p-<(α-methyl-6-nitropiperonyloxycarbonyl)oxy>nitrobenzene | 865872-19-1

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 苯并间二氧杂环戊烯类
• 英文名称: p-<(α-methyl-6-nitropiperonyloxycarbonyl)oxy>nitrobenzene
• CAS: 865872-19-1
• 化学式: C₁₆H₁₂N₂O₉
• 分子量: 376.279
• InChiKey: VAPIPLULRUQQRI-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  3.51
• 重原子数：
  27.0
• 可旋转键数：
  5.0
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.19
• 拓扑面积：
  140.27
• 氢给体数：
  0.0
• 氢受体数：
  9.0

反应信息

• 作为反应物：
  描述：

  p-<(α-methyl-6-nitropiperonyloxycarbonyl)oxy>nitrobenzene 在 盐酸 、 sodium hydride 作用下， 以 四氢呋喃 、 氯仿 、 N,N-二甲基甲酰胺 为溶剂， 反应 24.5h， 生成 N-1',N-3'-bis(α-methyl-6-nitropiperonyloxycarbonyl)biotin
  参考文献：
  名称：

  Spatially-Addressable Immobilization of Macromolecules on Solid Supports

  摘要：

  A method is described for immobilization of receptors, antibodies, or other macromolecules at precise locations on solid substrates. We have combined photolithographic techniques with the use of a ''caged'' biotin analogue that has been covalently linked to the substrate surface. Exposure to near UV light through a photolithographic mask yields biotin sites for streptavidin binding. Biotinylated macromolecules are then immobilized via a biotin-streptavidin-biotin bridge. Molecules may be attached at selected locations by carrying out repeated rounds of exposure, streptavidin binding, and application of the biotinylated reagent. We have demonstrated the immobilization of fluorescein-streptavidin molecules in 500 mu m x 500 mu m sites, and the localization of two biotinylated antibodies at different sites on a planar substrate surface. We anticipate that the technique will prove useful in drug screening, diagnostics, and biosensor applications.

  DOI：

  10.1021/ja00154a003
• 作为产物：
  描述：

  对硝基苯基氯甲酸酯 、 1-(4,5-亚甲基二氧基-2-硝基苯酚)-2-乙醇 在 吡啶 作用下， 以 二氯甲烷 为溶剂， 反应 64.0h， 以93%的产率得到p-<(α-methyl-6-nitropiperonyloxycarbonyl)oxy>nitrobenzene
  参考文献：
  名称：

  Spatially-Addressable Immobilization of Macromolecules on Solid Supports

  摘要：

  A method is described for immobilization of receptors, antibodies, or other macromolecules at precise locations on solid substrates. We have combined photolithographic techniques with the use of a ''caged'' biotin analogue that has been covalently linked to the substrate surface. Exposure to near UV light through a photolithographic mask yields biotin sites for streptavidin binding. Biotinylated macromolecules are then immobilized via a biotin-streptavidin-biotin bridge. Molecules may be attached at selected locations by carrying out repeated rounds of exposure, streptavidin binding, and application of the biotinylated reagent. We have demonstrated the immobilization of fluorescein-streptavidin molecules in 500 mu m x 500 mu m sites, and the localization of two biotinylated antibodies at different sites on a planar substrate surface. We anticipate that the technique will prove useful in drug screening, diagnostics, and biosensor applications.

  DOI：

  10.1021/ja00154a003