2-((S)-2-(((benzyloxy)carbonyl)amino)-3-oxo-3-(2-oxo-2-phenylethoxy)propyl) 1-(tert-butyl) (S)-pyrrolidine-1,2-dicarboxylate | 502964-81-0

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 英文名称: 2-((S)-2-(((benzyloxy)carbonyl)amino)-3-oxo-3-(2-oxo-2-phenylethoxy)propyl) 1-(tert-butyl) (S)-pyrrolidine-1,2-dicarboxylate
• 英文别名: 2-[(S)-2-Benzyloxycarbonylamino-2-(2-oxo-2-phenyl-ethoxycarbonyl)-ethyl] 1-tert-butyl (S)-pyrrolidine-1,2-dicarboxylate；1-O-tert-butyl 2-O-[(2S)-3-oxo-3-phenacyloxy-2-(phenylmethoxycarbonylamino)propyl] (2S)-pyrrolidine-1,2-dicarboxylate
• CAS: 502964-81-0
• 化学式: C₂₉H₃₄N₂O₉
• 分子量: 554.597
• InChiKey: DGGMTJBKQOYTCI-GOTSBHOMSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  4.1
• 重原子数：
  40
• 可旋转键数：
  15
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.41
• 拓扑面积：
  138
• 氢给体数：
  1
• 氢受体数：
  9

反应信息

• 作为反应物：
  描述：

  2-((S)-2-(((benzyloxy)carbonyl)amino)-3-oxo-3-(2-oxo-2-phenylethoxy)propyl) 1-(tert-butyl) (S)-pyrrolidine-1,2-dicarboxylate 在 N,N-二异丙基乙胺 、 N-[(dimethylamino)-3-oxo-1H-1,2,3-triazolo[4,5-b]pyridin-1-yl-methylene]-N-methylmethanaminium hexafluorophosphate 作用下， 以 二氯甲烷 、 N,N-二甲基甲酰胺 为溶剂， 反应 1.0h， 生成 tert-butyl(S)-2-(((S)-1-(((S)-1-((S)-2-(((S)-2-(((benzyloxy)carbonyl)amino)-3-oxo-3-(2-oxo-2-phenylethoxy)propoxy)carbonyl)pyrrolidin-1-yl)-1-oxopropan-2-yl)amino)-1-oxopropan-2-yl)(methyl)carbamoyl)pyrrolidine-1-carboxylate:
  参考文献：
  名称：

  Restriction of the Conformational Dynamics of the Cyclic Acyldepsipeptide Antibiotics Improves Their Antibacterial Activity

  摘要：

  The cyclic acyldepsipeptide (ADEP) antibiotics are a new class of antibacterial agents that kill bacteria via a mechanism that is distinct from all clinically used drugs. These molecules bind and dysregulate the activity of the ClpP peptidase. The potential of these antibiotics as antibacterial drugs has been enhanced by the elimination of pharmacological liabilities through medicinal chemistry efforts. Here, we demonstrate that the ADEP conformation observed in the ADEP-ClpP crystal structure is fortified by transannular hydrogen bonding and can be further stabilized by judicious replacement of constituent amino acids within the peptidolactone core structure with more conformationally constrained counterparts. Evidence supporting constraint of the molecule into the bioactive conformer was obtained by measurements of deuterium-exchange kinetics of hydrogens that were proposed to be engaged in transannular hydrogen bonds. We show that the rigidified ADEP analogs bind and activate ClpP at lower concentrations in vitro. Remarkably, these compounds have up to 1200-fold enhanced antibacterial activity when compared to those with the peptidolactone core structure common to two ADEP natural products. This study compellingly demonstrates how rational modulation of conformational dynamics may be used to improve the bioactivities of natural products.

