N-((6-(Benzyloxy)purin-9-yl) acetyl)-N-(2-Boc-aminoethyl)glycine

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 英文名称: N-((6-(Benzyloxy)purin-9-yl) acetyl)-N-(2-Boc-aminoethyl)glycine
• 英文别名: 2-[2-[(2-Methylpropan-2-yl)oxycarbonylamino]ethyl-[2-(6-phenylmethoxypurin-9-yl)acetyl]amino]acetic acid
• 化学式: C₂₃H₂₈N₆O₆
• 分子量: 484.512
• InChiKey: NWJPSOOJLPXRRU-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  1.8
• 重原子数：
  35
• 可旋转键数：
  12
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.39
• 拓扑面积：
  149
• 氢给体数：
  2
• 氢受体数：
  9

反应信息

• 作为产物：
  描述：

  2-(6-(benzyloxy)-9H-purin-9-yl)acetic acid 、 N-[2-(叔丁氧羰基氨基)乙基]甘氨酸乙酯盐酸盐 、 3-羟基-1,2,3-苯并三嗪-4(3H)-酮 以 甲醇 、 二氯甲烷 、 水 为溶剂， 生成 N-((6-(Benzyloxy)purin-9-yl) acetyl)-N-(2-Boc-aminoethyl)glycine 、 次黄嘌呤
  参考文献：
  名称：

  IMMUNOHISTOCHEMISTRY DETECTION METHOD

  摘要：

  公开号：

  EP1910575B1

文献信息

• NUCLEIC ACID BASE PAIRS
  申请人：Lohse Jesper

  公开号：US20100105030A1

  公开（公告）日：2010-04-29

  The invention relates to non-natural bases and base pairs that expand the normal DNA-based encoding system. Compositions herein may comprise at least one non-natural base that may interact with another base via a Watson Crick-type hydrogen bonding geometry and/or a Hoogsteen-type hydrogen bonding geometry. The bases may be used in a molecular entity, such as an oligomer or any other entity wherein the bases are attached to a backbone. For example, they may be comprised in DNA, RNA, or PNA, or a variety of other nucleic acid-type systems.

  本发明涉及扩展正常基于DNA编码系统的非自然碱基和碱基对。本文中的组合物可能包含至少一种非自然碱基，该碱基可以通过Watson Crick型氢键几何和/或Hoogsteen型氢键几何与另一碱基相互作用。这些碱基可以用于分子实体，例如寡聚物或任何其他碱基附着于骨架的实体。例如，它们可以包含在DNA、RNA或PNA中，或者各种其他核酸类型系统中。
• METHOD OF SIMULTANEOUSLY VISUALIZING MULTIPLE BIOLOGICAL TARGETS
  申请人：Winther Lars

  公开号：US20100105145A1

  公开（公告）日：2010-04-29

  The present invention relates to optionally automated methods that may be used to qualitatively and/or quantitatively detect at least one, or for example, two or more different targets in a sample, and kits associated with such methods. The two or more different targets may be detected and distinguished by adding at least one cross-linking agent to the sample in between different steps of a detection procedure. The addition of a cross-linking agent may allow for drastic changes in buffer conditions (i.e. solvent, pH, salt concentration, etc.) or temperature in order to refine a detection procedure with minimal loss of signal. The instant invention is compatible with a variety of detection systems, including immunohistochemistry (IHC), immunocytochemistry (ICC), in situ hybridization (ISH), flow cytometry, enzyme immuno-assays (EIA), enzyme linked immuno-assays (ELISA), blotting methods (e.g. Western, Southern, and Northern), labeling inside electrophoresis systems or on surfaces or arrays, and precipitation, among other general detection assay formats. The invention is also compatible with many different types of samples, targets, probes, and detectable labels.

  本发明涉及可选自动化方法，用于在样品中定性和/或定量检测至少一个不同的目标或例如两个或更多不同的目标，并且与此类方法相关联的试剂盒。可以通过在检测过程的不同步骤之间添加至少一种交联剂来检测和区分两个或更多不同的目标。添加交联剂可以允许在最小信号损失的情况下在缓冲条件（即溶剂，pH，盐浓度等）或温度方面进行巨大的改变，以精细检测程序。本发明与各种检测系统兼容，包括免疫组织化学（IHC），免疫细胞化学（ICC），原位杂交（ISH），流式细胞术，酶免疫分析（EIA），酶联免疫分析（ELISA），印迹方法（例如Western，Southern和Northern），在电泳系统内或表面或阵列上标记，以及沉淀等其他一般检测分析格式。本发明还与许多不同类型的样品，目标，探针和可检测标签兼容。
• MONOMERIC AND POLYMERIC LINKERS USEFUL FOR CONJUGATING BIOLOGICAL MOLECULES AND OTHER SUBSTANCES
  申请人：Lohse Jesper

