2,2'-dihydroxy-5,5'-bis(ethylamino)diphenyl | 4542-40-9

• 分子结构分类: 有机化合物 - 苯类化合物 - 苯和取代衍生物
• 英文名称: 2,2'-dihydroxy-5,5'-bis(ethylamino)diphenyl
• 英文别名: dityramine；5,5'-bis-(2-amino-ethyl)-biphenyl-2,2'-diol；5,5'-Bis-(2-amino-aethyl)-biphenyl-2,2'-diol；4-(2-Aminoethyl)-2-[5-(2-aminoethyl)-2-hydroxyphenyl]phenol
• CAS: 4542-40-9
• 化学式: C₁₆H₂₀N₂O₂
• 分子量: 272.347
• InChiKey: SYQCMEMKJHAVBA-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  1.5
• 重原子数：
  20
• 可旋转键数：
  5
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.25
• 拓扑面积：
  92.5
• 氢给体数：
  4
• 氢受体数：
  4

反应信息

• 作为反应物：
  描述：

  2,2'-dihydroxy-5,5'-bis(ethylamino)diphenyl 、 硫酸二甲酯 在 sodium hydroxide 作用下， 生成 2,2'-dimethoxy-5,5'-bis-(2-trimethylammonio-ethyl)-biphenyl; bis-methyl sulfate
  参考文献：
  名称：

  Thermally stressed state of an ellipsoidal inhomogeneity (inclusion) in the case of polynomial external force and temperature fields

  摘要：

  DOI：

  10.1007/bf02674049
• 作为产物：
  描述：

  N-乙酰基酪胺 在 盐酸 、 双氧水 、 horseradish peroxidase 作用下， 以 sodium hydroxide 为溶剂， 反应 48.0h， 生成 2,2'-dihydroxy-5,5'-bis(ethylamino)diphenyl
  参考文献：
  名称：

  Evidence of a coupled mechanism between monoamine oxidase and peroxidase in the metabolism of tyramine by rat intestinal mitochondria

  摘要：

  The relationship between monoamine oxidase (EC 1.4.3.4; MAO) and peroxidase (EC 1.11.1.7; POD) in the metabolism of tyramine was investigated using the crude mitochondrial fraction of rat intestine. When tyramine was incubated with mitochondria, the formation of the peroxidase-catalysed oxidation product, 2,2'-dihydroxy-5,5'-bis(ethylamino)diphenyl (dityramine), identified by mass spectrometric analysis, was monitored spectrophotometrically. After an initial lag time, the formation rate of dityramine was linear up to 2 hr, amounting to 17 nmol X hr(-1) X mg protein(-1). A similar value was found for the oxidative deamination of tyramine catalysed by intestinal MAO. Either 10(-3) M clorgyline or 10(-3) M NaCN suppressed this reaction by completely inhibiting MAO or POD, respectively. In the former case, however, addition of H2O2 to the incubation mixture promptly started the reaction. Selective inhibition of MAO-A and MAO-B was achieved with 3 X 10(-7) M clorgyline and 3 X 10(-7) M deprenyl, respectively, and the formation rate of dityramine decreased in a corresponding manner. Preincubation with histamine or spermidine reduced the lag time without affecting the steady-state reaction rate. Higher levels of dityramine were also detected in vivo in rat intestine after oral administration of tyramine. These results indicate that the peroxidase-dependent metabolism of tyramine in the gut may be driven by H2O2 produced by MAO activities and that MAO-A is mainly responsible for this process, as well as for the oxidative deamination of tyramine. (C) 1998 Elsevier Science Inc.

  DOI：

  10.1016/s0006-2952(97)00379-1

文献信息

• Regulative peroxidase activity of DNA-linked hemin by graphene oxide for fluorescence DNA sensing
  作者：Quanbo Wang、Nan Xu、Jianping Lei、Huangxian Ju

  DOI：10.1039/c4cc02273d

  日期：——

  The inhibition effect of graphene oxide toward the peroxidase activity of DNA-linked hemin was identified for fluorescence DNA sensing.

  翻译结果：鉴于荧光DNA传感，氧化石墨烯对DNA-血红素连接的过氧化物酶活性的抑制作用被确定。
• Exploitation of Luminescent Lanthanide Nanoparticles for a Sensitivity-Enhanced ELISA Detection Method
  作者：Ali A. Kassir、Clémence Cheignon、Loïc J. Charbonnière

  DOI：10.1021/acs.analchem.3c04821

  日期：2024.2.6

  on the photoluminescence properties of dye-sensitized lanthanide nanoparticles (Ln NPs) was developed for enzyme-linked immunosorbent assays (ELISAs). In this method, the horseradish peroxidase (HRP) enzyme catalyzes the oxidation of phenol derivatives in the presence of hydrogen peroxide, providing dimers that are able to interact with the Ln NP surface and to efficiently photosensitize the Ln ions

  开发了一种基于染料敏化稀土纳米颗粒（Ln NP）光致发光特性的新检测方法，用于酶联免疫吸附测定（ELISA）。在该方法中，辣根过氧化物酶 (HRP) 在过氧化氢存在下催化苯酚衍生物的氧化，提供能够与 Ln NP 表面相互作用并有效光敏化 Ln 离子的二聚体。由于 Ln 的发射寿命非常长，Ln NP 发光的时间选通检测可以消除生物环境引起的背景噪声。在对各种苯酚衍生物的酶催化氧化进行比较后，选择 4-羟基苯乙酸甲酯 (MHPA) 作为最有前途的底物，因为在其 HRP 催化氧化后观察到最高的 Ln 发射强度。经过对酶反应和 Ln 敏化条件（缓冲液、pH、反应物浓度、NP 类型等）的精心优化，这种新的检测方法成功应用于商业胰岛素 ELISA 试剂盒，作为证明-与商业检测方法相比，其灵敏度更高。
• Method of gene mapping
  申请人：E.I. DU PONT DE NEMOURS AND COMPANY

  公开号：EP0309969A2

  公开（公告）日：1989-04-05

  The method described characterizes each DNA segment to be mapped by cleaving it to produce DNA fragments which are then end labeled with a reporter(s) specific to the end nucleotides of each fragment. The labeled fragments are again cleaved to produce short fragments which are separated according to size. The short fragments are analyzed as to reporter identity and size which is indicative of the character of each fragment. By derivatizing the cleaved ends of the primary cleaved fragments, the labeling may be delayed until the second cleavage. Prior to labeling the derivatized fragments, all underivatized fragments are removed, the derivatized fragments being immobilized.

  所述方法通过裂解 DNA 片段产生 DNA 片段，然后用特异于每个片段末端核苷酸的报告物对其进行末端标记，从而确定要绘制的每个 DNA 片段的特征。标记后的片段再次裂解，产生短片段，并根据大小将其分离。对短片段进行分析，以确定报告基因的特性和大小，这表明了每个片段的特性。通过对初级裂解片段的裂解末端进行衍生处理，可将标记延迟到第二次裂解。在对衍生片段进行标记之前，先移除所有未充分活化的片段，然后固定衍生片段。
• Polynucleotide hybridization assays employing catalyzed luminescence
  申请人：E.I. DU PONT DE NEMOURS AND COMPANY

  公开号：EP0185547B1

  公开（公告）日：1992-06-03
• Process and reagents for DNA sequence analysis
  申请人：E.I. DU PONT DE NEMOURS AND COMPANY

  公开号：EP0252683B1

  公开（公告）日：1995-01-18