2-chloro-N⁶-cyclopentyladenine | 135394-21-7

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 咪唑并嘧啶类
• 英文名称: 2-chloro-N⁶-cyclopentyladenine
• 英文别名: 2-chloro-6-cyclopentylamino-9H-purine；2-chloro-N-cyclopentyl-7H-purin-6-amine
• CAS: 135394-21-7
• 化学式: C₁₀H₁₂ClN₅
• 分子量: 237.692
• InChiKey: FOJLQUHXPULJHJ-UHFFFAOYSA-N

物化性质

• 沸点：
  357.4±52.0 °C(Predicted)
• 密度：
  1.68±0.1 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  2.6
• 重原子数：
  16
• 可旋转键数：
  2
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.5
• 拓扑面积：
  66.5
• 氢给体数：
  2
• 氢受体数：
  4

反应信息

• 作为反应物：
  描述：

  2-chloro-N⁶-cyclopentyladenine 在 potassium carbonate 作用下， 以 N,N-二甲基甲酰胺 为溶剂， 反应 3.0h， 生成 6-(Cyclopentylamino)-2-[(3-hydroxypropyl)amino]-9-isopropylpurine
  参考文献：
  名称：

  Docking-Based Development of Purine-like Inhibitors of Cyclin-Dependent Kinase-2

  摘要：

  The cell division cycle is controlled by cyclin-dependent kinases (cdk), which consist of a catalytic subunit (cdk1-cdk8) and a regulatory subunit (cyclin A-H). Purine-like inhibitors of cyclin-dependent kinases have recently been found to be of potential use as anticancer drugs. Rigid and flexible docking techniques were used for analysis of binding mode and design of new inhibitors. X-ray structures of three (ATP, olomoucine, roscovitine) cdk2 complexes were available at the beginning of the study and were used to optimize the docking parameters. The new potential inhibitors were then docked into the cdk2 enzyme, and the enzyme/inhibitor interaction energies were calculated and tested against the assayed activities of cdk1 (37 compounds) and cdk2 (9 compounds). A significant rank correlation between the activity and the rigid docking interaction energy has been found. This implies that (i) the rigid docking can be used as a tool for qualitative prediction of activity and (ii) values obtained by the rigid docking technique into the cdk2 active site can also be used for the prediction of cdk1 activity. While the resulting geometries obtained by the rigid docking are in good agreement with the X-ray data, the flexible docking did not always produce the same inhibitor conformation as that found in the crystal.

  DOI：

  10.1021/jm990506w
• 作为产物：
  描述：

  2,6-二氯嘌呤 在 tris-(dibenzylideneacetone)dipalladium(0) 、 potassium tert-butylate 、 N,N-二异丙基乙胺 、 2-二环己基磷-2,4,6-三异丙基联苯 作用下， 以 四氢呋喃 、 异丙醇 、 叔丁醇 、 正丁醇 为溶剂， 反应 36.0h， 生成 2-chloro-N⁶-cyclopentyladenine
  参考文献：
  名称：

  二氨基嘌呤化学型的药物化学优化：寻找布氏锥虫抑制剂的先导物。

  摘要：

  非洲人类锥虫病 (HAT) 或昏睡病是由原生动物寄生虫布氏锥虫引起的，并通过受感染的采采蝇叮咬传播。如果不治疗，这种疾病被认为是致命的。为了鉴定针对布氏锥虫的新化学型，之前我们鉴定了 797 种有效的激酶靶向抑制剂，这些抑制剂分为 59 个簇和 53 种单一化合物，其选择性至少是 HepG2 细胞的 100 倍。从这组命中中，鉴定了一组二氨基嘌呤衍生的化合物。在此，我们报告了我们的药物化学研究，涉及探索围绕高通量筛选 (HTS) 命中之一N 2 -(噻吩-3-基)-的结构-活性和结构-性质关系N 6 -(2,2,2-三氟乙基)-9 H-嘌呤-2,6-二胺( 1，NEU-1106)。这项工作导致鉴定出一种有效的先导化合物（4aa，NEU-4854），其具有改善的体外吸收、分布、代谢和排泄 (ADME) 特性，并已进展为体外抗寄生虫活性的概念验证翻译到体内功效。

  DOI：

  10.1021/acs.jmedchem.0c01017

文献信息

• Purine Derivatives, Compositions Containing Them and Use Thereof
  申请人：CARREZ Chantal

  公开号：US20080119467A1

  公开（公告）日：2008-05-22

  Purine derivatives of formula (IA1) or (IB1), compositions containing them and use thereof as medicinal products, in particular in oncology, are described

  配方（IA1）或（IB1）的嘌呤衍生物，含有它们的组合物以及将其用作药物产品，特别是在肿瘤学中的用途被描述。
• Anticancer potentiating effect and downregulation of <scp>PD‐L1</scp> expression: Study on the 2‐[( <i>p</i> ‐fluorophenyl)amino]‐ <scp>6‐substituted‐9</scp> <i>H</i> ‐purine analogues as novel <scp>CHK1</scp>  inhibitors
  作者：Xuanzhen Chen、Meng Wang、Xiaowei Wang、Junyi Liu、Zhili Zhang、Chao Tian

  DOI：10.1111/cbdd.14156

  日期：2023.3

  In this study, a series of 2-[p-fluorophenyl]-6-substituted-9H-purine analogues were designed and synthesized as CHK1 inhibitors, among which compound b22 was the most potent. b22 exhibited nearly no antiproliferative activity toward HT29 cells and displayed a significant antitumor potentiating effect on HT29 cells when treated in combination with gemcitabine (Gem). A time-dependent assay found that

  本研究设计并合成了一系列2-[对氟苯基]-6-取代-9 H-嘌呤类似物作为CHK1抑制剂，其中化合物b22最为有效。b22对 HT29 细胞几乎没有抗增殖活性，当与吉西他滨 (Gem) 联合治疗时，b22 对 HT29 细胞显示出显着的抗肿瘤增强作用。时间依赖性测定发现，在添加b22之前用 Gem 处理 8 小时可达到最佳效果。此外，免疫荧光和 qPCR 结果表明，b22可以显着逆转 Gem 诱导的 PD-L1 上调，这表明b22具有双重作用在抗肿瘤增强和抗肿瘤免疫方面。
• N6,9-Disubstituted adenines: potent, selective antagonists at the A1 adenosine receptor
  作者：Robert D. Thompson、Sherrie Secunda、John W. Daly、Ray A. Olsson

  DOI：10.1021/jm00113a029

  日期：1991.9

  N6-Substituted 9-methyladenines are potent antagonists of the activation of A1 adenosine receptors. The present study assessed the effect of N6 and N-9 substituents on the binding of adenines to the A1 and A2 receptors, respectively, of rat brain cortex and striatum and also on the antagonism of the A2 receptor mediated stimulation of the adenylate cyclase of PC12 cells by N-ethyladenosine-5'-uronamide. The potency ranking of 9-substituted adenines varied directly with the hydrophobicity of the substituent: cyclopentyl > phenyl > tetrahydrofuryl > methyl > 2-hydroxyethyl. The 9-substituted adenines showed little selectivity for either receptor and the R enantiomer of N6-(1-phenyl-2-propyl)-9-methyladenine was only 4-fold more potent than the S enantiomer at the A1 receptor. An N6-cyclopentyl substituent increased potency at the A1 receptor and decreased potency at the A2 receptor, resulting in selectivity for the A1 receptor of up to 39-fold. The N6-cyclophenyl group completely overshadowed the effect of the hydrophobicity of the 9-substituent. A 2-chloro substituent did not alter the potency of an N6-substituted 9-methyladenine.