hydroxyisobutyryl lysine

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 英文名称: hydroxyisobutyryl lysine
• 英文别名: N(6)-(2-hydroxyisobutyryl)-L-lysine；(2S)-2-amino-6-[(2-hydroxy-2-methylpropanoyl)amino]hexanoic acid
• 化学式: C₁₀H₂₀N₂O₄
• 分子量: 232.28
• InChiKey: SVPGURLIUMTWMM-ZETCQYMHSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -3
• 重原子数：
  16
• 可旋转键数：
  7
• 环数：
  0.0
• sp3杂化的碳原子比例：
  0.8
• 拓扑面积：
  113
• 氢给体数：
  4
• 氢受体数：
  5

反应信息

• 作为产物：
  描述：

  (2,5-Dioxopyrrolidin-1-yl) 2-hydroxy-2-methylpropanoate 、 L-赖氨酸盐酸盐 在 碳酸氢钠 、 copper(II) sulfate 作用下， 以 丙酮 为溶剂， 反应 4.17h， 以77%的产率得到hydroxyisobutyryl lysine
  参考文献：
  名称：

  Genetic encoding of the post-translational modification 2-hydroxyisobutyryl-lysine

  摘要：

  报道将2-羟基异丁酰赖氨酸添加到大肠杆菌的遗传密码中。

  DOI：

  10.1039/c5ob00283d

文献信息

• [EN] METHODS AND KITS FOR DETERMINING DEACYLASE ACTIVITY<br/>[FR] PROCÉDÉS ET KITS DE DÉTERMINATION D'UNE ACTIVITÉ DE DÉSACYLASE
  申请人：THE NAT INST FOR BIOTECHNOLOGY IN THE NEGEV LTD

  公开号：WO2017064718A1

  公开（公告）日：2017-04-20

  Methods and kits for determining deacylase activity on an acylated lysine residue of a target polypeptide are disclosed. The methods disclosed comprise: providing one or more bacterial cells comprising: (i) an orthogonal pair of tRNA synthetase and tRNA; and/or (ii) a polynucleotide sequence encoding a target polypeptide, wherein said polynucleotide sequence comprises at least one codon that is specifically recognized by said tRNA; and (iii) at least one mammalian deacetylase; incubating said bacterial cell or extract thereof in the presence of Nɛ-acetyl lysine, wherein said tRNA is aminoacylated with Nɛ-modified lysine and incorporates the Nɛ-modified lysine into said target polypeptide, thereby expressing said target polypeptide comprising an Nɛ-modified lysine residue; determining the acylation levels of said target polypeptide; and comparing said acylation levels to a control; thereby determining deacylase activity.
• Genetic encoding of the post-translational modification 2-hydroxyisobutyryl-lysine
  作者：William A. Knight、T. Ashton Cropp

  DOI：10.1039/c5ob00283d

  日期：——

  Adding 2-hydroxyisobutyryl lysine to the genetic code of E. coli is reported.

  报道将2-羟基异丁酰赖氨酸添加到大肠杆菌的遗传密码中。