(E)-2-oxo-5-(carboxymethyl)-3-hexenedioate

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 英文名称: (E)-2-oxo-5-(carboxymethyl)-3-hexenedioate
• 英文别名: (3E)-5-oxopent-3-ene-1,2,5-tricarboxylic acid；(E)-5-oxopent-3-ene-1,2,5-tricarboxylic acid
• 化学式: C₈H₈O₇
• 分子量: 216.147
• InChiKey: WHGVLEMQINVDLH-OWOJBTEDSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -0.9
• 重原子数：
  15
• 可旋转键数：
  6
• 环数：
  0.0
• sp3杂化的碳原子比例：
  0.25
• 拓扑面积：
  129
• 氢给体数：
  3
• 氢受体数：
  7

反应信息

• 作为产物：
  描述：

  5-(carboxymethyl)-2-hydroxymuconic acid 在 sodium dihydrogenphosphate 作用下， 生成 (E)-2-oxo-5-(carboxymethyl)-3-hexenedioate
  参考文献：
  名称：

  4-草酰巴豆酸互变异构酶和 5-(羧甲基)-2-羟基粘康酸异构酶对 2-羟基粘康酸的立体特异性酮化

  摘要：

  来自恶臭假单胞菌 mt-2 的 4-草酰巴豆酸互变异构酶和来自大肠杆菌 C 的 5-(羧甲基)-2-羟基粘康酸异构酶在仅羧甲基不同的底物上催化相同的反应。虽然这两种蛋白质的底物之间的结构相似性表明这些酶可能在进化上相关，但现有文献并未表明存在明显的联系。发现这两种酶都使 2-羟基粘康酸 (1) - 4-草酰巴豆酸互变异构酶的底物酮化

  DOI：

  10.1021/ja00052a002

文献信息

• Probe compound for detecting and isolating enzymes and means and methods using the same
  申请人：Helmholtz-Zentrum für Infektionsforschung GmbH

  公开号：EP2230312A1

  公开（公告）日：2010-09-22

  The present invention relates to a probe compound that can comprise any substrate or metabolite of an enzymatic reaction in addition to an indicator component, such as, for example, a fluorescence dye, or the like. Moreover, the present invention relates to means for detecting enzymes in form of an array, which comprises any number of probe compounds of the invention which each comprise a different metabolite of interconnected metabolites representing the central pathways in all forms of life. Moreover, the present invention relates to a method for detecting enzymes involving the application of cell extracts or the like to the array of the invention which leads to reproducible enzymatic reactions with the substrates. These specific enzymatic reactions trigger the indicator (e.g. a fluorescence signal) and bind the enzymes to the respective cognate substrates. Moreover, the invention relates to means for isolating enzymes in form of nanoparticles coated with the probe compound of the invention. The immobilisation of the cognate substrates or metabolites on the surface of nanoparticles by means of the probe compounds allows capturing and isolating the respective enzyme, e.g. for subsequent sequencing.

  本发明涉及一种探针化合物，它可以包括酶反应的任何底物或代谢物，此外还包括指示成分，例如荧光染料或类似物。此外，本发明还涉及以阵列形式检测酶的方法，该阵列由任意数量的本发明探针化合物组成，每种探针化合物由代表所有生命形式中中心途径的相互关联的代谢物中的不同代谢物组成。此外，本发明还涉及一种检测酶的方法，该方法涉及将细胞提取物或类似物应用于本发明的阵列，从而导致与底物发生可重复的酶反应。这些特定的酶反应会触发指示剂（如荧光信号），并将酶与各自的同源底物结合。此外，本发明还涉及以涂覆有本发明探针化合物的纳米颗粒形式分离酶的方法。通过探针化合物将同源底物或代谢物固定在纳米颗粒表面，可以捕获和分离相应的酶，例如用于后续测序。
• Methods for producing isobutene from 3-methylcrotonic acid
  申请人：Global Bioenergies

  公开号：US11124806B2

  公开（公告）日：2021-09-21

  Described are methods for the production of isobutene comprising the enzymatic conversion of 3-methylcrotonic acid into isobutene wherein said 3-methylcrotonic acid is obtained by the enzymatic conversion of 3-methylcrotonyl-CoA into 3-methylcrotonic acid or wherein said 3-methylcrotonic acid is obtained by the enzymatic conversion of 3-hydroxyisovalerate (HIV) into 3-methylcrotonic acid. It is described that the enzymatic conversion of 3-methylcrotonic acid into isobutene can, e.g., be achieved by making use of a 3-methylcrotonic acid decarboxylase, preferably an FMN-dependent decarboxylase associated with an FMN prenyl transferase, an aconitate decarboxylase (EC 4.1.1.6), a methylcrotonyl-CoA carboxylase (EC 6.4.1.4), or a geranoyl-CoA carboxylase (EC 6.4.1.5).

  所述异丁烯的生产方法包括酶法将 3-甲基巴豆酸转化为异丁烯，其中所述 3-甲基巴豆酸是通过酶法将 3-甲基巴豆酰-CoA 转化为 3-甲基巴豆酸得到的，或者所述 3-甲基巴豆酸是通过酶法将 3-羟基异戊酸（HIV）转化为 3-甲基巴豆酸得到的。据介绍，将 3-甲基巴豆酸酶法转化为异丁烯的过程可通过以下方式实现，例如3-甲基巴豆酸脱羧酶，最好是与 FMN 前酰基转移酶相关的 FMN 依赖性脱羧酶、丙酮酸脱羧酶（EC 4.1.1.6）、甲基巴豆酰-CoA 羧化酶（EC 6.4.1.4）或香叶酰-CoA 羧化酶（EC 6.4.1.5）。
• Human peptidyl-prolyl isomerases
  申请人：Incyte Genomics, Inc.

  公开号：US20030087390A1

  公开（公告）日：2003-05-08

  The invention provides human peptidyl-prolyl isomerases (HPPIP) and polynucleotides which identify and encode HPPIP. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists. The invention also provides methods for diagnosing, treating or preventing disorders associated with expression of HPPIP.

  本发明提供了人肽基脯氨酰异构酶（HPPIP）以及识别和编码 HPPIP 的多核苷酸。本发明还提供了表达载体、宿主细胞、抗体、激动剂和拮抗剂。本发明还提供了诊断、治疗或预防与 HPPIP 表达有关的疾病的方法。
• Hajipour, Gholamhossein; Johnson Jr., William H.; Dauben, Paul D., Journal of the American Chemical Society, 1993, vol. 115, # 9, p. 3533 - 3542
  作者：Hajipour, Gholamhossein、Johnson Jr., William H.、Dauben, Paul D.、Stolowich, Neal J.、Whitman, Christian P.

  DOI：——

  日期：——
• DRUG METABOLIZING ENZYMES
  申请人：Incyte Genomics, Inc.

  公开号：EP1254236A2

  公开（公告）日：2002-11-06