Deoxynucleoside triphosphates bearing histamine, carboxylic acid, and hydroxyl residues – synthesis and biochemical characterization
作者:Marcel Hollenstein
DOI:10.1039/c3ob40842f
日期:——
Modified nucleosidetriphosphates (dAHsTP, dUPOHTP, and dCValTP) bearing imidazole, hydroxyl, and carboxylic acid residues connected to the purine and pyrimidine bases through alkyne linkers were prepared. These modified dN*TPs were excellent substrates for various DNA polymerases in primerextensionreactions. Moreover, the combined use of terminal deoxynucleotidyl transferase (TdT) and the modified