propene-1,2,3-tricarboxylic acid-2-methyl ester | 65146-86-3

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 英文名称: propene-1,2,3-tricarboxylic acid-2-methyl ester
• 英文别名: Propen-1,2,3-tricarbonsaeure-2-methylester；2-Methyl-cis-aconitic acid；(Z)-3-methoxycarbonylpent-2-enedioic acid
• CAS: 65146-86-3
• 化学式: C₇H₈O₆
• 分子量: 188.137
• InChiKey: BRYKYSQCLNCYQW-RQOWECAXSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -0.6
• 重原子数：
  13
• 可旋转键数：
  5
• 环数：
  0.0
• sp3杂化的碳原子比例：
  0.29
• 拓扑面积：
  101
• 氢给体数：
  2
• 氢受体数：
  6

反应信息

• 作为反应物：
  描述：

  propene-1,2,3-tricarboxylic acid-2-methyl ester 、 乙酸酐 、 溶剂黄146 生成 alkaline earth salt of/the/ methylsulfuric acid
  参考文献：
  名称：

  Malachowski; Giedroyc; Jerzmanowska, Chemische Berichte, 1928, vol. 61, p. 2525

  摘要：

  DOI：

文献信息

• Probe compound for detecting and isolating enzymes and means and methods using the same
  申请人：Helmholtz-Zentrum für Infektionsforschung GmbH

  公开号：EP2230312A1

  公开（公告）日：2010-09-22

  The present invention relates to a probe compound that can comprise any substrate or metabolite of an enzymatic reaction in addition to an indicator component, such as, for example, a fluorescence dye, or the like. Moreover, the present invention relates to means for detecting enzymes in form of an array, which comprises any number of probe compounds of the invention which each comprise a different metabolite of interconnected metabolites representing the central pathways in all forms of life. Moreover, the present invention relates to a method for detecting enzymes involving the application of cell extracts or the like to the array of the invention which leads to reproducible enzymatic reactions with the substrates. These specific enzymatic reactions trigger the indicator (e.g. a fluorescence signal) and bind the enzymes to the respective cognate substrates. Moreover, the invention relates to means for isolating enzymes in form of nanoparticles coated with the probe compound of the invention. The immobilisation of the cognate substrates or metabolites on the surface of nanoparticles by means of the probe compounds allows capturing and isolating the respective enzyme, e.g. for subsequent sequencing.

  本发明涉及一种探针化合物，它可以包括酶反应的任何底物或代谢物，此外还包括指示成分，例如荧光染料或类似物。此外，本发明还涉及以阵列形式检测酶的方法，该阵列由任意数量的本发明探针化合物组成，每种探针化合物由代表所有生命形式中中心途径的相互关联的代谢物中的不同代谢物组成。此外，本发明还涉及一种检测酶的方法，该方法涉及将细胞提取物或类似物应用于本发明的阵列，从而导致与底物发生可重复的酶反应。这些特定的酶反应会触发指示剂（如荧光信号），并将酶与各自的同源底物结合。此外，本发明还涉及以涂覆有本发明探针化合物的纳米颗粒形式分离酶的方法。通过探针化合物将同源底物或代谢物固定在纳米颗粒表面，可以捕获和分离相应的酶，例如用于后续测序。
• ITACONIC ACID AND ITACONATE METHYLESTER PRODUCTION
  申请人：DSM IP Assets B.V.

  公开号：EP2922952A2

  公开（公告）日：2015-09-30
• RECOMBINANT CELLS PRODUCING ITACONIC ACID AND METHYL ESTERS THEREOF
  申请人：DSM IP Assets B.V.

  公开号：US20170191091A1

  公开（公告）日：2017-07-06

  The present invention relates to a recombinant cell which is capable of producing one or more of itaconic acid, 4-methyl itaconate or 1-methyl itaconate, wherein the said recombinant microorganism has been modified in its genome such that it results in a deficiency in the production of a trans-aconitate methyltransferase. A recombinant yeast cell which is capable of producing itaconic acid and which overexpresses: —a nucleic acid encoding a polypeptide having cis-aconitate decarboxylase activity; and —one or more nucleic acids encoding polypeptides which separately or together catalyze a reaction towards acetyl CoA, wherein the said recombinant microorganism has been modified in its genome such that it results in a deficiency in the production of a trans-aconitate methyltransferase. These recombinant yeast cells may be used in processes for the production of itaconic acid, 4-methyl itaconate or 1-methyl itaconate.
• ITACONIC ACID AND ITACONATE METHYLESTER AND DIMETHYLESTER PRODUCTION
  申请人：DSM IP Assets B.V.

  公开号：US20170191089A1

  公开（公告）日：2017-07-06

  The present invention relates to a recombinant yeast cell which is capable of producing one or more of 4-methyl itaconate, 1-methyl itaconate or 1,4-dimethyl itaconate. The invention also relates to a recombinant yeast cell which is capable of producing itaconic acid and which overexpresses: —a nucleic acid encoding a polypeptide having cis-aconitate decarboxylase activity; and —a nucleic acid encoding a polypeptide which catalyzes a reaction towards acetyl CoA. These recombinant yeast cells may be used in processes for the production of itaconic acid, 4-methyl itaconate, 1-methyl itaconate or 1,4-dimethyl itaconate.
• PROCESS FOR PRODUCING ITACONIC ACID AND ITACONIC ACID ESTERS UNDER ANAEROBIC CONDITIONS
  申请人：DSM IP Assets B.V.

  公开号：US20180245106A1

  公开（公告）日：2018-08-30

  The present invention relates to a method for the production of itaconic acid or an ester of itaconic acid, which method comprises fermenting a recombinant cell capable of producing itaconic acid or an ester of itaconic acid in a suitable fermentation medium under anaerobic conditions, wherein the itaconic acid or ester of itaconic acid is produced.