7-Butyryloxy-3-carboxymethylaminocarbonyl-6-chlorocoumarin | 183736-49-4

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 英文名称: 7-Butyryloxy-3-carboxymethylaminocarbonyl-6-chlorocoumarin
• 英文别名: 2-[(7-butanoyloxy-6-chloro-2-oxochromene-3-carbonyl)amino]acetic acid
• CAS: 183736-49-4
• 化学式: C₁₆H₁₄ClNO₇
• 分子量: 367.743
• InChiKey: VDVCLQZKFZSLPM-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  2.7
• 重原子数：
  25
• 可旋转键数：
  7
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.25
• 拓扑面积：
  119
• 氢给体数：
  2
• 氢受体数：
  7

反应信息

• 作为产物：
  描述：

  7-butyryloxy-3-benzyloxycarbonylmethylaminocarbonyl-6-chlorocoumarin 在 钯 溶剂黄146 作用下， 以 1,4-二氧六环 、 chloroform-absolute ethanol 、 甲苯 为溶剂， 生成 7-Butyryloxy-3-carboxymethylaminocarbonyl-6-chlorocoumarin 、 5-氯-2,4-二羟基苯甲醛
  参考文献：
  名称：

  Promiscuous G-protein compositions and their use

  摘要：

  揭示了用于识别G蛋白偶联受体、配体以及调节信号转导的化合物的组合物和使用它们的方法。这些组合物和方法利用了多功能G蛋白。可以通过各种测定方法检测多功能G蛋白的激活，包括在激活导致含有该组合物的样品的荧光发射变化的测定中。

  公开号：

  US20060008855A1

文献信息

• Detection of transmembrane potentials by optical methods
  申请人：——

  公开号：US20030207248A1

  公开（公告）日：2003-11-06

  Methods and compositions are provided for detecting changes in membrane potential in membranes biological systems. In one aspect, the method comprises; a) providing a living cell with a first reagent comprising a charged hydrophobic molecule which is typically a fluorescence resonance energy transfer (FRET) acceptor or donor, or is a quencher and is capable of redistributing within the membrane of a biological membrane in response to changes in the potential across the membrane; b) providing the cell with a second reagent that can label the first face or the second face of a biological membrane within the cell; c) detecting light emission from the first reagent or the second reagent. One aspect of this method involves monitoring membrane potential changes in subcellular organelle membranes in a living cells. Another aspect of the invention is the use of certain embodiments of the method for the screening of test chemicals for activity to modulate the activity of a target ion channel. Another aspect of the present invention is a transgenic organism comprising a first reagent that comprises a charged hydrophobic fluorescent molecule, and a second reagent comprising a bioluminescent or naturally fluorescent protein.

  提供了一种检测生物系统膜电位变化的方法和组合物。在一个方面，该方法包括：a）提供一种带有带电疏水分子的第一试剂给活细胞，该带电疏水分子通常是荧光共振能量转移（FRET）受体或给体，或者是淬灭剂，并能够在生物膜跨膜电势变化时在生物膜内重新分布；b）向细胞提供可以标记细胞内生物膜的第一面或第二面的第二试剂；c）检测第一试剂或第二试剂的光发射。该方法的一个方面涉及在活细胞中监测亚细胞器膜的膜电位变化。发明的另一个方面是使用该方法的某些实施方式筛选测试化学物质以调节目标离子通道的活性。本发明的另一个方面是包括第一试剂，其中包含带电疏水荧光分子的转基因生物体，以及包含生物发光蛋白或天然荧光蛋白的第二试剂。
• DETECTION OF TRANSMEMBRANE POTENTIALS BY OPTICAL METHODS
  申请人：——

  公开号：US20020137201A1

  公开（公告）日：2002-09-26

  Methods and compositions are provided for determining the potential of a membrane. In one aspect, the method comprises: (a) introducing a first reagent comprising a hydrophobic fluorescent ion capable of redistributing from a first face of the membrane to a second face of the membrane in response to changes in the potential of the membrane, as described by the Nernst equation, (b) introducing a second reagent which labels the first face or the second face of the membrane, which second reagent comprises a chromophore capable of undergoing energy transfer by either (i) donating excited state energy to the fluorescent ion, or (ii) accepting excited state energy from the fluorescent ion, (c) exposing the membrane to radiation; (d) measuring energy transfer between the fluorescent ion and the second reagent, and (e) relating the energy transfer to the membrane potential. Energy transfer is typically measured by fluorescence resonance energy transfer. In some embodiments the first and second reagents are bound together by a suitable linker. In one aspect the method is used to identify compounds which modulate membrane potentials in biological membranes.

  提供了一种测定膜电位潜力的方法和组合物。在一个方面，该方法包括：(a)引入第一试剂，该试剂包括一种疏水性荧光离子，能够根据Nernst方程式的描述从膜的第一面重新分布到膜的第二面，以响应膜电位的变化，(b)引入第二试剂，该试剂标记膜的第一面或第二面，该试剂包括一种染色体，能够通过(i)将激发态能量转移给荧光离子，或(ii)从荧光离子接受激发态能量，(c)将膜暴露于辐射下，(d)测量荧光离子和第二试剂之间的能量转移，(e)将能量转移与膜电位相关联。能量转移通常通过荧光共振能量转移来测量。在某些实施例中，第一和第二试剂由适当的连接剂结合在一起。在一个方面，该方法用于鉴定调节生物膜电位的化合物。
• Cytosolic forms for &bgr;-lactamase and uses thereof
  申请人：The Regents of the University of California

  公开号：US06472205B1

  公开（公告）日：2002-10-29

  The present invention is directed to nucleic acid molecules that encode a cytosolic form of beta-lactamase, cells that include such nucleic acid molecules and methods for their use. These cells can be used in a variety of methods, such as methods for monitoring the expression of a gene or biochemical activity.

