4-{4,5-Bis[4-(dimethylamino)phenyl]-1,3-dihydro-2H-imidazol-2-ylidene}cyclohexa-2,5-dien-1-one | 64486-60-8

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 唑类
• 英文名称: 4-{4,5-Bis[4-(dimethylamino)phenyl]-1,3-dihydro-2H-imidazol-2-ylidene}cyclohexa-2,5-dien-1-one
• 英文别名: 4-[4,5-bis[4-(dimethylamino)phenyl]-1H-imidazol-2-yl]phenol
• CAS: 64486-60-8
• 化学式: C₂₅H₂₆N₄O
• 分子量: 398.5
• InChiKey: QTIGCKVHRYBEKI-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  4.9
• 重原子数：
  30
• 可旋转键数：
  5
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.16
• 拓扑面积：
  55.4
• 氢给体数：
  2
• 氢受体数：
  4

文献信息

• Dye-providing composition, diagnostic test kit and their use in method for ligand determination using peroxidase labeled-receptor
  申请人：Johnson & Johnson Clinical Diagnostics, Inc.

  公开号：EP0308236A2

  公开（公告）日：1989-03-22

  A dye-providing composition comprises a water-soluble or -dispersible polymer, such as a vinylpyrrolidone polymer, and an imidazole leuco dye capable of providing a dye in the presence of hydrogen peroxide and a peroxidative substance. The weight ratio of polymer to leuco dye is from 10,000:1 to 100:1. The dye-providing composition can be included with a peroxidase substrate in a diagnostic test kit. A method for the determination of a ligand can be carried out using a peroxidase labeled-receptor for the ligand and the dye-providing composition described above. The method is particularly useful for the determination of human chorionic gonadotropin.

  一种提供染料的组合物由水溶性或可分散性聚合物（如乙烯基吡咯烷酮聚合物）和在过氧化氢和过氧化物存在下能提供染料的咪唑白胺染料组成。聚合物与白兰地染料的重量比为 10,000:1 至 100:1。提供染料的组合物可与过氧化物酶底物一起加入诊断试剂盒中。使用过氧化物酶标记的配体受体和上述提供染料的组合物，可以进行配体的测定方法。该方法尤其适用于测定人绒毛膜促性腺激素。
• Imidazole leuco dye composition containing 4'-hydroxyacetanilide diagnostic kit and method using same
  申请人：EASTMAN KODAK COMPANY (a New Jersey corporation)

  公开号：EP0347139A2

  公开（公告）日：1989-12-20

  A dye-providing composition is useful in various diagnostic assays wherein a peroxidase-­labeled specific binding species is used. This composition is substantially free of peroxidase and such labeled species, and comprises an imidazole leuco dye and 4′-hydroxyacetanilide present in an amount up to 2.5 mmolar. This composition can be included as part of a diagnostic test kit.

  一种提供染料的组合物适用于使用过氧化物酶标记的特异性结合物种的各种诊断化验。这种组合物基本上不含过氧化物酶和此类标记物，由咪唑类白兰地染料和 4′-羟基乙酰苯胺组成，其含量可达 2.5 毫摩尔。这种组合物可作为诊断检测试剂盒的一部分。
• Buffered wash composition, insolubilizing composition, test kits and method of use
  申请人：Johnson & Johnson Clinical Diagnostics, Inc.

  公开号：EP0431682A2

  公开（公告）日：1991-06-12

  A buffered aqueous composition is useful simultaneously as a wash solution and a dye-providing composition in specific binding assays involving enzyme-labeled specific binding reagents. The wash composition includes a dye-providing composition, a buffer and an organic solvent having a certain molecular weight and water-solubility. Another useful composition includes a particulate substrate having avidin attached thereto, and a peroxidase reducing agent. Either composition can be provided in a diagnostic test kit, and can be used to detect a specific binding ligand in assays.

  在涉及酶标记的特异性结合试剂的特异性结合检测中，缓冲水溶液组合物可同时用作洗涤液和供染组合物。洗涤组合物包括染料提供组合物、缓冲液和具有一定分子量和水溶性的有机溶剂。另一种有用的组合物包括附有阿维丁的颗粒基质和过氧化物酶还原剂。这两种组合物都可以在诊断检测试剂盒中提供，并可用于在检测中检测特定的结合配体。 特异性结合配体。
• Use of 4' of 5'-hydroxy and 4'- or 5'-alkoxy-substituted electron transfer agents in compositions, elements, test kits and analytical methods
  申请人：Johnson & Johnson Clinical Diagnostics, Inc.

  公开号：EP0603953A1

  公开（公告）日：1994-06-29

  Aqueous compositions, test kits, test devices and methods can be used to detect hydrogen peroxide or peroxidase by generating a colorimetric or chemiluminescent signal in the presence of the analyte. Signal generation is enhanced by the presence of certain substituted 4-hydroxy- or 4-alkoxy-substituted phenyl or naphthyl electron transfer agents.

  水性组合物、检测试剂盒、检测设备和方法可用于检测过氧化氢或过氧化物酶，在被分析物存在的情况下产生比色或化学发光信号。某些取代的 4-羟基或 4-烷氧基取代的苯基或萘基电子转移剂的存在可增强信号的产生。
• Analytical element and immunoassay using a 4-hydroxy or 4-alkoxyarylacetamide as stabilizer
  申请人：Johnson & Johnson Clinical Diagnostics, Inc.

  公开号：EP0603954A2

  公开（公告）日：1994-06-29

  A dry analytical element can be used to sensitively and rapidly detect a wide variety of specific binding ligands in either a competitive binding or sandwich assay format. The assays are carried out using a peroxidase-labeled immunoreactant. The peroxidase label is stabilized with a 4-hydroxy or 4-alkoxyarylacetamide which is located in one or more zones of the element. Not only is the label stabilized with the stabilizer, but the assay is more sensitive.

  干式分析元件可用于以竞争性结合或夹心检测的形式灵敏、快速地检测各种特异性结合配体。检测使用过氧化物酶标记的免疫反应物。过氧化物酶标签用 4-羟基或 4-烷氧基芳基乙酰胺稳定，这种乙酰胺位于元件的一个或多个区域。使用稳定剂不仅可以稳定标签，还能提高检测灵敏度。