substance capable of imparting a kokumi, which shows a more excellent kokumi-imparting effect, in particular, a kokumi-imparting effect of the initial taste-imparting type one, which is excellent in the stability and which can easily be produced at a low cost and the present invention thus provide a kokumi-imparting agent consisting of such a substance as well as a complex kokumi-imparting agent comprising the substance and other substances possessing the CaSR agonist activities in combination. More particularly, the present invention herein provides a kokumi-imparting agent consisting of γ-Glu-Abu (L-γ-glutamyl-L-2-amino-butyric acid) and a complex kokumi-imparting agent comprising the foregoing substance and another substance having a CaSR agonist activity, in combination.

  本发明的发明者们已经寻找了多种可能具有所需CaSR激动剂活性的化合物，以此找到一种能够赋予kokumi的物质，该物质具有更出色的kokumi赋予效果，特别是初始味道赋予型的kokumi赋予效果，稳定性优异，成本低廉且易于生产。因此，本发明提供了由这种物质组成的kokumi赋予剂，以及由该物质和其他具有CaSR激动剂活性的物质组合而成的复合kokumi赋予剂。更具体地说，本发明提供了由γ-Glu-Abu（L-γ-谷氨酰-L-2-氨基丁酸）组成的kokumi赋予剂，以及由上述物质和另一种具有CaSR激动剂活性的物质组合而成的复合kokumi赋予剂。
• INDUCED MALIGNANT STEM CELLS
  申请人：National Cancer Center

  公开号：US20140137274A1

  公开（公告）日：2014-05-15

  PROBLEM There are provided induced malignant stem cells capable of in vitro proliferation that are useful in cancer research and drug discovery for cancer therapy, as well as processes for production thereof, cancer cells derived from these cells, and applications of these cells. MEANS FOR SOLVING An induced malignant stem cell capable of in vitro proliferation are characterized by satisfying the following two requirements: (1) having at least one aberration selected from among (a) an aberration of methylation (high or low degree of methylation) in a tumor suppressor gene or a cancer-related genetic region in endogenous genomic DNA, (b) a somatic mutation of a tumor suppressor gene or a somatic mutation of an endogenous cancer-related gene in endogenous genomic DNA, (c) abnormal expression (increased or reduced/lost expression) of an endogenous oncogene or an endogenous tumor suppressor gene, (d) abnormal expression (increased or reduced/lost expression) of a noncoding RNA such as an endogenous cancer-related microRNA, (e) abnormal expression of an endogenous cancer-related protein, (f) an aberration of endogenous cancer-related metabolism (hypermetabolism or hypometabolism), (g) an aberration of endogenous cancer-related sugar chain, (h) an aberration of copy number variations in endogenous genomic DNA, and (i) instability of microsatellites in endogenous genomic DNA in an induced malignant stem cell; and (2) expressing genes including POU5F1 gene, NANOG gene, SOX2 gene, and ZFP42 gene.

  问题：提供了一种在体外增殖的诱导恶性干细胞，可用于癌症研究和癌症治疗药物发现，以及其生产过程，从这些细胞衍生的癌细胞和这些细胞的应用。 解决方法：一种在体外增殖的诱导恶性干细胞，其特征在于满足以下两个要求： （1）具有来自内源基因组DNA中肿瘤抑制基因或癌症相关遗传区的甲基化异常（高度或低度甲基化），内源基因组DNA中肿瘤抑制基因的体细胞突变或内源性癌症相关基因的体细胞突变，内源性癌基因或内源性肿瘤抑制基因的异常表达（增加或减少/丢失表达），内源性癌症相关microRNA等非编码RNA的异常表达，内源性癌症相关蛋白质的异常表达，内源性癌症相关代谢异常（高代谢或低代谢），内源性癌症相关糖链的异常，内源基因组DNA中拷贝数变异的异常，以及诱导恶性干细胞中微卫星的不稳定性；以及 （2）表达包括POU5F1基因、NANOG基因、SOX2基因和ZFP42基因在内的基因。
• METHOD FOR DETERMINATION OF OXIDATIVE STRESS
  申请人：Keio University

