The present invention relates generally to a genetic sequence encoding a polypeptide having flavonoid 3', 5'-hydroxylase (F3'5'H) activity and to the use of the genetic sequence and/or its corresponding polypeptide thereof inter alia to manipulate color in flowers or parts thereof or in other plant tissue. More particularly, the F3'5'H has the ability to modulate dihydrokaempferol (DHK) metabolism as well as the metabolism of other substrates such as dihydroquercetin (DHQ), naringenin and eriodictyol. Even more particularly, the present invention provides a genetic sequence encoding a polypeptide having F3'5'H activity when expressed in rose or gerbera or botanically related plants. The instant invention further relates to antisense and sense molecules or RNAi-inducing molecules corresponding to all or part of the subject genetic sequence or a transcript thereof. The present invention further relates to promoters which operate efficiently in plants such as rose, gerbera or botanically related plants.
本发明一般涉及编码具有类
黄酮 3',5'-羟化酶(F3'5'H)活性的
多肽的
基因序列,以及使用该
基因序列和/或其相应的
多肽来调节花朵或其部分或其他植物组织的颜色。更特别的是,F3'5'H 具有调节二氢
山奈酚(DHK)代谢以及其他底物如二氢
槲皮素(DHQ)、
柚皮苷和麦角
酪醇代谢的能力。更具体地说,本发明提供了一种
基因序列,该序列编码的
多肽在玫瑰或非洲菊或植物学上相关的植物中表达时具有 F3'5'H 活性。本发明还涉及与全部或部分主题
基因序列或其转录本相对应的反义和有义分子或 RNAi 诱导分子。本发明进一步涉及在植物如蔷薇、非洲菊或植物学相关植物中有效运行的启动子。