mevalonate 5-pyrophosphate | 103025-21-4

• 分子结构分类: 有机化合物 - 有机氧化合物 - 有机含氧阴离子化合物
• 英文名称: mevalonate 5-pyrophosphate
• 英文别名: mevalonate 5-diphosphate；(3r)-3-Hydroxy-5-{[(R)-Hydroxy(Phosphonooxy)phosphoryl]oxy}-3-Methylpentanoic Acid；(3R)-3-hydroxy-5-[hydroxy(phosphonooxy)phosphoryl]oxy-3-methylpentanoic acid
• CAS: 103025-21-4
• 化学式: C₆H₁₄O₁₀P₂
• 分子量: 308.119
• InChiKey: SIGQQUBJQXSAMW-ZCFIWIBFSA-N

物化性质

• 物理描述：
  Solid

计算性质

• 辛醇/水分配系数(LogP)：
  -3.2
• 重原子数：
  18
• 可旋转键数：
  8
• 环数：
  0.0
• sp3杂化的碳原子比例：
  0.83
• 拓扑面积：
  171
• 氢给体数：
  5
• 氢受体数：
  10

反应信息

• 作为反应物：
  描述：

  mevalonate 5-pyrophosphate 在 5’-三磷酸腺苷 作用下， 生成 二磷酸 P-(3-甲基-3-丁烯-1-基)酯
  参考文献：
  名称：

  Mutation and inhibition studies of mevalonate 5-diphosphate decarboxylase

  摘要：

  Mevalonate 5-diphosphate decarboxylase plays an important role in regulating cholesterol biosynthesis, which was studied through incubation with various synthetic substrate analogs and characterization of mutated enzymes. The results are potentially useful for further developing inhibitors that block the mevalonate pathway which is a drug target for treating cardiovascular disease and cancer. (C) 2007 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.bmcl.2007.09.033

文献信息

• Purification and Characterization of Mouse Mevalonate Pyrophosphate Decarboxylase.
  作者：Akihiro Michihara、Kenji Akasaki、Yukio Yamori、Hiroshi Tsuji

  DOI：10.1248/bpb.25.302

  日期：——

  Mevalonate pyrophosphate decarboxylase (MPD) in mouse liver was purified by affinity chromatography. The purified enzyme was a homodimer of 46-kDa subunits and had an isoelectric point of 5.0. Kinetic analysis revealed an apparent Km value of 10 μM for mevalonate pyrophosphate. The enzyme required ATP as a phosphate acceptor and Mg as a divalent cation, which could be substituted with Mn or Co. Its optimum pH was 4.0—7.0. A comparison with MPD from various other sources revealed the mouse MPD to have essentially the same properties as rat MPD, expect for the optimum pH range. An excess of rabbit anti-rat MPD antibody deleted approximately 80% of the MPD activity in the crude extract of mouse liver. These results suggested that the homodimer of 46-kDa subunits represents the major active form of MPD in mice.

  通过亲和层析法纯化了小鼠肝脏中的焦磷酸甲羟戊酸脱羧酶（MPD）。纯化的酶是 46 kDa 亚基的同源二聚体，等电点为 5.0。动力学分析表明，焦磷酸甲羟戊酸的表观 Km 值为 10 μ<小>M。该酶需要 ATP 作为磷酸受体，Mg 作为二价阳离子，可以用 Mn 或 Co 取代。与其他不同来源的 MPD 进行比较后发现，小鼠 MPD 除最适 pH 值范围外，与大鼠 MPD 具有基本相同的特性。过量的兔抗大鼠 MPD 抗体会破坏小鼠肝脏粗提取物中约 80% 的 MPD 活性。这些结果表明，46-kDa 亚基的同源二聚体代表了小鼠 MPD 的主要活性形式。
• Process for the production of fine chemicals
  申请人：Puzio Piotr

  公开号：US20070118916A1

  公开（公告）日：2007-05-24

  The present invention relates to a process for the production of the fine chemical in a microorganism, a plant cell, a plant, a plant tissue or in one or more parts thereof, preferably in plastids. The invention furthermore relates to nucleic acid molecules, polypeptides, nucleic acid constructs, vectors, antibodies, host cells, plant tissue, propagation material, harvested material, plants, microorganisms as well as agricultural compositions and to their use.

