A fluorescent indicator-displacement molecular imprinting sensor array based on phenylboronic acid functionalized mesoporous silica was developed for discriminating saccharides. d-Fructose imprinted material (FruIM), d-xylose imprinted material (XylIM) together with a control blank nonimprinted material (NIM) were synthesized as the elements of the imprinting sensor array. Spectrofluorimetric titrations of the three materials with eight selected saccharides were carried out, and Stern−Volmer quenching constants (KSV) of NIM, FruIM, and XylIM with the eight selected saccharides were obtained to investigate the interaction of the materials with saccharides. The present approach couples molecular imprinting technique to indicator-displacement strategy with the use of one conventional saccharide receptor (phenylboronic acid) and one commercially available fluorescent dye (Alizarin Red S., ARS) as the indicator, and allows identifying two template saccharides (d-fructose and d-xylose) plus eight nontemplate saccharides (d-arabinose, d-glucose, d-galactose, d-mannose, l-sorbose, d-ribose, l-rhamnose and sucrose). The principal component analysis (PCA) plot shows a clear discrimination of the 10 tested saccharides at 100 mM and the first principal component possesses 94.8% of the variation. Besides, the developed saccharide imprinted sensor array is successfully applied to discriminating three brands of orange juice beverage.
基于苯
硼酸功能化介孔
二氧化硅开发了一种荧光指示剂置换分子印迹传感器阵列,用于鉴别
糖类。 将d-
果糖印迹材料(FruIM)、d-
木糖印迹材料(XylIM)及对照空白非印迹材料(NIM)分别合成作为印迹传感器阵列的组成元素。 并对三种材料与八种糖进行了荧光光谱滴定,同时得到NIM、FruIM和XylIM与八种糖的Stern-Volmer淬灭常数(KSV),从而探究材料与糖的相互作用。 本研究通过将分子印迹技术与指示剂置换法相结合,同时采用一种传统的
糖类受体(苯
硼酸)和一种商业化的荧光
染料(
茜素红S, ARS)作为荧光指示剂,从而实现了对两种模板糖(d-
果糖和d-
木糖)和八种非模板糖(d-
阿拉伯糖、d-
葡萄糖、d-半
乳糖、d-
甘露糖、l-
山梨糖、d-
核糖、l-
鼠李糖和
蔗糖)的鉴别。 主成分1 分析(PCA)图清楚地描述了10 种(100mM)
糖类别,其中第一主成分占据了94.8% 的变化。此外,所开发的糖分子印迹传感器阵列成功地用于鉴别三种品牌的橙汁型饮料。
