盐酸-NA-苯甲酰-1-精氨酸酰-4-硝基苯胺

• 分子结构分类: 有机化合物 - 苯类化合物 - 苯和取代衍生物
• 中文名称: 盐酸-NA-苯甲酰-1-精氨酸酰-4-硝基苯胺
• 中文别名: NA-苯甲酰-L-精氨酸酰-4-硝基苯胺盐酸盐；NA-苯甲酰-L-精氨酸P-硝基苯胺盐酸盐；N-α-苯甲酰基-L-精氨酸对硝基苯胺盐酸盐
• 英文名称: N-α-benzoyl-L-arginine 4-nitroanilide hydrochloride
• 英文别名: Nα-benzoyl-L-arginine-4-nitroanilide hydrochloride；Nα-benzoyl-L-arginine-p-nitroanilide hydrochloride；N-benzoyl-L-arginine-p-nitroanilide hydrochloride；N-benzoyl-Arg 4-nitroanilide hydrochloride；Bz-L-Arg-p-NA hydrochloride；L-BAPNA
• 化学式: C₁₉H₂₂N₆O₄*ClH
• 分子量: 434.882
• InChiKey: DEOKFPFLXFNAON-NTISSMGPSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  1.81
• 重原子数：
  30
• 可旋转键数：
  8
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.21
• 拓扑面积：
  168
• 氢给体数：
  5
• 氢受体数：
  5

反应信息

• 作为反应物：
  描述：

  盐酸-NA-苯甲酰-1-精氨酸酰-4-硝基苯胺 在 trypsin from bovine pancreas 作用下， 以 N,N-二甲基甲酰胺 为溶剂， 生成 N-ALPHA-苄酰-L-精氨酸
  参考文献：
  名称：

  Podzemna, V.; Slovakova, M.; Kourkova, L., Journal of Thermal Analysis and Calorimetry, 2010, vol. 101, p. 715 - 719

  摘要：

  DOI：

文献信息

• Development of Sulfhydryl-Reactive Silica for Protein Immobilization in High-Performance Affinity Chromatography
  作者：Rangan Mallik、Chunling Wa、David S. Hage

  DOI：10.1021/ac061779j

  日期：2007.2.1

  Two techniques were developed for the immobilization of proteins and other ligands to silica through sulfhydryl groups. These methods made use of maleimide-activated silica (the SMCC method) or iodoacetyl-activated silica (the SIA method). The resulting supports were tested for use in high-performance affinity chromatography by employing human serum albumin (HSA) as a model protein. Studies with normal and iodoacetamide-modified HSA indicated that these methods had a high selectivity for sulfhydryl groups on this protein, which accounted for the coupling of 77−81% of this protein to maleimide- or iodoacetyl-activated silica. These supports were also evaluated in terms of their total protein content, binding capacity, specific activity, nonspecific binding, stability, and chiral selectivity for several test solutes. HSA columns prepared using maleimide-activated silica gave the best overall results for these properties when compared to HSA that had been immobilized to silica through the Schiff base method (i.e., an amine-based coupling technique). A key advantage of the supports developed in this work is that they offer the potential of giving greater site-selective immobilization and ligand activity than amine-based coupling methods. These features make these supports attractive in the development of protein columns for such applications as the study of biological interactions and chiral separations.