  本发明涉及编码细胞质型β-内酰胺酶的核酸分子，包含此类核酸分子的细胞以及其使用方法。这些细胞可用于多种方法，例如用于监测基因表达或生化活性的方法。
• Substrates for beta-lactamase and uses thereof
  申请人：Tsien Y. Roger

  公开号：US20070184513A1

  公开（公告）日：2007-08-09

  Substrates for β-lactamase of the general formula I in which one of X and Y is a fluorescent donor moiety and the other is a quencher (which may or may not re-emit); R′ is selected from the group consisting of H, lower (i.e., alkyl of 1 to about 5 carbon atoms) and (CH 2 ) n OH, in which n is 0 or an integer from 1 to 5; R″ is selected from the group consisting of H, physiologically acceptable metal and ammonium cations, CHR 2 OCO(CH 2 ) n CH 3 , —CHR 2 OCOC (CH 3 ) 3 , acylthiomethyl, acyloxy-alpha -benzyl, delta-butyrolactonyl, methoxycarbonyloxymethyl, phenyl, methylsulphinylmethyl, beta-morpholinoethyl, dialkylaminoethyl, acyloxyalkyl, dialkylaminocarbonyloxymethyl and aliphatic, in which R 2 is selected from the group consisting of H and lower alkyl; A is selected from the group consisting of S, O, SO, SO 2 and CH 2 ; and Z′ and Z″ are linkers for the fluorescent donor and quencher moieties. Methods of assaying β-lactamase activity and monitoring expression in systems using β-lactamase as a reporter gene also are disclosed.

  通式I的β-内酰胺酶底物，其中X和Y中的一个是荧光给体基团，另一个是猝灭剂（可以或不可以再发射）；R'从H，较低的（即1到约5个碳原子的烷基）和（CH2）nOH中选择，其中n为0或1到5的整数；R"从H，生理上可接受的金属和铵阳离子，CHR2OCO（CH2）nCH3，—CHR2OCOC（CH3）3，酰硫甲基，酰氧-α-苯甲基，δ-丁酰内酯，甲氧羰氧甲基，苯基，甲硫氧甲基，β-吗啡啉乙基，二烷基氨基乙基，酰氧烷基，二烷基氨基羰氧甲基和脂肪族中选择，其中R2从H和较低的烷基中选择；A从S，O，SO，SO2和CH2中选择；Z'和Z"是荧光给体和猝灭剂基团的连接器。还公开了评估β-内酰胺酶活性和使用β-内酰胺酶作为报告基因监测表达的方法。
• Substrates for .beta.-lactamase and uses thereof
  申请人：The Regents of the University of California

  公开号：US05955604A1

  公开（公告）日：1999-09-21

  Substrates for .beta.-lactamase of the general formula I ##STR1## in which one of X and Y is a fluorescent donor moiety and the other is a quencher (which may or may not re-emit); R' is selected from the group consisting of H, lower (i.e., alkyl of 1 to about 5 carbon atoms) and (CH.sub.2).sub.n OH, in which n is 0 or an integer from 1 to 5; R" is selected from the group consisting of H, physiologically acceptable metal and ammonium cations, --CHR.sup.2 OCO(CH.sub.2).sub.n CH.sub.3, --CHR.sup.2 OCOC(CH.sub.3).sub.3, acylthiomethyl, acyloxy-alpha-benzyl, delta-butyrolactonyl, methoxycarbonyloxymethyl, phenyl, methylsulphinylmethyl, beta-morpholinoethyl, dialkylaminoethyl, acyloxyalkyl, dialkylaminocarbonyloxymethyl and aliphatic, in which R.sup.2 is selected from the group consisting of H and lower alkyl; A is selected from the group consisting of S, O, SO, SO.sub.2 and CH.sub.2 ; and Z' and Z" are linkers for the fluorescent donor and quencher moieties. Methods of assaying .beta.-lactamase activity and monitoring expression in systems using .beta.-lactamase as a reporter gene also are disclosed.

  通式I的β-内酰胺酶底物为：##STR1## 其中X和Y中的一个是荧光供体基团，另一个是猝灭剂（可以或不可以再发射）；R'从H，较低的（即1至约5个碳原子的烷基）和（CH.sub.2).sub.n OH中选择，其中n为0或整数1至5；R"从H，生理上可接受的金属和铵阳离子，--CHR.sup.2 OCO(CH.sub.2).sub.n CH.sub.3，--CHR.sup.2 OCOC(CH.sub.3).sub.3，酰硫甲基，酰氧基-α-苯甲基，δ-丁酰内酯，甲氧羰氧甲基，苯基，甲磺酰甲基，β-吗啡基乙基，二烷基氨基乙基，酰氧基烷基，二烷基氨基羰氧甲基和脂肪族中选择，其中R.sup.2从H和较低的烷基中选择；A从S，O，SO，SO.sub.2和CH.sub.2中选择；Z'和Z"是荧光供体和猝灭剂基团的连接剂。还公开了测定β-内酰胺酶活性和使用β-内酰胺酶作为报告基因的系统中监测表达的方法。