  公开号：EP1975621A1

  公开（公告）日：2008-10-01

  Provided is a biomarker that enables easy and rapid detection of oxidative stress on a living organism and enables prevention of tissue damage or cell necrosis by drug administration, and which is a powerful marker for the study of toxicity and pharmacokinetics of various agents. Oxidative stress is determined by measuring blood concentration of ophthalmic acid, which is a substance that varies in blood depending on the variation of reduced glutathione (GSH) concentration in a biological sample with the use of an analyzer such as a capillary electrophoresis-mass spectrometer. Further, an anti-oxidative stress agent is screened by administering an anti-oxidative stress candidate agent to anon-human animal under oxidative stress conditions, measuring blood concentration of ophthalmic acid, and evaluating the degree of decrease in the ophthalmic acid concentration.

  本发明提供了一种生物标记物，它能方便快捷地检测生物体的氧化应激，并能防止给药造成组织损伤或细胞坏死，是研究各种药物毒性和药代动力学的有力标记物。氧化应激是通过测定血液中的眼药酸浓度来确定的，眼药酸是一种根据生物样本中还原型谷胱甘肽（GSH）浓度的变化而变化的物质，可使用毛细管电泳-质谱仪等分析仪进行测定。此外，通过在氧化应激条件下给非人类动物注射抗氧化应激候选药剂，测量血液中的眼药酸浓度，并评估眼药酸浓度的下降程度，从而筛选抗氧化应激药剂。
• USE OF PEPTIDE FOR IMPARTING BODY TASTE
  申请人：Ajinomoto Co., Inc.

  公开号：EP2415359A1

  公开（公告）日：2012-02-08

  The inventors of this invention have conducted the search for a variety of compounds which may possess a desired CaSR agonist activity to thus find out a substance capable of imparting a kokumi, which shows a more excellent kokumi-imparting effect, in particular, a kokumi-imparting effect of the initial taste-imparting type one, which is excellent in the stability and which can easily be produced at a low cost and the present invention thus provide a kokumi-imparting agent consisting of such a substance as well as a complex kokumi-imparting agent comprising the substance and other substances possessing the CaSR agonist activities in combination. More particularly, the present invention herein provides a kokumi-imparting agent consisting of γ -Glu-Abu (L- γ -glutamyl-L-2-amino- butyric acid) and a complex kokumi-imparting agent comprising the foregoing substance and another substance having a CaSR agonist activity, in combination.

  本发明的发明者对可能具有所需的 CaSR 激动剂活性的各种化合物进行了研究，从而找到了一种能够诱导果胶的物质，该物质显示出更优异的果胶诱导效果，特别是初始味觉诱导类型的果胶诱导效果、因此，本发明提供了一种由这种物质组成的角美诱导剂，以及一种由该物质和其他具有 CaSR 激动剂活性的物质组合而成的复合角美诱导剂。更具体地说，本发明提供了一种由 γ -Glu-Abu （L- γ -谷氨酰-L-2-氨基丁酸）组成的角膜诱导剂，以及一种由上述物质和另一种具有 CaSR 激动剂活性的物质组合而成的复合角膜诱导剂。
• YEAST AND YEAST EXTRACT CONTAINING -Glu-Abu, AND METHOD FOR PRODUCING THE YEAST OR YEAST EXTRACT CONTAINING -Glu-Abu
  申请人：Ajinomoto Co., Inc.

  公开号：EP2626430A1

  公开（公告）日：2013-08-14

  A yeast extract containing 0.2% or more of γ-Glu-Abu based on dry weight of the yeast extract is produced by culturing a yeast, such as Saccharomyces cervisiae or Candida utilis, in a medium containing a compound selected from Abu (L-2-aminobutyric acid) and γ-Glu-Abu (L-γ-glutamyl-L-2-aminobutyric acid), and preparing a yeast extract from the obtained cells.

  通过在含有选自 Abu（L-2-氨基丁酸）和 γ-Glu-Abu（L-γ-谷氨酰-L-2-氨基丁酸）的化合物的培养基中培养酵母（如酿酒酵母（Saccharomyces cervisiae）或白色念珠菌（Candida utilis）），并从获得的细胞中制备酵母提取物，制备出以酵母提取物干重为基准、含有 0.2% 或更多 γ-Glu-Abu 的酵母提取物。