  公开号：US20100120054A1

  公开（公告）日：2010-05-13

  The present invention concerns monomeric or polymeric linker molecules useful in biological and chemical applications, their synthesis, and the synthesis and use of derivatives of the linkers conjugated to a variety of detectable labels and other substances. The linkers may be used, for example, in conjunction with fluorescent labels, nucleic acid or nucleic acid analog probes, and solid phase systems, and to enhance the solubility of the conjugated molecules.

  本发明涉及单体或聚合链连接分子，可用于生物和化学应用，其合成以及连接物的衍生物的合成和使用，这些连接物与各种可检测标记和其他物质结合。例如，这些连接物可以与荧光标记、核酸或核酸类似物探针、固相系统一起使用，以及增强连接分子的溶解度。
• IN SITU HYBRIDIZATION DETECTION METHOD
  申请人：Winther Lars

  公开号：US20100221705A1

  公开（公告）日：2010-09-02

  The invention provides compositions and methods for the detection of targets in a sample; in particular, an in situ hybridization (ISH) sample. Probes and detectable labels may be provided in multiple layers in order to increase the flexibility of a detection system, and to allow for amplification to enhance the signal from a target. The layers may be created by incorporating probes and detectable labels into larger molecular units that interact through nucleic acids base-pairing, including peptide-nucleic acid (PNA) base-pairing. Optional non-natural bases allow for degenerate base pairing schemes. The compositions and methods are also compatible with immunohistochemistry (IHC), immunocytochemistry (ICC), flow cytometry, enzyme immuno-assays (EIA), enzyme linked immuno-assays (ELISA), blotting methods (e.g. Western, Southern, and Northern), labeling inside electrophoresis systems or on surfaces or arrays, and precipitation, among other general detection assay formats. The invention is also compatible with many different types of targets, probes, and detectable labels.

  本发明提供了一种用于检测样品中的目标物的组合物和方法；特别是用于原位杂交（ISH）样品的检测。探针和可检测标记可以在多个层面上提供，以增加检测系统的灵活性，并允许扩增以增强目标物的信号。这些层可以通过将探针和可检测标记合并到较大的分子单元中而创建，这些分子单元通过核酸碱基配对相互作用，包括肽核酸（PNA）碱基配对。可选的非天然碱基允许退化碱基配对方案。这些组合物和方法也与免疫组织化学（IHC）、免疫细胞化学（ICC）、流式细胞术、酶免疫分析（EIA）、酶联免疫分析（ELISA）、印迹方法（例如Western、Southern和Northern）、在电泳系统内或在表面或阵列上标记以及沉淀等其他常见的检测测定格式兼容。本发明也与许多不同类型的目标物、探针和可检测标记兼容。
• Systems and Methods for Biological and Chemical Detection, Comprising Automatic Selection of Reagent Sets
  申请人：Winther Lars

  公开号：US20100151460A1

  公开（公告）日：2010-06-17

  The instant invention relates to systems of reagents and methods for the detection of chemical and biological targets in a sample. Some embodiments comprise methods for automatically selecting a set of reagents to detect one or more targets in a sample, wherein the set of reagents comprises at least two layers for detection of a first target, and at least two layers for detection of a second target, wherein the set comprises reagents that are at least partially redundant. In some embodiments, the redundancy is created by at least one degenerate reagent such that the reagent may interact specifically with more than one other component of a detection system or sample. In some embodiments, the system or method also includes reagent containers with a computer-generated code which may further serve to match targets to appropriate reagents.

  本发明涉及一种试剂体系和检测样品中化学和生物目标的方法。某些实施例包括自动选择一组试剂以检测样品中的一个或多个目标的方法，其中试剂组包括至少两层用于检测第一个目标的试剂和至少两层用于检测第二个目标的试剂，其中试剂组包括至少部分冗余的试剂。在某些实施例中，冗余性是通过至少一种退化试剂创建的，以使试剂可以与检测系统或样品的多个其他组分特异地相互作用。在某些实施例中，该系统或方法还包括带有计算机生成的代码的试剂容器，该代码可以进一步用于将目标与适当的试剂匹配。