1-trifluoroacetamido-oct-7-yn

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 英文名称: 1-trifluoroacetamido-oct-7-yn
• 英文别名: 8-trifluoroacetamido-1-octyne；2,2,2-trifluoro-N-oct-7-ynylacetamide
• 化学式: C₁₀H₁₄F₃NO
• 分子量: 221.222
• InChiKey: CBVUSWPETUMUBR-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  3
• 重原子数：
  15
• 可旋转键数：
  6
• 环数：
  0.0
• sp3杂化的碳原子比例：
  0.7
• 拓扑面积：
  29.1
• 氢给体数：
  1
• 氢受体数：
  4

反应信息

• 作为反应物：
  描述：

  3'-O-bezoyl-2'-deoxy-5-iodouridine 、 1-trifluoroacetamido-oct-7-yn 在 copper(l) iodide 、 tris(triphenylphosphine)-palladium(II) dichloride 作用下， 以 三乙胺 为溶剂， 反应 2.0h， 以85%的产率得到3'-O-benzoyl-2'-deoxy-5-(8-N-trifluoroacetamido-oct-1-ynyl)-uridine
  参考文献：
  名称：

  Syntheses of inhibitors for the enzyme thymidine 5′-diphosphate-glucose-4,6-dehydratase (EC 4.2.1.46)

  摘要：

  Inhibitors of the enzyme thymidine 5'-diphosphate-glucose-4,6-dehydratase (EC 4.2.1.46) were synthesised starting from thymidine, 2-deoxy-uridine and -cytidine. The diphosphate moieties have either been replaced by methylene diphosphonate or phosphoryl hydroxyacetic acid to yield inhibitors in a similar range to thymidine 5'-diphosphate itself. Spacers were attached to various positions, of which those at C-5 of the nucleobase were suitable analogues and showed a mixed type of inhibition characteristics, whereas attachment at other sites led to marginally active or inactive inhibitors. These investigations will prepare the ground for developing a purification procedure based on affinity chromatography. (C) 1999 Elsevier Science Ltd. All rights reserved.

  DOI：

  10.1016/s0008-6215(99)00074-9
• 作为产物：
  描述：

  2,2,2-三氟乙酰胺 、 8-iodooct-1-yne 、 ammonium chloride 以 N,N-二甲基甲酰胺 为溶剂， 以79.3%的产率得到1-trifluoroacetamido-oct-7-yn
  参考文献：
  名称：

  3'-Deoxyribonucleotide derivatives

  摘要：

  披露了由以下一般公式表示的3′-脱氧核苷酸衍生物：Q—V—(CH2)n—NH—R 其中 Q代表一个3′-脱氧核苷酸残基，n代表不小于4的整数，V代表—C≡C—或—CH═CH—，R代表一个荧光基团。上述3′-脱氧核苷酸衍生物是具有改善RNA聚合酶使用速率的衍生物，可用作DNA序列测定方法中利用RNA聚合酶的终止子。

  公开号：

  US06265569B1

文献信息

• Method of DNA sequencing
  申请人：The Institute of Physical and Chemical Research

  公开号：US06365350B1

  公开（公告）日：2002-04-02

  Disclosed are methods for determining DNA nucleotide sequences comprising reacting ribonucleoside 5′-triphosphates and 3′-dNTP derivatives in the presence of an RNA polymerase modified so as to enhance its ability for incorporating the 3′-dNTP derivatives and a DNA fragment containing a promoter sequence for the RNA polymerase to obtain a nucleic acid transcription product, separating the resulting nucleic acid transcription product, and determining a nucleic acid sequence from the resulting separated fraction. These methods can produce a transcription product of a long chain and afford more accurate sequence data where fluctuation of signals from labeled deoxyribonucleotides is reduced.

  揭示了一种确定DNA核苷酸序列的方法，包括在存在已经修改以增强其用于将3′-dNTP衍生物合并的RNA聚合酶的情况下，反应核苷酸5′-三磷酸酯和3′-dNTP衍生物，以及包含用于RNA聚合酶的启动子序列的DNA片段，以获得核酸转录产物，分离所得的核酸转录产物，并从分离的分数中确定核酸序列。这些方法可以产生长链的转录产物，并提供更准确的序列数据，减少了标记的脱氧核糖核苷酸信号波动。
• METHOD OF DNA SEQUENCING
  申请人：THE INSTITUTE OF PHYSICAL & CHEMICAL RESEARCH

  公开号：EP0978569A1

  公开（公告）日：2000-02-09

  A method of DNA sequencing comprising reacting a ribonucleoside 5'-triphosphate with a 3'dNTP derivative in the presence of a mutated RNA polymerase modified so as to enhance the ability to take up the 3'dNTP derivative and a DNA fragment containing a promoter sequence for the RNA polymerase, separating the nucleic acid transcription product thus obtained, and reading the sequence of the nucleic acid from the fraction thus separated. By using this method, a long-chain transcription product can be formed and more accurate sequence data with little change in the signals from labeled deoxyribonucleotides can be obtained.

  一种 DNA 测序方法，包括在经过修饰以提高吸收 3'dNTP 衍生物能力的突变 RNA 聚合酶和含有 RNA 聚合酶启动子序列的 DNA 片段存在的情况下，使核糖核苷 5'- 三磷酸酯与 3'dNTP 衍生物反应，分离由此获得的核酸转录产物，并从由此分离的部分读取核酸序列。使用这种方法可以形成长链转录产物，获得更准确的序列数据，而标记脱氧核苷酸的信号变化很小。
• 3'-DEOXYRIBONUCLEOTIDE DERIVATIVES
  申请人：THE INSTITUTE OF PHYSICAL & CHEMICAL RESEARCH

  公开号：EP1046648A1

  公开（公告）日：2000-10-25

  3'-Deoxyribonucleotide derivatives represented by the following general formula [I]: Q-V-(CH2)n-NR-R wherein Q represents a 3'-deoxyribonucleotide residue; n is an integer of 4 or more; V represents -C≡C- or -CH=CH-; and R represents a fluorescent group. Because of having been improved in the uptake efficiency by RNA polymerases, these derivatives are useful as terminators in DNA base sequencing methods with the use of RNA polymerases.

  由以下通式[I]代表的 3'-脱氧核苷酸衍生物：Q-V-(CH2)n-NR-R 其中 Q 代表 3'-脱氧核苷酸残基；n 是 4 或更多的整数；V 代表 -C≡C- 或 -CH=CH-；R 代表荧光基团。由于提高了 RNA 聚合酶的吸收效率，这些衍生物在使用 RNA 聚合酶的 DNA 碱基测序方法中可用作终止子。
• US6265569B1
  申请人：——

  公开号：US6265569B1

  公开（公告）日：2001-07-24
• US6365350B1
  申请人：——

  公开号：US6365350B1

  公开（公告）日：2002-04-02