4'-Methyl-N2-(pyrimidin-2-yl)-[4,5'-bithiazole]-2,2'-diamine

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 唑类
• 英文名称: 4'-Methyl-N2-(pyrimidin-2-yl)-[4,5'-bithiazole]-2,2'-diamine
• 英文别名: 4-methyl-5-[2-(pyrimidin-2-ylamino)-1,3-thiazol-4-yl]-1,3-thiazol-2-amine
• 化学式: C₁₁H₁₀N₆S₂
• 分子量: 290.4
• InChiKey: FHVNBKUGDCJKHS-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  2.2
• 重原子数：
  19
• 可旋转键数：
  3
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.09
• 拓扑面积：
  146
• 氢给体数：
  2
• 氢受体数：
  8

文献信息

• Method to detect activity of a polymerase
  申请人：MAX-PLANCK-GESELLSCHAFT ZUR FÖRDERUNG DER WISSENSCHAFTEN E.V.

  公开号：US10883137B2

  公开（公告）日：2021-01-05

  The present invention relates to methods for detection of nucleotide polymerase activity and methods of detecting compounds that modulate nucleotide polymerase activity, by detecting product formation of the nucleotide polymerase to be tested based on determination of close proximity of two labeled nucleotide probes able to bind the product of the nucleotide polymerase. It is preferred that proximity dependent energy transfer, such as forster resonance energy transfer, between said labeled nucleotide probes is determined. The invention further provides kits comprising components for carrying out the inventive methods for detection of nucleotide polymerase activity.

  本发明涉及核苷酸聚合酶活性的检测方法和调节核苷酸聚合酶活性的化合物的检测方法，其方法是根据能够结合核苷酸聚合酶产物的两个标记核苷酸探针的近距离测定来检测待测核苷酸聚合酶的产物形成。最好是确定所述标记的核苷酸探针之间的能量转移（如福斯特共振能量转移）与距离有关。本发明进一步提供了试剂盒，其中包括用于实施本发明核苷酸聚合酶活性检测方法的组件。
• METHOD TO DETECT ACTIVITY OF A POLYMERASE
  申请人：Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.

  公开号：EP3303613A1

  公开（公告）日：2018-04-11
• [EN] METHOD TO DETECT ACTIVITY OF A POLYMERASE<br/>[FR] PROCÉDÉ DE DÉTECTION DE L'ACTIVITÉ D'UNE POLYMÉRASE
  申请人：MAX-PLANCK-GESELLSCHAFT ZUR FÖRDERUNG DER WSS E V

  公开号：WO2016193231A1

  公开（公告）日：2016-12-08

  The present invention relates to methods for detection of nucleotide polymerase activity and methods of detecting compounds that modulate nucleotide polymerase activity, by detecting product formation of the nucleotide polymerase to be tested based on determination of close proximity of two labeled nucleotide probes able to bind the product of the nucleotide polymerase. It is preferred that proximity dependent energy transfer, such as forster resonance energy transfer, between said labeled nucleotide probes is determined. The invention further provides kits comprising components for carrying out the inventive methods for detection of nucleotide polymerase activity.