6-[amino(propyl)amino]-3-methyl-1H-pyrimidine-2,4-dione | 1186491-90-6

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 二嗪类
• 英文名称: 6-[amino(propyl)amino]-3-methyl-1H-pyrimidine-2,4-dione
• CAS: 1186491-90-6
• 化学式: C₈H₁₄N₄O₂
• 分子量: 198.225
• InChiKey: LBSBNUMVEBWHLU-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -0.3
• 重原子数：
  14
• 可旋转键数：
  3
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.5
• 拓扑面积：
  78.7
• 氢给体数：
  2
• 氢受体数：
  4

反应信息

• 作为反应物：
  描述：

  6-[amino(propyl)amino]-3-methyl-1H-pyrimidine-2,4-dione 在 溶剂黄146 、 sodium nitrite 作用下， 以 乙醇 、 水 为溶剂， 反应 21.0h， 生成 3-(4-ethylphenyl)-6-methyl-1-propylpyrimido[5,4-e][1,2,4]triazine-5,7-dione
  参考文献：
  名称：

  Toxoflavins and Deazaflavins as the First Reported Selective Small Molecule Inhibitors of Tyrosyl-DNA Phosphodiesterase II

  摘要：

  The recently discovered enzyme tyrosyl-DNA phosphodiesterase 2 (TDP2) has been implicated in the topoisomerase-mediated repair of DNA damage. In the clinical setting, it has been hypothesized that TDP2 may mediate drug resistance to topoisomerase II (topo II) inhibition by etoposide. Therefore, selective pharmacological inhibition of TDP2 is proposed as a novel approach to overcome intrinsic or acquired resistance to topo II-targeted drug therapy. Following a high-throughput screening (HTS) campaign, toxoflavins and deazaflavins were identified as the first reported sub-micromolar and selective inhibitors of this enzyme. Toxoflavin derivatives appeared to exhibit a clear structure-activity relationship (SAP.) for TDP2 enzymatic inhibition. However, we observed a key redox liability of this series, and this, alongside early in vitro drug metabolism and pharmacokinetics (DMPK) issues, precluded further exploration. The deazaflavins were developed from a singleton HTS hit. This series showed distinct SAR and did not display redox activity; however low cell permeability proved to be a challenge.

  DOI：

  10.1021/jm400568p
• 作为产物：
  描述：

  草酸丙基肼盐 、 6-氯-3-甲基尿嘧啶 在 三乙胺 作用下， 以 乙醇 为溶剂， 反应 16.0h， 生成 6-[amino(propyl)amino]-3-methyl-1H-pyrimidine-2,4-dione
  参考文献：
  名称：

  Toxoflavins and Deazaflavins as the First Reported Selective Small Molecule Inhibitors of Tyrosyl-DNA Phosphodiesterase II

  摘要：

  The recently discovered enzyme tyrosyl-DNA phosphodiesterase 2 (TDP2) has been implicated in the topoisomerase-mediated repair of DNA damage. In the clinical setting, it has been hypothesized that TDP2 may mediate drug resistance to topoisomerase II (topo II) inhibition by etoposide. Therefore, selective pharmacological inhibition of TDP2 is proposed as a novel approach to overcome intrinsic or acquired resistance to topo II-targeted drug therapy. Following a high-throughput screening (HTS) campaign, toxoflavins and deazaflavins were identified as the first reported sub-micromolar and selective inhibitors of this enzyme. Toxoflavin derivatives appeared to exhibit a clear structure-activity relationship (SAP.) for TDP2 enzymatic inhibition. However, we observed a key redox liability of this series, and this, alongside early in vitro drug metabolism and pharmacokinetics (DMPK) issues, precluded further exploration. The deazaflavins were developed from a singleton HTS hit. This series showed distinct SAR and did not display redox activity; however low cell permeability proved to be a challenge.

  DOI：

  10.1021/jm400568p

文献信息

• Pyrimido[5,4-e][1,2,4]triazine-5,7(1H,6H)-dione derivatives as novel small molecule chaperone amplifiers
  作者：Yuefen Zhou、Linyi Wei、Thomas P. Brady、P.S. Murali Mohan Redddy、Tram Nguyen、Jinhua Chen、Qingyan Au、Il Sang Yoon、Gary Yip、Bin Zhang、Jack R. Barber、Shi Chung Ng

  DOI：10.1016/j.bmcl.2009.05.073

  日期：2009.8

  Pyrimido[5,4-e][1,2,4]triazine-5,7(1H,6H)-dione derivatives were investigated as novel small molecule amplifiers of heat shock factor 1 transcriptional activity. Lead optimization led to the discovery of compound 4A-13, which displayed potent HSF1 activity under mild heat stress (EC50 = 2.5 μM) and significant cytoprotection in both rotenone (EC50 = 0.23 μM) and oxygen-glucose deprivation cell toxicity

  嘧啶[5,4- e ] [1,2,4]三嗪-5,7（1 H，6 H）-二酮衍生物被研究为热休克因子1转录活性的新型小分子放大器。铅的优化导致化合物4A-13的发现，该化合物在轻度热应激下（EC 50  = 2.5μM）表现出强大的HSF1活性，在鱼藤酮（EC 50  = 0.23μM）和氧-葡萄糖剥夺细胞毒性模型中均具有显着的细胞保护作用（在2.5μM时具有80％的保护）。