Provided are engineered polypeptides that are useful for the asymmetric synthesis of β-hydroxy-α-amino acids under industrial-relevant conditions. The engineered polypeptides disclosed are developed through directed evolution based on the ability of catalytic synthesis of (2S, 3R) -2-amino-3-hydroxy-3- (4-nitrophenyl) propanoic acid. Also provided are polynucleotides encoding the engineered polypeptides, host cells capable of expressing engineered polypeptides, and methods of producing β-hydroxy-α-amino acids using engineered polypeptides. Compared to other processes of preparation, the use of the engineered polypeptides for the preparation of β-hydroxy-α-amino acids results in high purity of the desired stereoisomers, mild reaction conditions, low pollution and low energy consumption. It has good industrial application prospects.
提供了经过工程改造的
多肽,可用于在工业相关条件下不对称合成β-羟基-
α-氨基酸。所述经过改造的
多肽是通过基于催化合成(2S,3R)-2-
氨基-3-羟基-3-(4-
硝基苯基)
丙酸的能力进行定向进化而开发的。还提供了编码经过改造的
多肽的多核苷酸、能够表达经过改造的
多肽的宿主细胞,以及使用经过改造的
多肽生产β-羟基-
α-氨基酸的方法。与其他制备过程相比,使用经过改造的
多肽制备β-羟基-
α-氨基酸可得到所需立体异构体的高纯度,反应条件温和,污染和能耗低。具有良好的工业应用前景。