葡糖硫苷酶(-20℃) | 9025-38-1

• 物质功能分类: 生物化工 - 酶及辅酶类
• 分子结构分类: 水解酶 - 糖基化酶 - 糖苷酶，即水解O-和S-糖基化合物的酶
• 中文名称: 葡糖硫苷酶(-20℃)
• 英文名称: thioglucosidase
• 英文别名: myrosinase；sinigrinase；sinigrase
• CAS: 9025-38-1

物化性质

• 稳定性/保质期：
  Biochem/physiol Actions Myrosinases are present in many bacteria, fungi, and edible plants, including those of the Brassicaceae (Cruciferae) family. This enzyme hydrolyzes the S-glucosidic bond of a glucosinolate substrate to form an unstable aglycone that rearranges with the loss of sulfate, primarily yielding isothiocyanates; however, thiocyanates and nitriles are also formed. Many of the isothiocyanate products from aliphatic and aromatic glucosinolates exhibit cancer chemopreventive properties. Unit Definition One unit will produce 1.0 μmole glucose per minute from sinigrin at pH 6.0 at 25 °C.

反应信息

• 作为试剂：
  描述：

  sinigrin 在 葡糖硫苷酶(-20℃) 作用下， 以 水 为溶剂， 反应 72.0h， 生成 天然芥菜籽油
  参考文献：
  名称：

  Effects of Intact Glucosinolates and Products Produced from Glucosinolates in Myrosinase-Catalyzed Hydrolysis on the Potato Cyst Nematode (Globodera rostochiensis Cv. Woll)

  摘要：

  The potato cyst nematode (Globodera rostochiensis cv. Woll) is responsible for large yield losses in the potato crop, and opportunities for reducing the attack of these plant nematode species are, therefore, important. This study has been devoted to the testing of the in vitro effects on the potato cyst nematode of eight glucosinolates [prop-2-enyl-, but-3-enyl-, (R)-4-methylsulfinylbut-3-enyl-, benzyl-, phenethyl-, 4-hydroxybenzyl-, (2S)-2-hydroxybut-3-enyl-, and (2R)-2-hydroxy-2-phenylethylglucosinolate] as well as the effects of the products of this myrosinase-catalyzed hydrolysis. The glucosinolates were used at three concentrations, 0.05, 0.3, and 1.0 mg/mL, in the presence or absence of the enzyme myrosinase. The effects of the compounds on the mortality were monitored every 8 h for a 72 h period. No effects were found for any of the intact glucosinolates, However, when active myrosinase was included with 1 mg/mL phenethylglucosinolate at pH 6.5, 100% mortality was observed within just 16 h. A similar effect was achieved at the same concentration of benzyl-and prop-2-enylglucosinolates in the myrosinase-containing solutions, although longer exposures were required (24 and 40 h, respectively). The main aglucone products released from the glucosinolates with pronounced effects on the nematodes were shown to be the corresponding isothiocyanates. The results suggest that mixtures of these specific glucosinolates and active myrosinase or autolysis of plant materials containing these enzymes and glucosinolates might be used to control the potato cyst nematode in the soil.

  DOI：

  10.1021/jf010470s