1-(6-chloro-3-pyridylmethyl)-N-aminoimidazolidin-2-ylideneamine

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 吡啶及其衍生物
• 英文名称: 1-(6-chloro-3-pyridylmethyl)-N-aminoimidazolidin-2-ylideneamine
• 英文别名: 1-[(6-chloro-3-pyridyl) methyl]-N-aminoimidazolidin-2-ylideneamine；[1-[(6-chloropyridin-3-yl)methyl]-4,5-dihydroimidazol-2-yl]hydrazine
• 化学式: C₉H₁₂ClN₅
• 分子量: 225.681
• InChiKey: BIKUWKXPZWJNCS-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -0.1
• 重原子数：
  15
• 可旋转键数：
  3
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.33
• 拓扑面积：
  66.5
• 氢给体数：
  2
• 氢受体数：
  3

反应信息

• 作为反应物：
  描述：

  丁二酸酐 、 1-(6-chloro-3-pyridylmethyl)-N-aminoimidazolidin-2-ylideneamine 在 吡啶 作用下， 反应 12.0h， 以70.6%的产率得到4-(1-[(6-chloropyridin-3-yl)methyl]imidazolidin-2-ylidenehydrazine)-4-oxobutanoic acid
  参考文献：
  名称：

  Development of an ELISA for the Detection of the Residues of the Insecticide Imidacloprid in Agricultural and Environmental Samples

  摘要：

  A competitive enzyme-linked immunosorbent assay (ELISA) for the chloronicotinyl insecticide imidacloprid was developed using a polyclonal antibody produced against a hapten conjugated through the imidazolidine to keyhole limpet hemocyanin. In the standard curve of imidacloprid, an IC50 of 17.3 ng/mL was obtained using a competitive heterologous system at pH 10. Very low crossreactivity was found for some structurally related compounds including the insecticide thiacloprid. The high cross-reactivity with a metabolite containing the carbonyl group in the imidazolidine moiety suggests the involvement of its polarity and stereochemical fitness in forming the antibody-antigen complex. The effects of various assay conditions, including organic solvents, detergent content, salt concentration, and pH on the sensitivity,were evaluated. High-performance liquid chromatography was run for comparison to validate the ELISA with fortified water samples, the correlation being 0.997-0.998 (n = 15) with a slope of 1.10-1.38. The ELISA turned out to be a convenient tool for monitoring imidacloprid residues in agricultural and environmental samples.

  DOI：

  10.1021/jf001140v
• 作为产物：
  描述：

  吡虫啉 在 铁粉 、 氯化铵 作用下， 以 乙醇 为溶剂， 反应 39.0h， 以60.5%的产率得到1-(6-chloro-3-pyridylmethyl)-N-aminoimidazolidin-2-ylideneamine
  参考文献：
  名称：

  Development of an ELISA for the Detection of the Residues of the Insecticide Imidacloprid in Agricultural and Environmental Samples

  摘要：

  A competitive enzyme-linked immunosorbent assay (ELISA) for the chloronicotinyl insecticide imidacloprid was developed using a polyclonal antibody produced against a hapten conjugated through the imidazolidine to keyhole limpet hemocyanin. In the standard curve of imidacloprid, an IC50 of 17.3 ng/mL was obtained using a competitive heterologous system at pH 10. Very low crossreactivity was found for some structurally related compounds including the insecticide thiacloprid. The high cross-reactivity with a metabolite containing the carbonyl group in the imidazolidine moiety suggests the involvement of its polarity and stereochemical fitness in forming the antibody-antigen complex. The effects of various assay conditions, including organic solvents, detergent content, salt concentration, and pH on the sensitivity,were evaluated. High-performance liquid chromatography was run for comparison to validate the ELISA with fortified water samples, the correlation being 0.997-0.998 (n = 15) with a slope of 1.10-1.38. The ELISA turned out to be a convenient tool for monitoring imidacloprid residues in agricultural and environmental samples.

  DOI：

  10.1021/jf001140v

文献信息

• Development of an ELISA for the Detection of the Residues of the Insecticide Imidacloprid in Agricultural and Environmental Samples
  作者：Jae Koo Lee、Ki Chang Ahn、Oee Suk Park、Shin Young Kang、Bruce D. Hammock

  DOI：10.1021/jf001140v

  日期：2001.5.1

  A competitive enzyme-linked immunosorbent assay (ELISA) for the chloronicotinyl insecticide imidacloprid was developed using a polyclonal antibody produced against a hapten conjugated through the imidazolidine to keyhole limpet hemocyanin. In the standard curve of imidacloprid, an IC50 of 17.3 ng/mL was obtained using a competitive heterologous system at pH 10. Very low crossreactivity was found for some structurally related compounds including the insecticide thiacloprid. The high cross-reactivity with a metabolite containing the carbonyl group in the imidazolidine moiety suggests the involvement of its polarity and stereochemical fitness in forming the antibody-antigen complex. The effects of various assay conditions, including organic solvents, detergent content, salt concentration, and pH on the sensitivity,were evaluated. High-performance liquid chromatography was run for comparison to validate the ELISA with fortified water samples, the correlation being 0.997-0.998 (n = 15) with a slope of 1.10-1.38. The ELISA turned out to be a convenient tool for monitoring imidacloprid residues in agricultural and environmental samples.