2,4-二[2-[4-(二甲基氨基)苯基]乙烯基]-1-乙基吡啶鎓碘化物 | 84195-77-7

• 分子结构分类: 有机化合物 - 苯丙烷和聚酮 - 二芳基庚烷
• 中文名称: 2,4-二[2-[4-(二甲基氨基)苯基]乙烯基]-1-乙基吡啶鎓碘化物
• 英文名称: 1-ethyl-2,4-bis-(4-dimethylamino-trans-styryl)-pyridinium; iodide
• 英文别名: 1-Aethyl-2,4-bis-(4-dimethylamino-trans-styryl)-pyridinium; Jodid；2,4-Bis{2-[4-(dimethylamino)phenyl]ethenyl}-1-ethylpyridin-1-ium iodide；4-[2-[2-[2-[4-(dimethylamino)phenyl]ethenyl]-1-ethylpyridin-1-ium-4-yl]ethenyl]-N,N-dimethylaniline;iodide
• CAS: 84195-77-7
• 化学式: C₂₇H₃₂N₃*I
• 分子量: 525.476
• InChiKey: XPRYLWBGHVPGNC-UHFFFAOYSA-M

计算性质

• 辛醇/水分配系数(LogP)：
  2.47
• 重原子数：
  31
• 可旋转键数：
  7
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.22
• 拓扑面积：
  10.4
• 氢给体数：
  0
• 氢受体数：
  3

反应信息

• 作为产物：
  描述：

  对二甲氨基苯甲醛 、 alkaline earth salt of/the/ methylsulfuric acid 在 哌啶 作用下， 生成 2,4-二[2-[4-(二甲基氨基)苯基]乙烯基]-1-乙基吡啶鎓碘化物
  参考文献：
  名称：

  The Absorption Spectra of Styryl Dyes

  摘要：

  DOI：

  10.1246/bcsj.26.105

文献信息

• Method of classifying leucocytes by flow cytometry
  申请人：TOA MEDICAL ELECTRONICS CO., LTD.

  公开号：EP0259834A2

  公开（公告）日：1988-03-16

  A method for classifying leukocytes with a flow cytometer by means of optical measurements on fluorochrome-­stained blood cells is useful in the practice of clinical testing.

  流式细胞仪通过对荧光染色的血细胞进行光学测量来对白细胞进行分类的方法在临床检测中非常有用。
• Method of differentiating erythroblasts from other cells by flow cytometry
  申请人：TOA MEDICAL ELECTRONICS CO., LTD.

  公开号：EP0499693A2

  公开（公告）日：1992-08-26

  A method which comprises using a specified dye taken up by erythrocytic nucleated cells so that their nuclei are stained and can be readily differentiated from other cells by measurement with a flow cytometer is disclosed. The method for differentiating erythroblasts from other cells relies basically upon flow cytometry by two-step staining that uses a first fluid that is an acidic hypotensive fluorescent dye solution and a second fluid that is a solution that changes the osmorality and pH of the first fluid. The first fluid further contains a fluorescent dye that diffuses into erythroblasts to specifically stain their nuclei, and a group of erythroblasts is separated from other cell groups on the two-dimensional plot constructed with the flow cytometer, whereby the results of erythroblast differentiation are computed.

  本发明公开了一种方法，该方法包括使用一种被红细胞有核细胞吸收的特定染料，使其细胞核被染色，并可通过流式细胞仪的测量将其与其他细胞区分开来。 这种区分红细胞和其他细胞的方法主要依靠流式细胞仪的两步染色法，使用的第一种液体是酸性低渗透性荧光染料溶液，第二种液体是改变第一种液体渗透性和 pH 值的溶液。第一种液体中还含有一种荧光染料，这种染料会扩散到红细胞中，对其细胞核进行特异性染色，然后在用流式细胞仪绘制的二维图上，将一组红细胞与其他细胞组分开，从而计算出红细胞分化的结果。
• Method of classifying leukocytes by flow cytometry
  申请人：TOA MEDICAL ELECTRONICS CO., LTD.

