Z-Gly-Gly-Leu-pNA | 53046-98-3

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 英文名称: Z-Gly-Gly-Leu-pNA
• 英文别名: Cbz-Gly-Gly-Leu-P-nitroanilide；benzyl N-[2-[[2-[[(2S)-4-methyl-1-(4-nitroanilino)-1-oxopentan-2-yl]amino]-2-oxoethyl]amino]-2-oxoethyl]carbamate
• CAS: 53046-98-3
• 化学式: C₂₄H₂₉N₅O₇
• mdl: MFCD00038383
• 分子量: 499.524
• InChiKey: IHRYETONKBXGOF-FQEVSTJZSA-N

物化性质

• 密度：
  1.297

计算性质

• 辛醇/水分配系数(LogP)：
  2.6
• 重原子数：
  36
• 可旋转键数：
  12
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.333
• 拓扑面积：
  171
• 氢给体数：
  4
• 氢受体数：
  7

反应信息

• 作为反应物：
  描述：

  Z-Gly-Gly-Leu-pNA 在 subtisilin 作用下， 以 various solvent(s) 为溶剂， 生成 4-硝基苯胺
  参考文献：
  名称：

  Enhancement of Catalytic Activity of Chemically Modified Subtilisin Carlsberg in Benzene by Adjustment of Lyophilization Conditions

  摘要：

  冻干条件的改变不仅显著提高了不溶性天然枯草杆菌蛋白酶的活性，还提高了苯中可溶性酶的活性。通过可溶化（提高100倍）和调整冻干条件（提高10倍），N-乙酰-L-苯丙氨酸乙酯与苯中1-丙醇的酯交换反应的催化活性（kcat/Km）提高了1000倍以上。用聚乙二醇（PEG-Sub）对枯草杆菌蛋白酶进行了修饰，使其能够在有机溶剂中溶解。研究了冻干前水溶液中酶溶液的pH值、盐或底物类似物对天然枯草杆菌蛋白酶和PEG-Sub催化活性的影响。天然枯草杆菌蛋白酶和PEG-Sub的活性都取决于pH值。盐的存在提高了PEG-Sub和天然枯草杆菌蛋白酶的活性。底物类似物N-Ac-L-Phe的存在也提高了天然枯草杆菌蛋白酶和PEG-Sub的活性。这些结果表明，有机溶剂可溶化的枯草杆菌蛋白酶受冻干条件的影响。

  DOI：

  10.1246/bcsj.73.1277

文献信息

• Isolation and Some Properties of a Serine Protease from the Fruits of<i>Cudrania cochinchinensis</i>(Lour.) Kudo et Masam.
  作者：Tetsuya UCHIKOBA、Kazunari ARIMA、Masayuki SHIMADA、Hiroo YONEZAWA、Mokoto KANEDA

  DOI：10.1271/bbb.64.623

  日期：2000.1

  An endopeptidase (Cudrania protease) with a molecular mass of 76 kDa has been purified from the fruits of Cudrania cochinchinensis (Lour.) Kudo et Masam. The enzyme was stable between pH 6 and 10 at 30°C for 60 min. The enzyme activity was inhibited by diisopropyl fluorophosphate, chymostatin, and aprotinin, but not by EDTA or pepstatin. These results indicated that the enzyme was a serine protease.

  从 Cudrania cochinchinensis (Lour.) Kudo et Masam 的果实中纯化出了一种分子质量为 76 kDa 的内肽酶（Cudrania 蛋白酶）。在 30°C 条件下，该酶在 pH 值 6 和 10 之间稳定 60 分钟。该酶的活性受氟磷酸二异丙酯、糜蛋白酶和阿普罗汀的抑制，但不受乙二胺四乙酸乙二酯（EDTA）和胃蛋白酶的抑制。这些结果表明该酶是一种丝氨酸蛋白酶。
• SEX-PEPTIDES RESISTANT TO PROTEOLYTIC DEGRADATION AND THEIR USE IN BIOLOGICAL CONTROL OF INSECTS
  申请人：Yissum Research Development Company of the Hebrew University of Jerusalem Ltd

  公开号：EP2183277A1

  公开（公告）日：2010-05-12
• US8677934B2
  申请人：——

  公开号：US8677934B2

  公开（公告）日：2014-03-25
• [EN] SEX-PEPTIDES RESISTANT TO PROTEOLYTIC DEGRADATION AND THEIR USE IN BIOLOGICAL CONTROL OF INSECTS<br/>[FR] PEPTIDES SEXUELS RÉSISTANT À UNE DÉGRADATION PROTÉOLYTIQUE ET LEUR UTILISATION DANS LA LUTTE BIOLOGIQUE CONTRE LES INSECTES
  申请人：YISSUM RES DEV CO

  公开号：WO2009016627A1

  公开（公告）日：2009-02-05

  An isolated nucleic acid molecule comprising, or consisting of, a polynucleotide sequence encoding an oligopeptide capable of inducing post-mating depression of receptivity to mating in female insects, wherein said oligopeptide is resistant to proteolytic degradation, is provided for expression in sterile transgenic male insects useful in the control of insect populations.
• Enhancement of Catalytic Activity of Chemically Modified Subtilisin Carlsberg in Benzene by Adjustment of Lyophilization Conditions
  作者：Oh Hyeong Kwon、Yukio Imanishi、Yoshihiro Ito

  DOI：10.1246/bcsj.73.1277

  日期：2000.5

  Alteration of the lyophilization conditions significantly enhanced not only the activity of insoluble native subtilisin Carlsberg but also that of solubilized enzyme in benzene. The catalytic activity (kcat/Km) for the transesterification reaction of N-acetyl-L-phenylalanine ethyl ester with 1-propanol in benzene was enhanced more than 1000 times by the solubilization (100-fold enhancement) and the adjustment of lyophilization conditions (10-fold enhancement). Subtilisin Carlsberg was modified with polyethylene glycol (PEG-Sub) so that it could be solubilized in organic solvents. The effects of pH of the aqueous enzyme solution, of salts, or of a substrate analog in the aqueous solution before the lyophilization process, on the catalytic activity of native subtilisin and PEG-Sub were investigated. The activities of both the native subtilisin and PEG-Sub depended on the pH. The activities of both PEG-Sub and the native subtilisin were enhanced by the presence of salts. The presence of a substrate analog, N-Ac-L-Phe, also enhanced the activity of the native subtilisin and PEG-Sub. These results demonstrated that the organic solvent-solubilized subtilisin was affected by the lyophilization conditions.

  冻干条件的改变不仅显著提高了不溶性天然枯草杆菌蛋白酶的活性，还提高了苯中可溶性酶的活性。通过可溶化（提高100倍）和调整冻干条件（提高10倍），N-乙酰-L-苯丙氨酸乙酯与苯中1-丙醇的酯交换反应的催化活性（kcat/Km）提高了1000倍以上。用聚乙二醇（PEG-Sub）对枯草杆菌蛋白酶进行了修饰，使其能够在有机溶剂中溶解。研究了冻干前水溶液中酶溶液的pH值、盐或底物类似物对天然枯草杆菌蛋白酶和PEG-Sub催化活性的影响。天然枯草杆菌蛋白酶和PEG-Sub的活性都取决于pH值。盐的存在提高了PEG-Sub和天然枯草杆菌蛋白酶的活性。底物类似物N-Ac-L-Phe的存在也提高了天然枯草杆菌蛋白酶和PEG-Sub的活性。这些结果表明，有机溶剂可溶化的枯草杆菌蛋白酶受冻干条件的影响。