  DOI：

  10.1021/ja410385c
• 作为产物：
  描述：

  N-苄氧羰基-L-丝氨酸 在 4-二甲氨基吡啶 、 盐酸-N-乙基-Nˊ-(3-二甲氨基丙基)碳二亚胺 、 三乙胺 作用下， 以 二氯甲烷 、 乙酸乙酯 为溶剂， 反应 3.0h， 生成 2-((S)-2-(((benzyloxy)carbonyl)amino)-3-oxo-3-(2-oxo-2-phenylethoxy)propyl) 1-(tert-butyl) (S)-pyrrolidine-1,2-dicarboxylate
  参考文献：
  名称：

  Restriction of the Conformational Dynamics of the Cyclic Acyldepsipeptide Antibiotics Improves Their Antibacterial Activity

  摘要：

  The cyclic acyldepsipeptide (ADEP) antibiotics are a new class of antibacterial agents that kill bacteria via a mechanism that is distinct from all clinically used drugs. These molecules bind and dysregulate the activity of the ClpP peptidase. The potential of these antibiotics as antibacterial drugs has been enhanced by the elimination of pharmacological liabilities through medicinal chemistry efforts. Here, we demonstrate that the ADEP conformation observed in the ADEP-ClpP crystal structure is fortified by transannular hydrogen bonding and can be further stabilized by judicious replacement of constituent amino acids within the peptidolactone core structure with more conformationally constrained counterparts. Evidence supporting constraint of the molecule into the bioactive conformer was obtained by measurements of deuterium-exchange kinetics of hydrogens that were proposed to be engaged in transannular hydrogen bonds. We show that the rigidified ADEP analogs bind and activate ClpP at lower concentrations in vitro. Remarkably, these compounds have up to 1200-fold enhanced antibacterial activity when compared to those with the peptidolactone core structure common to two ADEP natural products. This study compellingly demonstrates how rational modulation of conformational dynamics may be used to improve the bioactivities of natural products.

  DOI：

  10.1021/ja410385c

文献信息

• Antibacterial macrocycles
  申请人：Hinzen Berthold

  公开号：US20050107288A1

  公开（公告）日：2005-05-19

  The present invention relates to compounds of the general formula (I), processes for their preparation, pharmaceutical compositions comprising them, and their use in the treatment of diseases in humans or animals.

  本发明涉及一般式（I）的化合物，其制备方法，包含它们的制药组合物以及它们在治疗人类或动物疾病方面的用途。
• ANTIBAKTERIELLE MAKROZYKLEN
  申请人：Bayer HealthCare AG

  公开号：EP1430075B1

  公开（公告）日：2007-01-03
• US7405201B2
  申请人：——

  公开号：US7405201B2

  公开（公告）日：2008-07-29
• Restriction of the Conformational Dynamics of the Cyclic Acyldepsipeptide Antibiotics Improves Their Antibacterial Activity
  作者：Daniel W. Carney、Karl R. Schmitz、Jonathan V. Truong、Robert T. Sauer、Jason K. Sello

  DOI：10.1021/ja410385c

  日期：2014.2.5

  The cyclic acyldepsipeptide (ADEP) antibiotics are a new class of antibacterial agents that kill bacteria via a mechanism that is distinct from all clinically used drugs. These molecules bind and dysregulate the activity of the ClpP peptidase. The potential of these antibiotics as antibacterial drugs has been enhanced by the elimination of pharmacological liabilities through medicinal chemistry efforts. Here, we demonstrate that the ADEP conformation observed in the ADEP-ClpP crystal structure is fortified by transannular hydrogen bonding and can be further stabilized by judicious replacement of constituent amino acids within the peptidolactone core structure with more conformationally constrained counterparts. Evidence supporting constraint of the molecule into the bioactive conformer was obtained by measurements of deuterium-exchange kinetics of hydrogens that were proposed to be engaged in transannular hydrogen bonds. We show that the rigidified ADEP analogs bind and activate ClpP at lower concentrations in vitro. Remarkably, these compounds have up to 1200-fold enhanced antibacterial activity when compared to those with the peptidolactone core structure common to two ADEP natural products. This study compellingly demonstrates how rational modulation of conformational dynamics may be used to improve the bioactivities of natural products.