3-磷酸甘油脱氢酶 | 9075-65-4

• 物质功能分类: 有机原料 - 羧酸类化合物及衍生物
• 分子结构分类: 氧化还原酶 - 作用于CH-OH供体组 - 以NAD+或NADP+为受体
• 中文名称: 3-磷酸甘油脱氢酶
• 中文别名: Α-甘油磷酸脱氢酶；α-甘油磷酸脱氢酶来源于兔肌肉；SN-甘油-3-磷酸:NAD+2-氧化还原酶；甘油3-磷酸脱氢酶(兔肌肉)；SN-甘油-3-磷酸脱氢酶来源于兔肌肉
• 英文名称: glycerol-3-phosphate dehydrogenase (NAD+)
• 英文别名: alpha-glycerol phosphate dehydrogenase (NAD+)；alpha-glycerophosphate dehydrogenase (NAD+)；glycerol 1-phosphate dehydrogenase；glycerol phosphate dehydrogenase (NAD+)；glycerophosphate dehydrogenase (NAD+)；hydroglycerophosphate dehydrogenase；L-alpha-glycerol phosphate dehydrogenase；L-alpha-glycerophosphate dehydrogenase；L-glycerol phosphate dehydrogenase；L-glycerophosphate dehydrogenase (ambiguous)；NAD+-alpha-glycerophosphate dehydrogenase；NAD+-dependent glycerol phosphate dehydrogenase；NAD+-dependent glycerol-3-phosphate dehydrogenase；NAD+-L-glycerol-3-phosphate dehydrogenase；NAD+-linked glycerol 3-phosphate dehydrogenase；NADH-dihydroxyacetone phosphate reductase；L-glycerol-3-phosphate dehydrogenase (ambiguous)；glycerophosphate dehydrogenase
• CAS: 9075-65-4

物化性质

• 稳定性/保质期：
  Analysis Note: Protein determined by biuret. Unit Definition: One unit will convert 1.0 μmole of dihydroxyacetone phosphate to α-glycerophosphate per minute at pH 7.4 and 25 °C. Physical Form: Lyophilized sulfate-free powder containing buffer salts as citrate and EDTA.

反应信息

• 作为试剂：
  描述：

  1,3-dihydroxyacetone phosphate 在 磷酸丙糖异构酶 、 3-磷酸甘油脱氢酶 、 还原型辅酶Ⅰ 作用下， 以 aq. buffer 为溶剂， 反应 0.17h， 生成 α-glycerophosphate
  参考文献：
  名称：

  l-Rhamnulose-1-phosphate and l-fuculose-1-phosphate aldolase mediated multi-enzyme cascade systems for nitrocyclitol synthesis

  摘要：

  One-pot multistep stereoselective cascade reactions were implemented for the straightforward synthesis of various nitrocyclitols. Two kinases, an aldolase and a phosphatase were involved in this process, together with a spontaneous intramolecular Henry reaction to provide the nitrocyclitol moiety. The C-C bond formation catalysed by the aldolase and the nitroaldol reactions were key steps to build the carbocycle stereoselectively. The aldolase acceptor substrates were all 4-nitrobutanal structurally based, either hydroxylated or unsubstituted at the C2 and/or C3 positions. L-Fuculose-1-phosphate aldolase (FucA) catalysed the formation of the expected (R,R)- or D-erythro aldol, except in the case of 4-nitrobutanal, from which the epimeric (R,S)- or L-threo aldol was also formed. L-Rhamnulose-1-phosphate aldolase consistently formed the expected (R,S)- or L-threo aldol together with a minor amount of (R,R)- or D-elythro aldol. The intramolecular Henry reaction was also found to be stereoselective, occurring spontaneously once the aldol was formed due to the presence of both ketone and a terminally positioned nitro group. The combination of this set of reactions successfully furnished 11 nitrocyclitols which have not been described previously in the literature. (C) 2014 Elsevier B.V. All rights reserved.

  DOI：

  10.1016/j.molcatb.2014.10.016