(2-iodoacetyl)-L-tyrosine | 150956-51-7

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 英文名称: (2-iodoacetyl)-L-tyrosine
• 英文别名: N-iodoacetyl-L-tyrosine；N-Jodacetyl-L-tyrosin；(2S)-3-(4-hydroxyphenyl)-2-[(2-iodoacetyl)amino]propanoic acid
• CAS: 150956-51-7
• 化学式: C₁₁H₁₂INO₄
• 分子量: 349.125
• InChiKey: WQTOCBBVLGMXOH-VIFPVBQESA-N

物化性质

• 熔点：
  166-167 °C
• 沸点：
  582.4±50.0 °C(Predicted)
• 密度：
  1.837±0.06 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  0.5
• 重原子数：
  17
• 可旋转键数：
  5
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.27
• 拓扑面积：
  86.6
• 氢给体数：
  3
• 氢受体数：
  4

反应信息

• 作为产物：
  描述：

  氯化碘乙酸 、 L-酪氨酸乙酯 在 氯仿 作用下， 生成 (2-iodoacetyl)-L-tyrosine
  参考文献：
  名称：

  Abderhalden; Abderhalden, Fermentforschung, 1942, vol. 16, p. 48,56

  摘要：

  DOI：

文献信息

• Labelling of peptides and proteins
  申请人：Nordheim, Alfred, Prof. Dr.

  公开号：EP1059531A1

  公开（公告）日：2000-12-13

  This invention relates to methods for labelling peptides and/or proteins in preparative and analytical electrophoresis, especially as sample preparation for isoelectric focussing, wherein side chains of specific amino acid residues of the peptides and/or proteins are derivatized and labelled. The purpose of the invention is to control the isoelectric point of the peptides and/or proteins to permit isoelectric focussing of the samples with reliable results. In one embodiment of the invention cysteine residues of the peptides and/or proteins are alkylated, such that the modified amino acid side chain can be labelled, e.g. iodinated, along with tyrosine during standard conditions for tyrosine labelling. The invention permits unincorporated label to be electrophoretically removed from the sample during subsequent electrophoresis, thus dramatically improving the ratio of specific protein-bound signal to non-specific background signal upon isotopic detection.

  本发明涉及在制备和分析电泳中对肽和/或蛋白质进行标记的方法，特别是作为等电点聚焦的样品制备，其中对肽和/或蛋白质的特定氨基酸残基的侧链进行衍生化和标记。本发明的目的是控制肽和/或蛋白质的等电点，以便对样品进行等电聚焦，并获得可靠的结果。在本发明的一个实施方案中，肽和/或蛋白质的半胱氨酸残基被烷基化，这样，在酪氨酸标记的标准条件下，经修饰的氨基酸侧链可与酪氨酸一起被标记，如碘化。本发明允许在随后的电泳过程中将未结合的标签从样品中电泳去除，从而大大提高了同位素检测时特异性蛋白质结合信号与非特异性背景信号的比率。
• [EN] LABELLING OF PEPTIDES AND PROTEINS<br/>[FR] MARQUAGE DE PEPTIDES ET DE PROTEINES
  申请人：NORDHEIM ALFRED

  公开号：WO2000077520A1

  公开（公告）日：2000-12-21

  This invention relates to methods for labelling peptides and/or proteins in preparative and analytical electrophoresis, especially as sample preparation for isoelectric focussing, wherein side chains of specific amino acid residues of the peptides and/or proteins are derivatized and labelled. The purpose of the invention is to control the isoelectric point of the peptides and/or proteins to permit isoelectric focussing of the samples with reliable results. In one embodiment of the invention cysteine residues of the peptides and/or proteins are alkylated, such that the modified amino acid side chain can be labelled, e.g. iodinated, along with tyrosine during standard conditions for tyrosine labelling. The invention permits unincorporated label to be electrophoretically removed from the sample during subsequent electrophoresis, thus dramatically improving the ratio of specific protein-bound signal to non-specific background signal upon isotopic detection.
• [EN] IMPROVED PROTEIN ANALYSIS<br/>[FR] ANALYSE AMELIOREE DES PROTEINES
  申请人：PROTEOSYS AG

  公开号：WO2001094945A2

  公开（公告）日：2001-12-13

  A method for improving the solubility and/or hydrophilicity of peptides or proteins in solvents is characterized in that one or more alkylcomponents is introduced to the peptides or proteins by reacting them with an alkylating reagent. This alkylating reagent is comprising at least one polar and/or ionisable groups and preferably at least one functional group.