15-(2,4-dinitrophenylamino)-4,7,10,13-tetraoxapentadecanoic acid

• 分子结构分类: 有机化合物 - 苯类化合物 - 苯和取代衍生物
• 英文名称: 15-(2,4-dinitrophenylamino)-4,7,10,13-tetraoxapentadecanoic acid
• 英文别名: DNP-PEG4-CO2H；3-[2-(2-{2-[2-(2,4-dinitrophenylamino)ethoxy]ethoxy}ethoxy)ethoxy]propanoic acid；DNP-dPEG4-CO2H；DNP-PEG4-acid；3-[2-[2-[2-[2-(2,4-dinitroanilino)ethoxy]ethoxy]ethoxy]ethoxy]propanoic acid
• 化学式: C₁₇H₂₅N₃O₁₀
• 分子量: 431.4
• InChiKey: PTIFQVOBASTCFB-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  1.1
• 重原子数：
  30
• 可旋转键数：
  16
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.59
• 拓扑面积：
  178
• 氢给体数：
  2
• 氢受体数：
  11

反应信息

• 作为反应物：
  描述：

  15-(2,4-dinitrophenylamino)-4,7,10,13-tetraoxapentadecanoic acid 在 4-二甲氨基吡啶 、 碳酸氢钠 、 sodium hydroxide 作用下， 以 水 、 乙腈 为溶剂， 反应 1.75h， 生成 N,N'-(15-(2,4-dinitrophenylamino)-4,7,10,13-tetraoxapentadecanoyl)-L-cystine
  参考文献：
  名称：

  [EN] REAGENTS FOR THIOL CONJUGATION AND CONJUGATES FORMED THEREFROM
  [FR] RÉACTIFS DE CONJUGAISON THIOL ET CONJUGUÉS FORMÉS À PARTIR DE CEUX-CI

  摘要：

  用于制备稳定的硫醚以进行体内给药的试剂已经公开，以及因此制备的这些硫醚。

  公开号：

  WO2016025752A1
• 作为产物：
  描述：

  3-[2-[2-[2-(2-氨基乙氧基)乙氧基]乙氧基]乙氧基]丙酸 、 2,4-二硝基氟苯 在 sodium carbonate 、 盐酸 作用下， 以 水 为溶剂， 以92%的产率得到15-(2,4-dinitrophenylamino)-4,7,10,13-tetraoxapentadecanoic acid
  参考文献：
  名称：

  Microwave-Mediated Synthesis of Labeled Nucleotides with Utility in the Synthesis of DNA Probes

  摘要：

  A novel method of linking haptens to deoxycytidine 5'-triphosphate via microwave-mediated bisulfate-catalyzed transamination with hydrazine has been developed. This method enables the tethering of small molecule haptens to dCTP via a discrete polyethylene glycol (PEG) spacer, yielding N-4-aminodeoxycytidine 5'-triphosphate-dPEG-haptens. This synthetic approach employs microwave-catalyzed hydrazinolysis that enables the attachment of spacers via hydrazine linkages. The microwave-mediated introduction of this hydrazine handle provides a significant improvement in yield over those of published thermal methods. The microwave reaction was shown to be scalable, and the final product was amenable to labeling with a wide variety of haptens. The resulting nucleotide triphosphates, N-4-aminodeoxycytidine 5'-triphosphate-dPEG-haptens, can serve as unique substrates for the enzyme-mediated labeling of DNA probes. The efficacy of incorporation of one such novel nucleotide, N-4-aminodeoxycyticline 5'-triphosphate-dPEG(4)-DNP, has been demonstrated in nick translation labeling of HER2 and HPV probes. The labeled probes have been shown to be effective in visualizing their target genes in tissue.

  DOI：

  10.1021/bc100013b

文献信息

• Microwave mediated synthesis of nucleic acid probes
  申请人：Bieniarz Christopher

  公开号：US20050158770A1

  公开（公告）日：2005-07-21

  A method for preparing a nucleic acid probe is provided. The method comprises forming an activated cytosine or cytidine by a bisulfite catalyzed reaction; and covalently linking a reporter molecule to the activated cytosine or cytidine, wherein said activating step, said covalently linking step, or both are conducted in the presence of microwave energy. Also provided by the invention are nucleic acid probes.

  提供了一种制备核酸探针的方法。该方法包括通过亚硫酸氢盐催化反应形成活化的胞嘧啶或胞苷；以及将报告分子与活化的胞嘧啶或胞苷共价连接，其中所述活化步骤、所述共价连接步骤或两者均在微波能量存在下进行。本发明还提供了核酸探针。
• US7541455B2
  申请人：——

  公开号：US7541455B2

  公开（公告）日：2009-06-02
• WO2022/178633
  申请人：——

  公开号：——

  公开（公告）日：——