  公开号：EP0485945A2

  公开（公告）日：1992-05-20

  A method for leukocyte classification which relies basically upon flow cytometry that uses a first fluid that is hypotonic acidic fluorescent dye solution and a second fluid that is a solution that changes the osmolarity and pH of the first fluid is disclosed. In accordance with the method, a fluorescent dye that specifically stains the nuclei of damaged leukocyte cells is added to the first fluid and the resulting fluid is used in measurements. The first fluid to be used in the method incorporates not only the first and second dyes but also the third dye and this insures that even leukocytes with damaged cell membrane can be distributed as entities distinct from other leukocyte types on a two-dimensional plot. And, hence, high precision of leukocyte classification is assured even in the case of assaying blood that contains damaged cells as a result of prolonged standing at room temperature after sampling.

  本发明公开了一种白细胞分类方法，该方法主要依靠流式细胞术，使用的第一种液体是低渗酸性荧光染料溶液，第二种液体是改变第一种液体渗透压和 pH 值的溶液。根据该方法，可特异性染色受损白细胞细胞核的荧光染料被添加到第一种液体中，所得液体用于测量。 该方法中使用的第一种液体不仅含有第一种和第二种染料，还含有第三种染料，这就确保了即使是细胞膜受损的白细胞也能作为不同于其他类型白细胞的实体分布在二维图上。因此，即使在检测因采样后在室温下长时间放置而含有受损细胞的血液时，也能确保白细胞分类的高精度。
• Reagent and method for analyzing cells in urine
  申请人：TOA MEDICAL ELECTRONICS CO., LTD.

  公开号：EP0513762A1

  公开（公告）日：1992-11-19

  A reagent for analyzing cells in urine and a method for analyzing cells in urine with the use of said reagent are provided. The present invention discloses a reagent for analyzing cells in urine which comprises solution(s) containing a fluorescent dye, an osmolarity compensating agent and a buffer, as well as a method for analyzing cells in urine which comprises diluting a urine sample and staining cells therein with said reagent, irradiating said cells with light in the violet or blue wavelength region by using a flow cytometer and measuring the forward or side scattered light and fluorescence from said cells.

  本发明提供了一种用于分析尿液中细胞的试剂和一种利用上述试剂分析尿液中细胞的方法。本发明公开了一种用于分析尿液中细胞的试剂，该试剂由含有荧光染料、渗透压补偿剂和缓冲液的溶液组成；还公开了一种用于分析尿液中细胞的方法，该方法包括稀释尿液样本并用所述试剂对其中的细胞进行染色，使用流式细胞仪用紫光或蓝光波长区域的光照射所述细胞，并测量所述细胞发出的正向或侧向散射光和荧光。
• Process for bringing about separation of individual microorganisms, and applications of that process
  申请人：CANON KABUSHIKI KAISHA

  公开号：EP0653492A2

  公开（公告）日：1995-05-17

  A process is provided for separating individual microorganisms either from a support such as soil particles to which they are adhered or from a flock in which the microorganisms are adhered together to form groups. The process involves enzymatically decomposing the natural polymeric adhesive which holds the microorganism to the support or to one another. After the individual microoganisms have been separated, they can be counted, or the proportion of one of the microorganisms within the microorganism population can be determined, or nucleic acids can be separated from the microorganisms. The enzyme may decompose polysaccharides, proteins or pectins and mixtures of enzymes may be used. Suitable enzymes for bringing about the separation of the individual organisms comprise cellulase, hemicellulase, glucuronidase, amylase, protease and pectinase.

  本发明提供了一种工艺，可将单个微生物从其粘附的土壤颗粒等支撑物中分离出来，或从微生物粘附在一起形成群体的菌群中分离出来。这个过程包括用酶分解将微生物粘附在支撑物上或相互粘附在一起的天然聚合物粘合剂。分离出单个微生物后，可对其进行计数，或确定微生物种群中某一微生物的比例，或从微生物中分离出核酸。酶可以分解多糖、蛋白质或果胶，也可以使用酶的混合物。适用于分离单个生物的酶包括纤维素酶、半纤维素酶、葡糖醛酸酶、淀粉酶、蛋白酶和果胶酶。