  本发明涉及一种在微生物、植物细胞、植物、植物组织或其一部分中生产精细化学品的过程，优选在质体中进行。此外，本发明还涉及核酸分子、多肽、核酸构建物、载体、抗体、宿主细胞、植物组织、繁殖材料、收获材料、植物、微生物以及农业组合物以及它们的用途。
• POLYMERS OF ISROPENE FROM RENEWABLE RESOURCES
  申请人：THE GOODYEAR TIRE & RUBBER COMPANY

  公开号：US20130253141A1

  公开（公告）日：2013-09-26

  It has been found that certain cells in culture can convert more than about 0.002 percent of the carbon available in the cell culture medium into isoprene. These cells have a heterologous nucleic acid that (i) encodes an isoprene synthase polypeptide and (ii) is operably linked to a promoter. The isoprene produced in such a cultured medium can then be recovered and polymerized into synthetic rubbers and other useful polymeric materials. The synthetic isoprene containing polymers of this invention offer the benefit of being verifiable as to being derived from non-petrochemical based resources. They can also be analytically distinguished from rubbers that come from natural sources. The present invention more specifically discloses a polyisoprene polymer which is comprised of repeat units that are derived from isoprene monomer, wherein the polyisoprene polymer has δ 13 C value of greater than −22‰.

  已经发现，在培养细胞中，某些细胞可以将细胞培养基中可用的碳转化为异戊二烯的比例超过0.002％。这些细胞具有异源核酸，它（i）编码异戊二烯合成酶多肽，且（ii）与启动子可操作地连接。在这样的培养基中产生的异戊二烯可以被回收并聚合成合成橡胶和其他有用的高分子材料。本发明的合成异戊二烯含聚合物具有从非石化资源衍生的可验证性的优点。它们也可以从天然来源的橡胶中进行分析区分。本发明更具体地披露了一种聚异戊二烯聚合物，其由异戊二烯单体衍生的重复单位组成，其中聚异戊二烯聚合物的δ13C值大于-22‰。
• New genes related to a process for the production of fine chemicals
  申请人：Metanomics GmbH

  公开号：EP1953235A2

  公开（公告）日：2008-08-06

  The present invention relates to a process for the production of the fine chemical in a microorganism, a plant cell, a plant, a plant tissue or in one or more parts thereof. The invention furthermore relates to nucleic acid molecules, polypeptides, nucleic acid constructs, vectors, antisense molecules, antibodies, host cells, plant tissue, propagation material, harvested material, plants, microorganisms as well as agricultural compositions and to their use.

  本发明涉及一种在微生物、植物细胞、植物、植物组织或其一个或多个部分中生产精细化学品的工艺。本发明还涉及核酸分子、多肽、核酸构建体、载体、反义分子、抗体、宿主细胞、植物组织、繁殖材料、收获材料、植物、微生物以及农业组合物及其用途。
• Process for the production of lutein
  申请人：Metanomics GmbH

  公开号：EP2096177A2

  公开（公告）日：2009-09-02

  The present invention relates further to a process for the control of the production of fine chemical in a microorganism, a plant cell, a plant, a plant tissue or in one or more parts thereof. The invention furthermore relates to nucleic acid molecules, polypeptides, nucleic acid constructs, vectors, antisense molecules, antibodies, host cells, plant tissue, propagation material, harvested material, plants, microorganisms as well as agricultural compositions and to their use.

  本发明还涉及一种控制微生物、植物细胞、植物、植物组织或其一个或多个部分生产精细化学品的工艺。本发明还涉及核酸分子、多肽、核酸构建体、载体、反义分子、抗体、宿主细胞、植物组织、繁殖材料、收获材料、植物、微生物以及农业组合物及其用途。