  研究人员开发了两种通过巯基将蛋白质和其他配体固定在二氧化硅上的技术。这两种方法分别利用马来酰亚胺活化二氧化硅（SMCC法）和碘乙酰活化二氧化硅（SIA法）。研究人员用人血清白蛋白（HSA）作为模型蛋白，测试了所得载体在高效亲和色谱法中的使用效果。对正常和碘乙酰胺修饰HSA的研究表明，这两种方法对蛋白质上的巯基具有高度选择性，可将77-81%的蛋白质与马来酰亚胺或碘乙酰活化二氧化硅偶联。研究人员还评估了这些载体的总蛋白含量、结合能力、特异性活性、非特异性结合、稳定性以及对几种测试溶质的立体选择性。与通过席夫碱法（一种基于胺的偶联技术）固定在二氧化硅上的HSA相比，用马来酰亚胺活化二氧化硅制备的HSA柱在这些特性上具有最佳的整体效果。这项工作中开发的载体的一个关键优势在于，它们具有比基于胺的偶联方法更高的位点选择性固定和配体活性。这些特性使这些载体在开发蛋白质色谱柱方面具有吸引力，可用于生物相互作用和手性分离等应用。
• Express sequence tag analysis – Identification of anseriformes trypsin genes from full-length cDNA library of the duck (Anas platyrhynchos) and characterization of their structure and function
  作者：Haining Yu、Shasha Cai、Jiuxiang Gao、Chen Wang、Xue Qiao、Hui Wang、Lan Feng、Yipeng Wang

  DOI：10.1134/s0006297916020097

  日期：2016.2

  measured. The three dimensional structures of trypsin-IAP and trypsin-IIAP were predicted by homology modeling, and the conserved residues required for functionality were identified. Two loops controlling the specificity of the trypsin and the substrate-binding pocket represented in the model are almost identical in primary sequences and backbone tertiary structures of the trypsin families.

  胰蛋白酶是通过破坏赖氨酸和精氨酸残基羧基侧的肽键，在动物蛋白消化中很重要的关键蛋白，因此已广泛用于各种生物技术过程中。在当前的研究中，从鸭（Anas platyrhynchos）中构建了一个容量为5·10（5）CFU / ml的全长cDNA文库。使用表达序列标签（EST）测序，鉴定出编码两个胰蛋白酶的基因，然后通过cDNA末端快速扩增（RACE）-PCR获得两个全长的胰蛋白酶cDNA。使用Blast，它们被分类为胰蛋白酶I和II子家族，但是都编码位于C端的信号肽，激活肽和223-aa成熟蛋白。推导的两个成熟蛋白分别命名为胰蛋白酶-IAP和胰蛋白酶-IIAP，其理论等电点（pI）和分子量（MW）分别为7.99 / 23466.4 Da和4.65 / 24066.0 Da。基因的分子表征通过详细的生物信息学分析进一步进行。系统发育分析表明，胰蛋白酶-IIAP具有不同于胰蛋白酶-IAP的进化模式
• Monoclonal antibodies recognizing human PSTI, hybridomas producing the same, and their preparation and use
  申请人：SHIONOGI & CO., LTD.

  公开号：EP0332421A2

  公开（公告）日：1989-09-13

  Monoclonal antibodies which recognize human PSTI and hybridomas producing the same are provided by the invention. A method for the determination of human PSTI using the monoclonal antibody is also provided.

  本发明提供了识别人类 PSTI 的单克隆抗体和产生这种抗体的杂交瘤。本发明还提供了一种使用单克隆抗体测定人类 PSTI 的方法。
• COMPOSITION OF INTER-ALPHA INHIBITOR PROTEINS FROM HUMAN PLASMA FOR THERAPEUTIC USE
  申请人：Prothera Biologics

  公开号：EP3087991A1

  公开（公告）日：2016-11-02

  This invention relates to Inter-alpha inhibitor proteins (lαlp). The invention further relates to processes for purification of lαlp compositions and their use for treatment of human diseases such as sepsis and septic shock, rheumatoid arthritis, cancer and infectious diseases.

  本发明涉及α间抑制蛋白（lαlp）。本发明还涉及 lαlp 组合物的纯化过程及其在治疗败血症和脓毒性休克、类风湿性关节炎、癌症和传染性疾病等人类疾病中的应用。
• Preparation and composition of inter-alpha inhibitor proteins from blood
  申请人：ProThera Biologics, Inc.

  公开号：US10076559B2

  公开（公告）日：2018-09-18

  The present invention generally provides processes for purification of Inter-alpha inhibitor proteins (IαIp) and compositions thereof from blood.

  本发明一般提供了从血液中纯化α间抑制蛋白（IαIp）及其组合物的工艺。