L-N^ε-(Ethoxycarbonyl)lysine | 5701-16-6

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 英文名称: L-N^ε-(Ethoxycarbonyl)lysine
• 英文别名: N-Carbethoxy-L-lysine, epsilon-；(2S)-2-amino-6-(ethoxycarbonylamino)hexanoic acid
• CAS: 5701-16-6
• 化学式: C₉H₁₈N₂O₄
• 分子量: 218.253
• InChiKey: XMVZHEKLZHYPQS-ZETCQYMHSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -2.2
• 重原子数：
  15
• 可旋转键数：
  8
• 环数：
  0.0
• sp3杂化的碳原子比例：
  0.78
• 拓扑面积：
  102
• 氢给体数：
  3
• 氢受体数：
  5

反应信息

• 作为反应物：
  描述：

  L-N^ε-(Ethoxycarbonyl)lysine 在 盐酸 、 sodium hydroxide 、 正丁基锂 作用下， 反应 8.0h， 生成
  参考文献：
  名称：

  .alpha.-Amino acids as chiral educts for asymmetric products. Aminoacylation of metallo alkyls and alkenyls

  摘要：

  DOI：

  10.1021/jo00161a023
• 作为产物：
  描述：

  L-赖氨酸盐酸盐 、 氯甲酸乙酯 在 copper(II) carbonate 作用下， 生成 L-N^ε-(Ethoxycarbonyl)lysine
  参考文献：
  名称：

  .alpha.-Amino acids as chiral educts for asymmetric products. Aminoacylation of metallo alkyls and alkenyls

  摘要：

  DOI：

  10.1021/jo00161a023

文献信息

• ACTIVATABLE CRISPR/CAS9 FOR SPATIAL AND TEMPORAL CONTROL OF GENOME EDITING
  申请人：University of Pittsburgh - Of the Commonwealth System of Higher Education

  公开号：US20180073002A1

  公开（公告）日：2018-03-15

  Disclosed herein is a genetically encoded light- or chemically-activated Cas9 engineered through the site-specific installation of an activatable lysine amino acid. Such activatable Cas9 proteins can be used in CRISPR/Cas9 systems to control gene expression temporally, spatially, or both. Systems, methods, kits, and compositions for manipulation of sequences and/or activities of target sequences are provided. Also provided are methods of directing CRISPR complex formation in cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas9 system.

  本文公开了一种通过位点特异性安装可活化赖氨酸氨基酸而工程化的基因编码光或化学激活的Cas9。这种可活化的Cas9蛋白质可以用于CRISPR/Cas9系统中暂时或空间地控制基因表达。本文还提供了用于操作目标序列的序列和/或活性的系统、方法、工具包和组合物。还提供了在细胞中指导CRISPR复合物形成的方法以及利用CRISPR/Cas9系统引入精确突变选择特定细胞的方法。
• Activatable CRISPR/CAS9 for spatial and temporal control of genome editing
  申请人：University of Pittsburgh—Of the Commonwealth System of Higher Education

  公开号：US11214779B2

  公开（公告）日：2022-01-04

  Disclosed herein is a genetically encoded light- or chemically-activated Cas9 engineered through the site-specific installation of an activatable lysine amino acid. Such activatable Cas9 proteins can be used in CRISPR/Cas9 systems to control gene expression temporally, spatially, or both. Systems, methods, kits, and compositions for manipulation of sequences and/or activities of target sequences are provided. Also provided are methods of directing CRISPR complex formation in cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas9 system.

  本文公开了一种基因编码的光激活或化学激活 Cas9，它是通过在特定位点安装可激活的赖氨酸氨基酸而设计的。这种可激活的 Cas9 蛋白可用于 CRISPR/Cas9 系统，以控制基因表达的时间、空间或两者。本文提供了用于操纵目标序列的序列和/或活性的系统、方法、试剂盒和组合物。此外，还提供了在细胞中引导 CRISPR 复合物形成的方法，以及通过利用 CRISPR/Cas9 系统引入精确突变来选择特定细胞的方法。
• AMINOACYL-TRNA SYNTHETASE FOR EFFICIENTLY INTRODUCING LYSINE DERIVATIVE IN PROTEIN
  申请人：NINGBO KUNPENG BIOTECH CO., LTD.

  公开号：US20220170030A1

  公开（公告）日：2022-06-02

  Provided is a mutant lysyl-tRNA synthetase. Compared with a wild-type lysyl-tRNA synthetase, the mutant lysyl-tRNA synthetase of the present invention can increase the amount of non-natural amino acids inserted and the amount of a protein of interest containing non-natural amino acids, and can also improve the stability of the protein of interest, thereby making same less prone to fragmentation.
• .alpha.-Amino acids as chiral educts for asymmetric products. Aminoacylation of metallo alkyls and alkenyls
  作者：Christopher G. Knudsen、Henry Rapoport

  DOI：10.1021/jo00161a023

  日期：1983.7